Regulation on new regulation in photoreceptor signal transduction

光感受器信号转导新规定

• 批准号: 08680688
• 负责人: TSUBOI Seiji
• 金额: $ 1.6万
• 依托单位: Okayama University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1996
• 资助国家: 日本
• 起止时间: 1996 至 1997
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08680688/
• 关键词: glutathione; S- (12 -dicarboxyethyl) glutathione; acetoaminophen; hepatotoxicity; g-glutamylcysteine synthetase; yeast; cysteine; cystathionine b-synthetase; 還元型グルタチオン; 酸化型グルタチオン; 活性酸素; 生体防御; 蛍光誘導体; HPLC; Pγ; Pγ Kinase; リン酸化; 視細胞; Ca結合蛋白

(1) Protective effect of S- (1,2-dicarboxyethyl) glutathione (DCE-GS) and its esters on acetoaminophen-induced hepatotoxicity in ratsThe pretreatment of DCE-GS triester prevented acetoaminophen-induced hepatotoxicity. It was found that the DCE-GS triester administration inhibited the GSH depletion caused by acetoaminophen, suggesting that the protective effect of DCE-GS triester on acetoaminophen-induced hepatotoxicity was due to the retention of hepatic GSH level.(2) Elevation effect of DCE-GS triester on glutathione levelThe effect of DCE-GS on GSH synthesis, especially g-glutamylcysteine synthetase, was studied in rat liver homogenate. In conclusion, DCE-GS promoted the hepatic GSH synthesis due to activation g-glutamylcysteine synthetase.(3) DCE-GS in YeastDCE-GS was found in Saccharomyces cerevisiae. The DCE-GS synthesizing enzyme was purified from yeast. The molecular mass of the enzyme was 49 kDa.(4) Contents of cysteine, glutathione and their related comounds as well as related enzyme activities in Saccharomyces serevisiae cultured under aerobic and anaerobic ocnditionsYeast grown aerobically increased activities of defensive enzymes against an oxygen stress like catalase and superoxide dismutase, and levels of the protective comounds cysteine and glutathione. The elevated synthesis of glutathione could be associated to the increased levels of cysteine which in its turn was found to be controlled by the oxygen dependent activation o cystathionie b-synthetase

(1)S-(1，2-二羧乙基)谷胱甘肽及其酯类对乙酰氨基酚所致大鼠肝毒性的保护作用结果表明，DCE-GS三酯可抑制乙酰氨基酚所致的GSH耗竭，提示DCE-GS三酯对乙酰氨基酚所致肝损伤的保护作用可能与其保留肝脏GSH水平有关。(2)DCE-GS三酯对大鼠肝匀浆GSH合成的影响。综上所述，DCE-GS通过激活g-谷氨酰半胱氨酸合成酶促进了肝脏GSH的合成。(3)在酿酒酵母中发现了YeastDCE-GS中的DCE-GS。从酵母中纯化了DCE-GS合成酶。(4)在好氧和厌氧条件下培养的Serevisiae Saccharmyces serevisiae的半胱氨酸、谷胱甘肽及其相关酶的含量和相关酶的活性。谷胱甘肽合成的增加可能与半胱氨酸水平的增加有关，而半胱氨酸水平的增加又被发现是由胱硫醚b-合成酶的氧依赖激活控制的。

项目成果

T.Sakaue et al.: "Protective Effect of S-(1,2-Dicarboxyethyl) glutathione,an Intrins Tripeptide in Liver,Heart and Lens,and Its Esters on APAP-." Biol.Pharm.Bull. 19(9). 1216-1219 (1996)

T.Sakaue 等人：“S-(1,2-二羧乙基) 谷胱甘肽（一种肝脏、心脏和晶状体中的内含三肽）及其酯对 APAP- 的保护作用。”

Takahiro Sakaue: "Protective effect of S- (1,2-dicarboxy-ethyl) glutathione, an intrinsic tripeptid in liver, heart and lens, and its esters on acetoaminophen-induced hepatotoxicity in rats" Bio.Pharm.Bull.19 (9). 1216-1219 (1996)

Takahiro Sakaue：“S-（1,2-二羧基乙基）谷胱甘肽（肝脏、心脏和晶状体中的一种内在三肽）及其酯对乙酰氨基酚诱导的大鼠肝毒性的保护作用”Bio.Pharm.Bull.19 (9

N.Nakagawa et al.: "S-(11,2-Dicarboxyethyl) glutathione,Intrinsic Tripeptide in Liver Heart,and Lens,elevates g-glutamylcysteine stnthetase Activity" Protein and Peptide Letters. 3 (6). 355-369 (1996)

N.Nakakawa 等人：“S-(11,2-二羧乙基) 谷胱甘肽、肝心和晶状体中的内在三肽，提高 g-谷氨酰半胱氨酸合成酶活性”蛋白质和肽快报。

Shuji Matsumoto: "Determination of Glutathione and Glutathione Disulfide in Biological Samples using Acrylonitrile as a Thiol-Bloking Reagent" Analytical Sciences. 12. 91-95 (1996)

Shuji Matsumoto：“使用丙烯腈作为硫醇阻断试剂测定生物样品中的谷胱甘肽和谷胱甘肽二硫化物”分析科学。

Naoko Nakagawa: "S- (1,2-Dicarboxyethyl) glutathione, intrinsic tripeptide in liver, heart and lens, elevates g-glutamylcysteine synthetase activity" Protein and Peptide Letters. 3 (6). 355-369 (1996)

Naoko Nakakawa：“S-（1,2-二羧乙基）谷胱甘肽是肝脏、心脏和晶状体中的内在三肽，可提高 g-谷氨酰半胱氨酸合成酶活性”《蛋白质与肽快报》。