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気孔開閉の分子機構をモデルとした受粉反応時の花粉吸水における膨圧調節機構の解明

以气孔开闭分子机制为模型阐明授粉反应过程中花粉吸水膨胀压调节机制

基本信息

批准号：
22KJ0158
负责人：
林真妃
金额：
$ 2.33万
依托单位：
Tohoku University
依托单位国家：
日本
项目类别：
Grant-in-Aid for JSPS Fellows
财政年份：
2023
资助国家：
日本
起止时间：
2023-03-08 至 2024-03-31
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22KJ0158/
关键词：
花粉吸水イオン輸送アブラナ科

项目摘要

受粉時、花粉は雌しべ柱頭に存在する乳頭細胞から水を受け取り吸水する。花粉吸水は花粉管発芽と伸長に必須であるため、植物が受精を達成し、次世代を残すために重要である。乳頭細胞上に付着した花粉は種間不和合性や種内不和合性により、受精可能な花粉が選択されたのち吸水させる。吸水可能な花粉を乳頭細胞が認識する自他認識機構から、その下流で花粉吸水を誘導する乳頭細胞内のシグナル伝達は理解されつつあるが、未だどのように乳頭細胞内の水が花粉へと移行するのか、吸水がされない花粉ではどのように水の移行が止まるのか、といったシグナル伝達末端の理解は進んでいない。申請者は、植物細胞の膨圧制御が花粉吸水に関与すると仮説を立て、膨圧制御に重要なイオン輸送体が花粉吸水時に機能しているかを調べている。前年度に乳頭細胞で高発現するイオン輸送体をシロイヌナズナの発現データベースより選抜し、今年度ではその遺伝子破壊株の表現型解析から、種子形成が野生株と比較して低下する株を見出した。また、イオン輸送体開口、閉鎖化合物ライブラリーを用いて、自家不和合性を示すアブラナ(Brassica rapa)の雌しべ内への花粉管侵入の様子が変化する化合物スクリーニングを行った。現在ライブラリー70種の化合物の中から自家不和合性に変化を与える化合物を数点選抜した。シロイヌナズナのイオン輸送体候補の変異株の種子形成の変化が乳頭細胞側の花粉吸水に対する影響かを調べると共に、アブラナの自家不和合性に影響を与える化合物の標的探索を行うことで、アブラナ科の花粉吸水に機能する乳頭細胞のイオン輸送体を同定する。

During pollination, there is a milk cell in the pollination column. The water is collected and absorbed. The elongation of pollen tube buds in the water-absorbing pollen tube must be completed, the fertilization of the plant must be completed, and the residual plants of the next generation must be important. Milk milk cells are covered with interspecific incompatibility and intra-species incompatibility, and fertilization may result in the selection of pollens and water absorbents. Water absorption may cause pollen and milk cells to learn from other institutions or institutions. The water absorption of pollen leads to the understanding of intracellular water absorption of milk cells. At the end of the day, we understand that we are in the process of making progress. The applicant and the plant cell expand to control the water absorption of pollen and to set up and control the important carrier of pollen. When the pollen absorbs water, the machine can change the temperature. In the previous year, there was a high level of breast cancer cells. In the previous year, there was a high level of breast cancer cells in the previous year. In the previous year, there was a high level of breast cancer cells in the previous year. In the previous year, the number of milk cells was higher than that in the previous year, and the number of wild plants was lower. The opening of the transporter, the chemical composition of the compound, and the incompatibility of the compound show that the pollen tube invades into the female and the pollen tube invades. The chemical compound is not compatible with each other. Now you can select the number of compounds that are not compatible with each other. This is the first step in the study of the development of the plant in which the phenotype is changed. The cells of the milk, the water absorption of the pollen, the water absorption of the pollen, the incompatibility of the plant, the exploration of the chemical compound, the water absorption of the pollen, the water absorption of the milk, the water absorption of the pollen, the water absorption of the milk, the water absorption of the pollen, the water absorption of the milk, the water absorption of the pollen, the water absorption of the milk, the water absorption of the pollen, the water absorption of the milk, the water absorption of the pollen, the water absorption of the milk, the water absorption of the pollen, the water absorption of the milk, the water absorption of the pollen, the water absorption of the milk, the water absorption of the pollen, the water absorption of the milk, the water absorption of the pollen, the water absorption of the milk, the water absorption of the pollen, the water absorption of the milk, the water absorption