Function of an intermembrane space chaperone complex in protein import into chloroplasts

膜间空间伴侣复合物在蛋白质输入叶绿体中的功能

• 批准号: 80770066
• 负责人: Dr. Joanna Tripp
• 金额: --
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2008
• 资助国家: 德国
• 起止时间: 2007-12-31 至 2013-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/80770066?language=en
• 关键词: Function; intermembrane; space; chaperone; complex

Protein import into chloroplasts occurs via a transport system hosted in the outer and inner envelope membrane. A translocation complex in the intermembrane space that consists of Toc64, a yet unidentified intermembrane space localized isoform of Hsp70 (imsHsp70), the DnaJ homolog Toc12 and Tic22 is thought to keep the incoming precursor in an unfolded state and to initiate the contact with the Tic translocon. I will attempt to identify imsHsp70 in the intermembrane space and use biochemical, cell biological and genetic methods to characterize the molecular function and the dynamic composition of the translocation complex in the intermembrane space.

蛋白质输入到叶绿体中是通过外被膜和内被膜中的运输系统进行的。膜间隙中的易位复合物由Toc64、尚未鉴定的膜间隙定位的Hsp70同种型（imsHsp70）、DnaJ同源物Toc12和Tic22组成，其被认为使进入的前体保持未折叠状态并启动与Tic易位子的接触。我将试图确定imsHsp70在膜间隙，并使用生物化学，细胞生物学和遗传学的方法来表征的分子功能和动态组成的易位复合物在膜间隙。

项目成果

Toc33 and Toc64-III cooperate in precursor protein import into the chloroplasts of Arabidopsis thaliana.

Toc33 和 Toc64-III 合作将前体蛋白导入拟南芥叶绿体

• DOI: 10.1111/pce.12030
• 发表时间: 2013
• 期刊: Plant, cell & environment
• 作者: Sommer M;Rudolf M;Tillmann B;Tripp J;Sommer M.S.;Schleiff E.
• 通讯作者: Schleiff E.