Mechanisms of anesthetics on in situ normotensive vs hypertensive rat mesenteric vascular smooth muscle transmembrane potentials

麻醉药对原位正常血压与高血压大鼠肠系膜血管平滑肌跨膜电位的影响机制

• 批准号: 09671549
• 负责人: YAMAZAKI Mitsuaki
• 金额: $ 1.92万
• 依托单位: Toyama Medical and Pharmaceutical University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 1998
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09671549/
• 关键词: vascular smooth muscle; transmembrane potential; isoflurane; sevoflurane; propofol; WKY rat; SHR rat; potassium channel; hypertension; normotension; vascular smooth muscle; anesthetics; membrane potential; peripheral vascular tone; splanchni

Since tight linear coupling exists between vascular smooth muscle (VSM) transmembrane potential (Em) and active VSM tone, such hyperpolarization reflects a vasorelaxation.In normotensive and hypertensive rats under basal anesthesia, we externalized a loop of jejunum containing mesenteric arteries and veins through a midline laparotomy and superfused them with physiological salt solution. Ems were recorded from in situ mesenteric resistance (arteries) and capacitance (veins) vessels with or without denervation (by 6- hydroxydopamine) using glass microelectrode. In normotensive rats, inhaled anesthetics (isoflurane, sevoflurane) and ketamine hyperpolarized in both mesenteric arteries and veins. Inhaled anesthetics attenuated both sympathetic neural and nonneural regulation of VSM transmembrane potentials ( and tone). Inhaled anesthetics hyperpolarized in hypertensive rats more negative than in normotensive rats.Selective inhibitors of each of four types of potassium channels known to exist in VSM were administered in superfusate of the vessel preparations (in normotensive rats) to assess their effects on isoflurane-mediated hyperpolarization. Isoflurane-mediated hyperpolarization of VSM can be attributed in part to an enhanced (or maintained) opening of calcium-activated and adenosine triphosphate sensitive potassium channels but not voltage-dependent or inward rectifier potassium channels.

由于血管平滑肌（VSM）跨膜电位（Em）和主动VSM张力之间存在紧密的线性耦合，这种超极化反映了血管舒张，在正常血压和高血压大鼠基础麻醉下，我们通过中线剖腹术将含有肠系膜动脉和静脉的空肠袢外化，并用生理盐水灌注。用玻璃微电极记录肠系膜血管的电阻（动脉）和电容（静脉），有或没有去神经（6-羟多巴胺）。在血压正常的大鼠中，吸入麻醉剂（异氟烷、七氟烷）和氯胺酮在肠系膜动脉和静脉中超极化。吸入麻醉剂减弱交感神经和非神经调节VSM跨膜电位（和音调）。吸入麻醉剂在高血压大鼠超极化更负比norm-pressure rates.Selective抑制剂的四种类型的钾通道已知存在于VSM中的每一个被管理在灌流液的血管制剂（在norm-pressure rates.to评估其对异氟烷介导的超极化。异氟烷介导的VSM超极化可部分归因于钙激活和三磷酸腺苷敏感性钾通道的增强（或维持）开放，但不是电压依赖性或内向整流钾通道。

项目成果

Yamazaki M,et al.: "Effects of volatile anesthetic agents on in situ vascular smooth muscle transmembrane potential in resistance- and capacitance-regulating blood vessels." Anesthesiology. 88・4. 1085-1095 (1998)

Yamazaki M 等人：“挥发性麻醉剂对电阻和电容调节血管中原位血管平滑肌跨膜电位的影响”88・4（1998）。

Yamazaki M,Stekiel TA,Boshjak ZJ,Kampine JP,Stekiel WJ.: "Effects of volatile anesthetic agents on in situ vascular smooth muscle transmenbrane potential in resistance-and capacitane-regulating blood vessels." Anesthesiology. Vol.88, No.4. 1085-1095 (1998

Yamazaki M，Stekiel TA，Boshjak ZJ，Kampine JP，Stekiel WJ.：“挥发性麻醉剂对阻力和容量调节血管中原位血管平滑肌跨膜电位的影响。”

Kokita N, et al.: "Potassium channel-mediated hyperpolarization of mesenteric vascular smooth muscle by isoflurane." Anesthesiology. 90・3 印刷中. (1999)

Kokita N 等人：“异氟烷介导的钾通道介导的肠系膜血管平滑肌超极化”，出版中的 90·3。

永川保 他: "ケタミンによるin situラット腸管膜動・静脈平滑筋の膜電位の変化." 北陸麻酔学雑誌. 32・1. 1-6 (1998)

Tamotsu Nagakawa 等人：“氯胺酮诱导的原位大鼠肠系膜和静脉平滑肌的膜电位变化。”Hokuriku Anesthesiology Journal 32, 1. 1-6 (1998)。

Nagakawa T,Yamazaki M,Hatakeyama N,Shibuya N,Ito Y.: "Effects of ketamine on in situ rat mesenteric vascular smooth muscle transmembrane potentials." Hokuriku J.Anesth.Vol.32, No.1. 1-6 (1998)

Nagakawa T，Yamazaki M，Hatakeyama N，Shibuya N，Ito Y.：“氯胺酮对原位大鼠肠系膜血管平滑肌跨膜电位的影响。”