Clinical roles of plasma phosphatidylcholine hydroperoxide-reducing activity in the development of pre-eclampsia

血浆磷脂酰胆碱过氧化氢还原活性在先兆子痫发生中的临床作用

• 批准号: 09671716
• 负责人: IWASAKI Katuhiko
• 金额: $ 1.79万
• 依托单位: Tokai University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 1999
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09671716/
• 关键词: Lipidperoxide; lipid peroxide reducing enzyme; apolipoprotein B100; Apolipoprotein A1; Pre-eclampsia; アポリポプロテインA1

Plasma lipid peroxide has been known as risk factor for atherogenesis, because lipid peroxide attacks endothelial cells. We found that blood plasma possess lipid peroxide reducing activity other than plasma glutathione peroxidase, a major lipid peroxide reducing enzyme. In this study, we initially purified this lipid peroxide reducing protein from human blood plasma and successfully isolated apolipoprotein A1 and B100 as phosphatidylcholine hydroperoxide reducing proteins. In order to clarify the pathophysiological roles of the activity, we have developed an assay method to measure plasma phosphatidylcholine hydroperoxide reducing activity using synthetic 1-pdtoyl-2-nnoleoyl-phosphatidylchonne hydroperoxide (PLPC-OOH) as the substrate. By using this method, PLPC-OOH reducing activity was determined in the plasma of 53 normal pregnancies, 11 patients of pre-eclampsia, 18 pre-stage and 11 high-risk groups for pre-eclampsia. PLPC-OOH reducing activity was greater in the high-risk group than normal pregnancy, while lipid peroxide level was greater in the pre-eclamptic patients. The present results suggested an important role(s) for PLPC-OOH reducing activity in elimination of the plasma lipid hydroperoxide, and further involvement in the development of pre-eclampsia.

血浆脂质过氧化被认为是动脉粥样硬化的危险因素，因为脂质过氧化会攻击内皮细胞。我们发现血浆具有除血浆谷胱甘肽过氧化物酶外的脂质过氧化还原活性，这是一种主要的脂质过氧化还原酶。在本研究中，我们首先从人血浆中纯化了这种脂质过氧化还原蛋白，并成功分离出作为磷脂酰胆碱过氧化氢还原蛋白的载脂蛋白A1和B100。为了阐明活性的病理生理作用，我们开发了一种测定血浆磷脂酰胆碱过氧化氢还原活性的方法，使用合成的1-pdtoyl-2-nnoleoyl-磷脂酰胆碱过氧化氢（PLPC-OOH）作为底物。采用该方法测定了53例正常妊娠、11例子痫前期、18例子痫前期和11例子痫前期高危人群血浆中PLPC-OOH的还原活性。高危组的PLPC-OOH降低活性高于正常妊娠，而子痫前期患者的过氧化脂质水平更高。目前的结果表明，PLPC-OOH降低活性在消除血浆脂质过氧化氢中起重要作用，并进一步参与先兆子痫的发展。

项目成果

加藤,俊一: "東海大学における臍帯血移植と臍帯血バンク" 今日の移植. 10・2. 411-414 (1997)

加藤俊一：“东海大学的脐带血移植和脐带血库”《今日移植》10・2（1997）。

岩崎克彦: "妊婦のかぜ症候群"周産期医学. 29(増刊号). 73-77 (1999)

Katsuhiko Iwasaki：“孕妇感冒综合症”29（特刊）73-77（1999）。

Murano T, Makino H, Yasui I, Arai T, Hirasawa T, Koido S, Iwasaki K, Makino T.: "Continuous anesthesia at the parturition of patient with Moya Moya disease using double catheter method."Kanagawa Dist. J. Jap. Obest. Gynecol. Soc.. 34(1). 61-64 (1997)

Murano T、Makino H、Yasui I、Arai T、Hirasawa T、Koido S、Iwasaki K、Makino T.：“使用双导管法对 Moya Moya 病患者分娩时进行持续麻醉。”神奈川县

Hayashida K, Iwasaki K, Makino T, Yamamoto Y.: "Plasma phspholipid hydroperoxide reducing activity and pre-eclampsia."The world of Obest. Gynelcol. 51(10). 939-943 (1999)

Hayashida K、Iwasaki K、Makino T、Yamamoto Y.：“血浆磷脂氢过氧化物降低活性和先兆子痫。”Obest 的世界。

Ryuichi Mashima: "Reduction of phosphatidylcholine hydroperoxide by apolipoprotein A1:purification of the hydroperoxide-reducing protein from human blood plasma" J.Lipid Res.39. 1133-1140 (1998)

Ryuichi Mashima：“载脂蛋白 A1 还原氢过氧化磷脂酰胆碱：从人血浆中纯化氢过氧化物还原蛋白”J.Lipid Res.39。