Effect of prenatal ethano exposure on myelin proteins and discrimination learning in rat offspring

产前乙醇暴露对大鼠后代髓磷脂蛋白和辨别学习的影响

• 批准号: 09671710
• 负责人: KOJIMA Hisako
• 金额: $ 1.79万
• 依托单位: Kitasato University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 1999
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09671710/
• 关键词: Fetal Alcohol Syndrome; Clinical Animal Model; Northern Blot; Myelin Preteins; Discrimination Learning; ノーザンブロット; モデル動物; 伝達物質合成酵素; 胎仔性アルコール症候群; 神経伝達物質

We previously found that developmental expression of mRNAs coding for myelin basic protein (MBP) and 2', 3', cyclic nucleotide 3'-phosphodiesterase (CNP) of rat offspring exposed to ethanol in uterus was affected. In this study, we examined the effects of prenatal ethanol exposure on myelin protein, proteolipid protein (PLP) and myelin associated glycoprotein (MAG), and discrimination learning ability in the rat offsprings. Subclinical dose of ethanol was administered intraperitoneally to pregnant rats. The levels of mRNA coding for PLP and MAG at various developing periods were examined by Northern blot analysis of total cellular RNAs. The expressions of PLP and MAG mRNA in rats prenatally exposed to ethanol were delayed as to the developmental pattern compared with those of control rats. It is suggested that the expression patterns of MBP and CNP were different with those of PLP and MAG. Taking together the present and previous findings, the prenatal ethanol exposure to rats affects all myelin proteins at their mRNA level, although the effects were different in different protein species. Rats were trained to discriminate between lamp-on and lamp-off states under an operant-type learning procedure. The discrimination ratio between rats prenatally exposed to ethanol and the control were not found, but the number of overall responses in rats exposed prenatally ethanol were higher than control rats. The differences between two groups were statistically significant (P<0.05).

我们之前发现，子宫内暴露于乙醇的大鼠后代的髓鞘碱性蛋白（MBP）和2',3‘，环核苷酸3’-磷酸二酯酶（CNP）编码mrna的发育表达受到影响。在本研究中，我们检测了产前乙醇暴露对大鼠后代髓磷脂蛋白、蛋白脂蛋白（PLP）和髓磷脂相关糖蛋白（MAG）以及辨别学习能力的影响。用亚临床剂量的乙醇腹腔注射妊娠大鼠。通过细胞总rna的Northern blot分析，检测了不同发育时期PLP和MAG的mRNA编码水平。与对照组相比，乙醇暴露大鼠PLP和MAG mRNA的表达在发育模式上有所延迟。提示MBP和CNP的表达模式与PLP和MAG的表达模式不同。综合目前和以往的研究结果，大鼠产前乙醇暴露在mRNA水平上影响了所有髓鞘蛋白，尽管不同蛋白种类的影响有所不同。在操作型学习过程中，训练大鼠区分开灯和关灯状态。未发现乙醇暴露大鼠与对照大鼠的鉴别率，但乙醇暴露大鼠的总体应答数高于对照大鼠。两组间差异有统计学意义（P<0.05）。