The effect of NO in the sesretion from nasal epithelial goblet cells.

NO（一氧化氮）是一种重要的炎症介质，影响许多关键的细胞功能。最近，高水平的一氧化氮生产已报道在鼻子或副鼻窦。然而一氧化氮在鼻气道中的作用尚不清楚。本实验研究了一氧化氮产生物（no7、no5、no12）对大鼠鼻上皮杯状细胞粘液分泌的影响。通过视频增强显微镜估计粘液分泌物的数量，这项技术使我们能够看到每个细胞的粘液颗粒的每次胞吐反应，并计算反应的数量。no5、no7和NOC 12的半衰期分别为25 min、7 min和100 min（在37 C， pH7.4条件下），均增加了杯状细胞的粘液分泌。然而，与基线分泌相比，6或600 μ m的NOCS并没有增加胞外反应。8 -溴- cgmp （3mm）也增加粘液释放。这些结果提示NO对鼻杯状细胞分泌的影响有限，且可能激活鸟苷酸环化酶。SP （substance P）是感觉C纤维的神经递质之一，被认为是杯状细胞分泌的强效激动剂。在这项研究中。SP的作用不受L-NAME和L-NNA的抑制，提示SP和NO可能通过不同途径诱导粘膜分泌。

NO (nitric oxide) is an important inflammatory mediators that affects many critical cell functions. Recently, high levels of NO production have been reported in the nose or paranasal sinuses. However the role of NO in the nasal airway remains to be clarified. In this study we investigated the effect NO generator (NOC7, NOC5, NOC12) in the mucous secretion from the rat nasal epithelial goblet cells. The amount of mucous secretions were estimated by video enhanced microscopy, this technique enables us to visualize each exocytotic response of the mucous granule from each cell and to count the number of the response. NOC5, NOC7 and NOC 12, the half life is 25 min, 7 min. 100 min respectively (under the condition of 37ﾟC, pH7.4), all increased mucous secretion from the goblet cells. However 6, or 600 muM of NOCS did not increase the exocytotic responses compared with baseline secretion. Eight-bromo-cGMP (3 mM) also increased mucus release. These results suggested that the effect of NO in the secretion from nasal goblet cells limited a narrow dosage, and might activate the guanylate cyclase. SP (substance P) is one of neurotransmitters of sensory C fibers, and known as a potent agonist to the goblet cell secretions. In this study. the effect of SP did not inhibited by L-NAME and L-NNA, indicate that SP and NO might induce the mucous secretion in the different pathway.