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The effect of NO in the sesretion from nasal epithelial goblet cells.

NO 在鼻上皮杯状细胞分泌中的作用。

基本信息

批准号：
09671739
负责人：
KAMIJO Atsushi
金额：
$ 1.92万
依托单位：
Yamanashi Medical University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1997
资助国家：
日本
起止时间：
1997 至 1998
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09671739/
关键词：
nitric oxide airway secretion mucus goblet cell 鼻粘膜上皮杯細胞

项目摘要

NO (nitric oxide) is an important inflammatory mediators that affects many critical cell functions. Recently, high levels of NO production have been reported in the nose or paranasal sinuses. However the role of NO in the nasal airway remains to be clarified. In this study we investigated the effect NO generator (NOC7, NOC5, NOC12) in the mucous secretion from the rat nasal epithelial goblet cells. The amount of mucous secretions were estimated by video enhanced microscopy, this technique enables us to visualize each exocytotic response of the mucous granule from each cell and to count the number of the response. NOC5, NOC7 and NOC 12, the half life is 25 min, 7 min. 100 min respectively (under the condition of 37ﾟC, pH7.4), all increased mucous secretion from the goblet cells. However 6, or 600 muM of NOCS did not increase the exocytotic responses compared with baseline secretion. Eight-bromo-cGMP (3 mM) also increased mucus release. These results suggested that the effect of NO in the secretion from nasal goblet cells limited a narrow dosage, and might activate the guanylate cyclase. SP (substance P) is one of neurotransmitters of sensory C fibers, and known as a potent agonist to the goblet cell secretions. In this study. the effect of SP did not inhibited by L-NAME and L-NNA, indicate that SP and NO might induce the mucous secretion in the different pathway.

一氧化氮（nitric oxide，NO）是一种重要的炎症介质，影响细胞的许多重要功能.最近，高水平的NO生产已被报道在鼻子或鼻旁窦。然而，NO在鼻气道中的作用仍有待澄清。本实验研究了NO发生器（NOC 7、NOC 5、NOC 12）对大鼠鼻上皮杯状细胞分泌粘液的影响。粘液分泌物的量通过视频增强显微镜估计，该技术使我们能够可视化每个细胞粘液颗粒的每次胞吐反应并计数反应的数量。NOC 5、NOC 7和NOC 12在37 ℃、pH7.4条件下的半衰期分别为25 min、7 min、100 min，均能促进杯状细胞分泌粘液。然而，与基线分泌相比，6或600 μ M的NOCS没有增加胞吐反应。8-溴-cGMP（3 mM）也增加粘液释放。提示NO对鼻杯状细胞分泌物的作用仅限于较窄的剂量范围，并可能激活鸟苷酸环化酶。P物质（substance P）是感觉C纤维的神经递质之一，是杯状细胞分泌物的强激动剂。本研究L-NAME和L-NNA不抑制SP的作用，提示SP和NO可能通过不同途径诱导粘液分泌。