The effect of NO in the sesretion from nasal epithelial goblet cells.

一氧化氮(NO)是一种重要的炎症介质，影响细胞的许多重要功能。最近，有报道称，鼻腔或副鼻窦中产生了大量的NO。然而，NO在鼻腔呼吸道中的作用仍有待阐明。本研究观察了NO生成物NOC7、NOC5、NOC12对大鼠鼻上皮杯状细胞黏液分泌的影响。粘液分泌量是通过视频增强显微镜估计的，这项技术使我们能够直观地看到每个细胞的粘液颗粒的每次吐出反应并计算反应的数量。NOC5、NOC7和NOC12，半衰期分别为25分钟、7分钟。分别在37ﾟC、pH 7.4条件下作用100min，均能促进杯状细胞的粘液分泌。然而，与基线分泌相比，6或600微米的NOCS并没有增加胞吐反应。8-溴-cGMP(3 MM)也能促进粘液释放。这些结果提示，NO对鼻杯状细胞分泌的影响是有限的，可能激活鸟苷环化酶。P物质(SP)是感觉C纤维的神经递质之一，是杯状细胞分泌物的有力激动剂。在这项研究中。L-NAME和L-NNA对SP的作用不受抑制，提示SP和NO可能通过不同途径诱导粘液分泌。

NO (nitric oxide) is an important inflammatory mediators that affects many critical cell functions. Recently, high levels of NO production have been reported in the nose or paranasal sinuses. However the role of NO in the nasal airway remains to be clarified. In this study we investigated the effect NO generator (NOC7, NOC5, NOC12) in the mucous secretion from the rat nasal epithelial goblet cells. The amount of mucous secretions were estimated by video enhanced microscopy, this technique enables us to visualize each exocytotic response of the mucous granule from each cell and to count the number of the response. NOC5, NOC7 and NOC 12, the half life is 25 min, 7 min. 100 min respectively (under the condition of 37ﾟC, pH7.4), all increased mucous secretion from the goblet cells. However 6, or 600 muM of NOCS did not increase the exocytotic responses compared with baseline secretion. Eight-bromo-cGMP (3 mM) also increased mucus release. These results suggested that the effect of NO in the secretion from nasal goblet cells limited a narrow dosage, and might activate the guanylate cyclase. SP (substance P) is one of neurotransmitters of sensory C fibers, and known as a potent agonist to the goblet cell secretions. In this study. the effect of SP did not inhibited by L-NAME and L-NNA, indicate that SP and NO might induce the mucous secretion in the different pathway.