THE ANALYSIS OF THE VKH DISEASE SPECIFIC ANTIGEN - SDS INSOLUBLE PROTEIN

VKH疾病特异性抗原-SDS不溶性蛋白的分析

• 批准号: 09671784
• 负责人: YAMAKI Kunihiko
• 金额: $ 1.92万
• 依托单位: AKITA UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 1998
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09671784/
• 关键词: Experimental VKH disease; Tyrosinase family protein; Reactivity of the T cells; T cell clone; Tyrosinse family Protein; 実験的VKHdisease; Tyrosinase family protein

We have been analysing the unsoluble fraction of melanocytes. We reported that tyrosinase family protein that is expressed specifically in the melanocytes can induce the experimental Vogt Koyanagi Harada disease(EVKH) in Lewis rat. In this study, we induced EVKH in pigmented rats. Clinicaly, some of the fundus shows so called 'sunset glow fundus' like appearance. histologically, markedly thickning of the choroid, Dalen-Fucks nodule and granulomataous inflammatory changes were found in the eye of the pigmented rats.Extra ocular sign of the inflammatory lesion of the skin and meningitis were also found in the pimented rats.The lymphocyte proliferation assay of the VKH disease patients was done. the lymphocytes of the VKH disease patients proliferated against the peptides derived from tyrosinase family proteins. Taken together, it is suggested that VKH disease is an autoimmune disease induced against the tyrosinase family proteins.

我们一直在分析黑素细胞的不溶部分。我们报道了在黑素细胞中特异表达的酪氨酸酶家族蛋白可以诱导Lewis大鼠实验性Vogt Koyanagi Harada病(EVKH)。在本研究中，我们在色素沉着的大鼠中诱导了EVKH。临床上，一些眼底表现为所谓的“夕阳辉光眼底”样外观。组织学上，色素组大鼠眼部脉络膜明显增厚，大理石结节明显增厚，肉芽肿性炎性改变，皮肤炎性损害和脑膜炎也有眼外征象。VKH患者的淋巴细胞在酪氨酸酶家族蛋白来源的多肽的作用下增殖。综上所述，VKH病是一种针对酪氨酸酶家族蛋白诱导的自身免疫性疾病。