The elucidation for the mechanism of induction on intractable uveoretinitis with heat-shock protein

热休克蛋白60来源于小肠结肠炎耶尔森菌（Yersinia HSP60）和牛视网膜热休克蛋白（Retina HSP60），此前通过免疫交叉反应和高度的共同抗原性鉴定，并在顽固性葡萄膜炎患者血清中检测到针对两者的特异性抗体。我们分离纯化了视网膜HSP60和耶尔森氏HSP60。在接种这些蛋白后，观察到大鼠的葡萄膜炎。提示视网膜和耶尔森菌HSP60有交叉反应。也有研究认为，口腔内复发性溃疡中链球菌HSP的65kDa可能通过免疫系统致敏参与白塞氏病的葡萄膜视网膜炎。我们检测了60 kDa热休克蛋白家族不同成员的血清抗体滴度，以及它们与热休克蛋白60在白塞氏病中的作用相关的意义。我们从患者口腔溃疡边缘分离出链球菌的HSP60和耶尔森氏菌、视网膜母细胞瘤和牛视网膜的HSP60，用ELISA法检测了这些HSP60与20例白塞氏病患者100倍稀释血清的反应。100倍稀释血清的抗HSP60抗体和抗视网膜HSP60抗体滴度均显著高于同样稀释的健康供者血清样本。ELISA抗体滴度测定结果显示，虽然各种hsp60具有共同的基本抗原性，但它们对白塞病患者血清中抗hsp60抗体的反应性不同。结果表明，不同hsp60的抗原性存在细微但显著的差异，它们具有共同的基本抗原性，并且分子量大致相同，提示视网膜和链球菌hsp60之间的免疫交叉反应以及相关的自身免疫反应可能参与了不可变性葡萄膜视网膜炎，特别是Bechet病的发展。少

Heat-shock protein 60 derived from Yersinia enterocolitica (Yersinia HSP60) and bovine retinal HSP (Retina HSP60) were previously identified by immunological cross reation and a high degree of common antigenicity, and a specific antibody against both was detected in the sera of intractable uveitis patients. We isolated and purified Retina HSP60 and Yersinia HSP60. Uveitis in rats following inoculation with these proteins was observed. Cross-reaction for both Retina and Yersinia HSP60 is suggested. It has been also suggested that the 65kDa of HSP of Streptococcus in recurrent aphthae within the oral cavity may be involved in the uveoretinitis of Behcet's disease, possibly through sensitization of the immune system. We examined serum antibody titers for various members of the 60 kDa family of HSPs and their implications relating to a role for HSP60s especialy focused in the Behcet's disease. We isolated the HSP60 of Streptococcus from the margin of oral aphthae in patientand the HSP60s o … More f Yersinia, retinoblastoma, and bovine retinat, and investigated the reaction of each of these HSP60s with 100-fold diluted serum samples from 20 Behcet's disease patients, with by ELISA. The anti-Streptococcus HSP60 antibody and anti-retinal HSP60 antibody titers of the 100-fold diluted serum were both significantly higher than those of similarly diluted serum samples from healthy donors. The results of ELISA antibody titer assay showed that, although the various HSP60s share a common basic antigenicity, they differed in reactivity to the anti-HSP60 antibodies in the sera of the Behcet's disease patients. The results indicate that subtle but significant differences exist in the antigenicity of the various HSP60s tested, all of which share a common basic antigenicity and are of approximately the same molecular weight, and suggest that an immuno cross-reaction between retinal and Streptococcal HSPs and a related autoimmune response may be involved in the development of intarctable uveoretinitis, especialy, Bechet's disease. Less