The elucidation for the mechanism of induction on intractable uveoretinitis with heat-shock protein

来自小肠结肠炎耶尔森氏菌的热休克蛋白60（Yersinia HSP 60）和牛视网膜HSP（Retina HSP 60）先前通过免疫交叉反应和高度的共同抗原性被鉴定，并且在难治性葡萄膜炎患者的血清中检测到针对两者的特异性抗体。我们分离并纯化了视网膜HSP 60和耶尔森氏菌HSP 60。在用这些蛋白质接种后的大鼠中观察到葡萄膜炎。建议视网膜和耶尔森氏菌HSP 60的交叉反应。也有人提出，口腔内复发性口疮中链球菌的65kDa HSP可能参与白塞氏病的葡萄膜视网膜炎，可能通过免疫系统的致敏。我们检测了60 kDa热休克蛋白家族的不同成员的血清抗体滴度，以及它们与热休克蛋白60的作用有关的意义，特别是在白塞氏病中。我们从口腔溃疡患者的龈缘分离出链球菌的HSP60， ...更多信息 fYersinia、视网膜母细胞瘤和牛视网膜，并通过ELISA研究这些HSP 60中的每一种与来自20名白塞氏病患者的100倍稀释的血清样品的反应。100倍稀释血清的抗链球菌HSP60抗体和抗视网膜HSP60抗体滴度均显著高于来自健康供体的类似稀释血清样品。ELISA抗体滴度测定结果表明，虽然各种HSP 60具有共同的基本抗原性，但它们对白塞病患者血清中抗HSP 60抗体的反应性不同。结果表明，各种HSP60的抗原性存在细微但显著的差异，所有这些HSP60具有共同的基本抗原性，并且具有大致相同的分子量，并且表明视网膜和链球菌HSPs之间的免疫交叉反应以及相关的自身免疫反应可能参与了不可治愈的葡萄膜视网膜炎，特别是Bechet病的发展。少

Heat-shock protein 60 derived from Yersinia enterocolitica (Yersinia HSP60) and bovine retinal HSP (Retina HSP60) were previously identified by immunological cross reation and a high degree of common antigenicity, and a specific antibody against both was detected in the sera of intractable uveitis patients. We isolated and purified Retina HSP60 and Yersinia HSP60. Uveitis in rats following inoculation with these proteins was observed. Cross-reaction for both Retina and Yersinia HSP60 is suggested. It has been also suggested that the 65kDa of HSP of Streptococcus in recurrent aphthae within the oral cavity may be involved in the uveoretinitis of Behcet's disease, possibly through sensitization of the immune system. We examined serum antibody titers for various members of the 60 kDa family of HSPs and their implications relating to a role for HSP60s especialy focused in the Behcet's disease. We isolated the HSP60 of Streptococcus from the margin of oral aphthae in patientand the HSP60s o … More f Yersinia, retinoblastoma, and bovine retinat, and investigated the reaction of each of these HSP60s with 100-fold diluted serum samples from 20 Behcet's disease patients, with by ELISA. The anti-Streptococcus HSP60 antibody and anti-retinal HSP60 antibody titers of the 100-fold diluted serum were both significantly higher than those of similarly diluted serum samples from healthy donors. The results of ELISA antibody titer assay showed that, although the various HSP60s share a common basic antigenicity, they differed in reactivity to the anti-HSP60 antibodies in the sera of the Behcet's disease patients. The results indicate that subtle but significant differences exist in the antigenicity of the various HSP60s tested, all of which share a common basic antigenicity and are of approximately the same molecular weight, and suggest that an immuno cross-reaction between retinal and Streptococcal HSPs and a related autoimmune response may be involved in the development of intarctable uveoretinitis, especialy, Bechet's disease. Less