The elucidation for the mechanism of induction on intractable uveoretinitis with heat-shock protein

小肠结肠炎耶尔森菌热休克蛋白60(Yersinia HSP60)和牛视网膜热休克蛋白60(Retina HSP60)是通过免疫交叉反应和高度的共同抗原性鉴定出来的，在难治性葡萄膜炎患者的血清中也检测到了针对这两种蛋白的特异性抗体。我们分离纯化了视网膜HSP60和耶尔森氏菌HSP60。对接种这些蛋白的大鼠葡萄膜炎进行了观察。视网膜和耶尔森氏菌HSP60都有交叉反应。也有人认为，口腔内复发性口疮中链球菌热休克蛋白65 kDa可能通过免疫系统的致敏作用参与了白塞病葡萄膜视网膜炎的发病。我们检测了60 kDa热休克蛋白家族不同成员的血清抗体效价，以及它们对热休克蛋白60的作用的影响，特别是在白塞病中的作用。我们从口腔口疮患者边缘分离到链球菌热休克蛋白60，从…中分离到热休克蛋白60。更多的耶尔森氏菌、视网膜母细胞瘤和牛视网膜，并用来自20名白塞病患者的100倍稀释的血清样本，用酶联免疫吸附试验(ELISA)研究每种HSP60的反应。100倍稀释血清的抗链球菌HSP60抗体和抗视网膜HSP60抗体效价均显著高于健康献血员同等稀释的血清样品。EL ISA抗体滴度测定结果表明，虽然不同的HSP60具有共同的基本抗原性，但它们对白塞病患者血清中的抗HSP60抗体的反应性不同。结果表明，不同HSP60的抗原性存在细微但显著的差异，它们都具有共同的基本抗原性，并且具有大致相同的分子量，提示视网膜和链球菌HSPs之间的免疫交叉反应和相关的自身免疫反应可能参与了难治性葡萄膜视网膜炎的发生，特别是Bechet病。较少

Heat-shock protein 60 derived from Yersinia enterocolitica (Yersinia HSP60) and bovine retinal HSP (Retina HSP60) were previously identified by immunological cross reation and a high degree of common antigenicity, and a specific antibody against both was detected in the sera of intractable uveitis patients. We isolated and purified Retina HSP60 and Yersinia HSP60. Uveitis in rats following inoculation with these proteins was observed. Cross-reaction for both Retina and Yersinia HSP60 is suggested. It has been also suggested that the 65kDa of HSP of Streptococcus in recurrent aphthae within the oral cavity may be involved in the uveoretinitis of Behcet's disease, possibly through sensitization of the immune system. We examined serum antibody titers for various members of the 60 kDa family of HSPs and their implications relating to a role for HSP60s especialy focused in the Behcet's disease. We isolated the HSP60 of Streptococcus from the margin of oral aphthae in patientand the HSP60s o … More f Yersinia, retinoblastoma, and bovine retinat, and investigated the reaction of each of these HSP60s with 100-fold diluted serum samples from 20 Behcet's disease patients, with by ELISA. The anti-Streptococcus HSP60 antibody and anti-retinal HSP60 antibody titers of the 100-fold diluted serum were both significantly higher than those of similarly diluted serum samples from healthy donors. The results of ELISA antibody titer assay showed that, although the various HSP60s share a common basic antigenicity, they differed in reactivity to the anti-HSP60 antibodies in the sera of the Behcet's disease patients. The results indicate that subtle but significant differences exist in the antigenicity of the various HSP60s tested, all of which share a common basic antigenicity and are of approximately the same molecular weight, and suggest that an immuno cross-reaction between retinal and Streptococcal HSPs and a related autoimmune response may be involved in the development of intarctable uveoretinitis, especialy, Bechet's disease. Less