Analysis of dendritic cells from patients with hematologic neoplasms

血液肿瘤患者树突状细胞的分析

• 批准号: 09671138
• 负责人: OGATA Kiyoyuki
• 金额: $ 1.73万
• 依托单位: Nippon Medical School
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 1999
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09671138/
• 关键词: Dendritic cells; Hematologic neoplasms; Immunotherapy; 樹上細胞

Our subjects were patients who had been diagnosed with acute mayeloid leukemia (AML), non-Hodgkin's lymphoma (NHL), Hodgkin's disease (HD) and myelodysplastic syndromes (MDS). Peripheral blood (PB) was obtained from the patients at diagnosis, except for the AML patients, who were in continued complete remission. Dendritic cells (DC) were purified from PB by Ficoll density centrifugation, T rosetting, dish adherence and metrizamide density centrifugation. DC function was assessed by their capacity to stimulate allogeneic T cells. Expression of functionally important molecules on CD83-positive DC, I.e., adhesion molecules (CD11a, CD54, CD58), co-stimulatory molecules (CD80, CD86, CD40) and HLA (DR, DP, DQ), was analyzed by two-color flow cytometry. The function of DC from NHL and HD patients varied among patients, but was reduced in most patients when compared with the DC function of normal volunteers (NV). Expression of functionally important molecules on DC did not differ significantly between NV and patients with NHL or HD. DC function of AML patients was at least equal to that of NV. The DC function of most MDS patients was similar to that of NV. Some DC were found to be derived from an abnormal clone in MDS patients when analyzed by fluorescence in situ hybridization.

我们的研究对象是被诊断为急性髓系白血病（AML）、非霍奇金淋巴瘤（NHL）、霍奇金病（HD）和骨髓增生异常综合征（MDS）的患者。外周血（PB）是从诊断时的患者，除了AML患者，谁是在持续完全缓解。采用Ficoll密度离心法、T花环法、贴壁法和甲泛葡胺密度离心法从外周血中分离纯化树突状细胞（DC）。DC功能通过其刺激同种异体T细胞的能力来评估。CD83阳性DC上功能重要分子的表达，即，采用双色流式细胞术检测细胞表面粘附分子（CD11a、CD54、CD58）、共刺激分子（CD80、CD86、CD40）和HLA（DR、DP、DQ）的表达。NHL和HD患者的DC功能在患者之间存在差异，但与正常志愿者（NV）的DC功能相比，大多数患者的DC功能降低。DC上功能重要分子的表达在NV和NHL或HD患者之间没有显著差异。AML患者的DC功能至少与NV相当。大多数MDS患者的DC功能与NV相似。荧光原位杂交分析发现，MDS患者中的一些DC来源于异常克隆。