Study for improvement of small bowel transplantation by donor bone marrow transplantation or total body irradiation

在器官移植中，移植物的修饰据说可以减少排斥反应。因此。我们阐明了移植物改良，如供体全身照射(TBI)或受者骨髓移植(BM)是否提高了小肠移植的移植物存活率。近交系大鼠：BN和LEW分别用于供体或受体。原位全小肠移植前，供者TB！或进行骨髓移植。比较移植物存活和移植物抗宿主病(GVHD)的发病情况。(1)BN*LEW：对照组中位存活天数(MSD)为20天，7只大鼠中有2只出现暂时性移植物抗宿主病(GVHD)，存活时间长。对照组大鼠全部死于排斥反应。TBI组和BM组移植物存活率分别降至12d和15.5d(MSD)。(2)Lew-BN：对照组大鼠的中位存活天数(MSD)为29天，对照组大鼠全部死于GVHD。一组人死于排斥反应。BM组移植肾存活时间为12天(MSD)。BM组大鼠全部死于严重OVID，未发生严重GVHD的大鼠存活率下降，原因是供者TB！只减少OVH反应，不减少排斥反应。受者骨髓移植增加了移植物的抗原性，移植物的存活率恶化，增加了GVHD的风险。综上所述，移植物的改良如供体的TBI或BM并不能减少排斥反应。

In organ transplantation, graft modification said to be reduce the rejection. Therefore. we clarify whether graft modification such as donor total body irradiation (TBI) or recipient bone marrow transplantation (to the donor) (BM) improve the graft survival in small bowel transplantation.Inbred rats : BN and LEW were used for a donor or a recipient. before orthotopic total small bowel transplantation, donor TB! or BM was performed. We compared the graft survival and onset of graft-versus-host disease (GVHD).(1) BN*LEW : In control groups, median survival day (MSD) was 20 days, and 2 of 7 rats showed temporally GVHD, which had long term survival. All rats in control group died of rejection. The graft survival in TBI group and BM group decreased to 12 days, 15.5 days (MSD), respectively. None of rats iii both groups showed GVHD.Histologically the findings of the rejection were more slight iii non-GVHD rats than GVHD rats.(2)LEW-BN : In control groups, median survival day (MSD) was 29 days, and all rats in control group died of GVHD.The graft survival in TBIgroup improved to 69 days (MSD), and all rats in TB! group died of rejection. The graft survival in BM group was 12 days(MSD). All of rats in BM group showed and died of severe OVID.Survival of the rats without severe GVHD decreased, because that donor TB! only reduce the OVH - reaction, but not reduce the rejection. Recipients bone marrow transplantation increase the antigenicity of the graft, therefore graft survival was worsened and increased the risk of GVHD.In summary, graft modification such as donor TBI or BM can not reduce the rejection.