Study for improvement of small bowel transplantation by donor bone marrow transplantation or total body irradiation

通过供体骨髓移植或全身照射改善小肠移植的研究

• 批准号: 09671205
• 负责人: FURUYA Tomoki
• 金额: $ 1.86万
• 依托单位: Akita University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 1998
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09671205/
• 关键词: Small bowel transplantation; Bone marrow transplantation; Total body irradiation

In organ transplantation, graft modification said to be reduce the rejection. Therefore. we clarify whether graft modification such as donor total body irradiation (TBI) or recipient bone marrow transplantation (to the donor) (BM) improve the graft survival in small bowel transplantation.Inbred rats : BN and LEW were used for a donor or a recipient. before orthotopic total small bowel transplantation, donor TB! or BM was performed. We compared the graft survival and onset of graft-versus-host disease (GVHD).(1) BN*LEW : In control groups, median survival day (MSD) was 20 days, and 2 of 7 rats showed temporally GVHD, which had long term survival. All rats in control group died of rejection. The graft survival in TBI group and BM group decreased to 12 days, 15.5 days (MSD), respectively. None of rats iii both groups showed GVHD.Histologically the findings of the rejection were more slight iii non-GVHD rats than GVHD rats.(2)LEW-BN : In control groups, median survival day (MSD) was 29 days, and all rats in control group died of GVHD.The graft survival in TBIgroup improved to 69 days (MSD), and all rats in TB! group died of rejection. The graft survival in BM group was 12 days(MSD). All of rats in BM group showed and died of severe OVID.Survival of the rats without severe GVHD decreased, because that donor TB! only reduce the OVH - reaction, but not reduce the rejection. Recipients bone marrow transplantation increase the antigenicity of the graft, therefore graft survival was worsened and increased the risk of GVHD.In summary, graft modification such as donor TBI or BM can not reduce the rejection.

在器官移植中，移植物修饰可以减少排斥反应。因此。我们阐明了在小肠移植中，移植物修饰如供体全身照射（TBI）或受体骨髓移植（对供体）（BM）是否能提高移植物存活率。在原位全小肠移植之前，供体肺结核！或进行BM。我们比较了移植物存活率和移植物抗宿主病（GVHD）的发生。(1)BN*LEW：对照组中位生存日（median survival day，MSD）为20 d，7只大鼠中有2只出现暂时性GVHD，长期存活。对照组大鼠全部死于排斥反应。TBI组和BM组移植物存活时间分别为12天和15.5天（MSD）。两组大鼠均未发生GVHD，非GVHD大鼠的排斥反应较GVHD大鼠轻。（2）LEW-BN：对照组中位生存日（MSD）为29天，对照组全部死于GVHD，TBI组移植物存活时间为69天，TB组全部死于GVHD。一群人死于排斥。BM组移植物存活12天（MSD）。BM组大鼠均出现严重奥维德感染并死亡，无严重GVHD的大鼠存活率下降，原因是供体肺结核！仅减少OVH反应，但不减少排斥反应。受者骨髓移植增加了移植物的抗原性，使移植物存活率下降，增加了GVHD的风险。