Immunophotodetection of Human Colon Cancer by ICCD Camera

ICCD 相机对人类结肠癌的免疫光电检测

• 批准号: 09671296
• 负责人: TANAKA Tatsuo
• 金额: $ 1.98万
• 依托单位: Hamamatsu University School of Medicine
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 1998
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09671296/
• 关键词: Immunophotodetection; Human Colon Cancer; ICCD Camera; Micro metastasis; CEA; Cy5.5; 近赤外線; 大腸癌; ガン転移; ヌードマウス; ヒトガン転移モデル

PBS, Ig G labeled with FITC, or monoclonal antibody labeled with FITC were injected i.v. into nude mice bearing s.c. xenografts of human colon carcinoma TK15. Fluorescent intensity of tumor tissue in the control and Mab group were 480.5 and 848.4 at 24 hr after injection, and decreased at 48 hr after injection. Fluorescent dye : indocyanin Cy5.5 has an advantage over fluorescein in that it emits a more penetrating fluorescent red signal at 690 nm after excitation with a laser ray of 635 nm. PBS, Ig G labeled with Cy5.5, or Mab CEA labeled with Cy5.5 were injected i.v. into nude mice bearing S.C.Xenografts of human colon carcinoma TK4. Their fluorescence were observed by ICCD camera system. Fluorescent intensity of tumor tissue in control, IgG, and Mab group were 11, 25, and 44 at 24 hr after injection. Fluorescent intensity in Mab group increased significantly than the other groups. Para-aorta and inguinal lymph nodes of mice bearing i.p. xenograft of TK4 were observed by ICCD camera at 24 hr after injection Mab CEA labeled with Cy5.5 i.v.. fluorescence of metastatic lymph node was observed clearly. Such a demonstration opens the way to a new form of clinical imnmunophotodiagnosis in colon carcinoma.

将PBS、FITC标记的IG G或FITC标记的单克隆抗体静脉内注射到皮下接种了抗人IgG抗体的裸鼠中。人结肠癌TK 15的异种移植物。对照组和单克隆抗体组肿瘤组织的荧光强度在注射后24小时分别为480.5和848.4，在注射后48小时下降。荧光染料：吲哚蓝蛋白Cy5.5具有优于荧光素的优点，因为它在用635 nm的激光射线激发后在690 nm处发射更穿透的荧光红色信号。将PBS、用Cy5.5标记的IG G或用Cy5.5标记的Mab CEA静脉内注射到携带人结肠癌TK 4的S.C.异种移植物的裸鼠中。用ICCD摄像系统观察它们的荧光。注射后24小时，对照组、IgG组和Mab组肿瘤组织的荧光强度分别为11、25和44。单克隆抗体组荧光强度明显高于其他各组。在静脉内注射Cy5.5标记的Mab CEA后24小时，通过ICCD照相机观察携带腹膜内异种移植TK4的小鼠的主动脉旁和腹股沟淋巴结。转移淋巴结荧光清晰可见。这为结肠癌的临床免疫光诊断开辟了新的途径。