Analyses of metastasis related gene using in situ hybridization against gastric cancer

原位杂交分析胃癌转移相关基因

• 批准号: 09671333
• 负责人: ANZAI Haruyuki
• 金额: $ 1.79万
• 依托单位: Saitama Medical School
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 1998
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09671333/
• 关键词: Gastric cancer; in situ hybridization; Epidermal qrowth Factor; basic fibroblast qrowth factor; E-cadherin; Matrix Metalloproteinase; Proqnosis; recurrence; in situ hybridization; Matrix metalloprotinase 2; VEGF; IL8; 総合的進行程度; gastric cancer

Correlation between the metastasis related several genes expression and recurrence in patient with gastric cancer were studied using the in situ hybridization technique. The following 6 metastasis related genes were chosen. Epidermal growth factor receptor (EGFR) is related to growth for the tumor cells. Basic fibroblast growth factor(bFGF), vascular endotherial growth factor (VEGF), IL8 plays very important roles in angiogenesis. Matrix proteinase 2 (MMP2) and E-cadherin (ECD) were chosen as extracellular matrix proteinase and adhesion molecule, respectively. The optical densities of each cases against probe were determined by comparison with poly dT(20), which was set to 100 using Luzex-FS image analyzer.1. EGFR, bFGF, ECD, MMP2 were related to recurrence of gastric cancer. According to expression of EGFR, bFGF, ECD mRNA, we calculated hazard and score of each cases. More than ten of the score indicated possibility of recurrence is around 60%.2. MMP2 /ECD ratio (M/E ratio), which is strongly related to invasion, indicated 1.4 in recurrent group and 0.9 in disease free group. More than 1.0 in M/E ratio, 9 of 11 cases were recurrenced.3. MMP2 and ECD study were progressed to material 61 cases. Both gene expression show the possibility to identify the poor prognostic patients in same conclusive cases. Especially, M/E ratio was related to the conclusive stage and revealed us to identify the poor prognostic patient.4. Positive relationship between EGFR and bFGF, bFGF and MMP2 was observed. EGFR and ECD indicated inverse relationship.5. mRNA expression of VEGF, IL8 were very low and could not be studied.

采用原位杂交技术研究胃癌转移相关基因表达与复发的相关性。选择以下6个转移相关基因。表皮生长因子受体（EGFR）与肿瘤细胞的生长有关。碱性成纤维细胞生长因子(bFGF)、血管内皮生长因子(VEGF)、IL8在血管生成中发挥着非常重要的作用。基质蛋白酶2（MMP2）和E-钙粘蛋白（ECD）分别被选为细胞外基质蛋白酶和粘附分子。通过与使用Luzex-FS图像分析仪将聚dT(20)设置为100的比较来确定每种情况对探针的光密度。 1． EGFR、bFGF、ECD、MMP2与胃癌复发相关。根据EGFR、bFGF、ECD mRNA的表达情况，计算每个病例的危险度和评分。超过10分表明复发可能性在60%左右。2． MMP2/ECD比值(M/E比值)与侵袭密切相关，复发组为1.4，无病组为0.9。 M/E大于1.0，11例中有9例复发。 3． MMP2和ECD研究已进展到材料61例。两种基因表达都表明有可能在相同的结论性病例中识别出预后不良的患者。特别是，M/E比值与最终分期有关，有助于我们识别预后不良的患者。 4． EGFR与bFGF、bFGF与MMP2呈正相关。 EGFR与ECD呈反比关系。 5． VEGF、IL8 mRNA表达量很低，无法进行研究。

项目成果

Haruyuki Anzai: "Expression of metastasis-related genes in surgical specimens of human gastric cancer can predict disease recurrence"European Journal of Cancer. vol 34. 558-565 (1998)

Haruyuki Anzai：“人胃癌手术标本中转移相关基因的表达可以预测疾病复发”欧洲癌症杂志。