The role of tenascin family molecules in brain tumors

生腱蛋白家族分子在脑肿瘤中的作用

• 批准号: 09671421
• 负责人: KOJIMA Tadashi
• 金额: $ 0.77万
• 依托单位: Mie University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 1998
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09671421/
• 关键词: glioma; tenascin-C; tenascin-R; tenascin-X; migration; proliferation; neovascularization; テネイシン-C; テネイシン-R; テネイシン-X; 神経膠種

We studied the expression of tenascin-C (TN-C), tenascin-R (TN-R), and tenascin-X (TN-X) in human gliomas to clarify the functions of tenascin family molecules in brain tumors. The distribution of TN-X was often reciprocal to that of TN-C.Cultured glioblastoma cells express a glioma-mesenchymal extracellular matrix glycoproteins, shown to be identical to TN-C, the degree of expression being linked to tumor grade in human gliomas. TN-C was more strongly expressed in the intercellular spaces and in tumor vessels in high-grade gliomas. In contrast, TN-X was mainly localized in the perivascular stroma around tumor vessels. While TN-R expression in the intercellular spaces is greater in anaplastic astrocytomas (Grade III) than inglioblastomas (Grade IV), In situ hybridization demonstrated that TN-C was produced by glioma cells and endothelial cells, and TN-R was produced only by glioma cells. Therefore TN-C may play significant roles inmigration and proliferation of glioma cells, and neovascularization. TN-X may play different roles in neovascularization, TN-C may in proliferation of glioma cells.

我们研究了腱生蛋白-C（TN-C）、腱生蛋白-R（TN-R）和腱生蛋白-X（TN-X）在人脑胶质瘤中的表达，以阐明腱生蛋白家族分子在脑肿瘤中的功能。TN-X的分布通常与TN-C的分布相反。培养的胶质母细胞瘤细胞表达胶质瘤间充质细胞外基质糖蛋白，显示与TN-C相同，表达程度与人类胶质瘤的肿瘤分级相关。TN-C在高级别胶质瘤的细胞间隙和肿瘤血管中表达更强。TN-X主要定位于肿瘤血管周围的血管间质。虽然TN-R在间变性星形细胞瘤（III级）的细胞间隙中的表达高于胶质母细胞瘤（IV级），但原位杂交表明TN-C由胶质瘤细胞和内皮细胞产生，TN-R仅由胶质瘤细胞产生。因此TN-C可能在胶质瘤细胞的迁移、增殖和新生血管形成中起重要作用。TN-X可能在新生血管形成中发挥不同的作用，TN-C可能在胶质瘤细胞增殖中发挥作用。

项目成果

Koichi Hasegawa: "Differential expression of tenascin-C and tenasin-X in human astrocytomas" Acta Neuropathol. 93. 431-437 (1997)

长谷川浩一：“人星形细胞瘤中腱蛋白-C 和腱蛋白-X 的差异表达”《神经病理学报》。

Noriko Hanamura: "Expression of fibronectin and tenascin-C mRNA by myofibroblasts, vascalar cells and epithelial cells in human colon adenomos and carcinomas" Int.J.Cancer. 73. 10-15 (1997)

Noriko Hanamura：“人结肠腺瘤和癌中肌成纤维细胞、血管细胞和上皮细胞表达纤连蛋白和腱蛋白-C mRNA” Int.J.Cancer。

Toshimichi Yoshida: "Co-expression of tenascin and fibrunectin in epithelial and stromal cells of benign lesions and ductal carcinoinas in the human breast" JOURNAL OF PATHOLOGY. 182. 421-428 (1997)

Toshimichi Yoshida：“人类乳腺良性病变和导管癌的上皮细胞和基质细胞中腱蛋白和纤连蛋白的共表达”病理学杂志。

吉田利通: "テネイシンファミリー分子の構造と機能" 生体の科学. 48・4. 273-279 (1997)

吉田敏道：“生腱蛋白家族分子的结构和功能”生物科学48・4（1997）。

Koichi Hasegawa: "Differential expression of tenascin-C and tenascin-X in human astrocytoma" Acta Neuropathol. 93. 431-437 (1997)

长谷川浩一：“人星形细胞瘤中腱蛋白-C 和腱蛋白-X 的差异表达”《神经病理学报》。