Investigating the function of astrocytes during neurotrophin-induced synaptogenesis and synaptic plasticity

研究神经营养素诱导的突触发生和突触可塑性期间星形胶质细胞的功能

• 批准号: 82265935
• 负责人: Professorin Dr. Daniela C. Dieterich
• 金额: --
• 依托单位: Institut für Pharmakologie und Toxikologie
• 依托单位国家: 德国
• 项目类别: Priority Programmes
• 财政年份: 2008
• 资助国家: 德国
• 起止时间: 2007-12-31 至 2011-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/82265935?language=en
• 关键词: Investigating; function; astrocytes; during; neurotrophin

The emerging concept of the “Tripartite synapse” points to the importance of glia cells for neuronal function and development. Although it is well established that astrocytes are important for the formation and maintenance of synaptic contacts, sense neuronal activity and actively participate in homeostatic scaling, it is unclear if the astroglial proteome is as dynamic as the neuronal. In recent years neurotrophins have emerged as important modulators of synaptogenesis and synaptic plasticity influencing both neurons and astrocytes. Therefore, I herein aim to explore the neuron-astroglia relationship in the context of neurotrophin-induced synaptic plasticity by performing cell-type specific proteomic profiling in astroglial-neuronal co-cultures based on the recently developed technique BONCAT. In addition, the proposed glial proteome dynamics shall be examined by visualization of the contact sites between neurons and astrocytes in the co-culture system. This work will deepen our understanding of the molecular and systemic aspects of the neuron-glia partnership.

“三方突触”的新兴概念指出了神经胶质细胞对神经元功能和发育的重要性。尽管众所周知，星形胶质细胞对于突触接触的形成和维持、感知神经元活动并积极参与稳态缩放很重要，但尚不清楚星形胶质细胞蛋白质组是否与神经元一样动态。近年来，神经营养素已成为影响神经元和星形胶质细胞的突触发生和突触可塑性的重要调节剂。因此，我在此旨在通过基于最近开发的 BONCAT 技术在星形胶质细胞 - 神经元共培养物中进行细胞类型特异性蛋白质组分析，探索神经营养蛋白诱导的突触可塑性背景下的神经元 - 星形胶质细胞关系。此外，应通过共培养系统中神经元和星形胶质细胞之间接触位点的可视化来检查所提出的神经胶质蛋白质组动力学。这项工作将加深我们对神经元-胶质细胞伙伴关系的分子和系统方面的理解。