Pregnancy diagnosis based on the fecal oestrone sulphate concentrations using an enzymeimmunoassay in the Mare

本研究描述了一种粪便硫酸雌酮（OS）的酶免疫测定法（EIA），该方法可以使母马怀孕，并从交配后约100天开始监测。从母马的新鲜粪便样本中提取硫酸雌酮，在室温下用甲醇摇晃过夜。等分甲醇提取物进行EIA，使用抗原包被检测格式和硫酸雌酮单克隆抗体。采用lug/well抗原，第一抗体1/20,000稀释，第二抗体1/1,000稀释，得到理想标准曲线。OS BIA的灵敏度<5pg/ml。随着妊娠的进展，OS的平均浓度逐渐升高。从交配后4个月开始，怀孕母马粪便中的OS浓度显著高于未怀孕母马。未怀孕的母马可以在大约4个月后被识别出来。

The present study describes an euzymeimmunoassay (EIA) for fecal oestrone sulphate (OS) which enables pregnancy in mares and monitored from approximately 100 days onwards following matingOestrone sulphate was extracted from fresh fecal samples in mares, by shaking in mnethanol for overnight at room temperature. Aliquots of the methanol extracts were subjected to EIA, using an antigen-coated assay format and monoclonal antibody to oestrone sulphate.The ideal standard curve was derived using lug/well antigen, 1/20,000 dilution of first antibody and 1/1,000 dilution of second antibody. The sensitivity of the OS BIA was <5pg/ml. The mean OS concentrations rose progressively as pregnancy advanced. From 4 months after mating onwards, the fecal OS concentrations of samples from pregnant mares exceeded significantly the values of non-pregnant mares. Non-pregnant mares can be identified from approximately 4 months postmating onwards.