Roles of activation of antigen specific cytoxic T cells against micro bial infections.

已经在几种动物模型中报道了使用pLasmid DNA编码的微生物抗原来提高针对病原体的体液和细胞免疫应答的功效，并且这种方法被称为DNA疫苗接种。在艾滋病疫苗开发领域，我们和其他人已经成功地通过DNA疫苗接种诱导了HIV-1特异性免疫应答。与传统的蛋白质疫苗相比，DNA疫苗在稳定性、易于生产和诱导细胞介导的免疫等方面具有潜在的优势。为了获得更强的免疫应答，人们尝试了一些方法，如利用基因枪系统的粒子轰击，或应用局部麻醉剂促进DNA进入肌肉细胞，并取得了实质性的成功。在本系列的一系列研究中，我们证明了1）DNA疫苗可以诱导高水平的Th 1免疫应答，2）并且这些应答通过添加IL-12表达质粒而增强。3)GM-CSF表达质粒增强Th 2型免疫应答。4)鼻内给药（i.n.）DNA疫苗比肌肉注射疫苗诱导的Th 2免疫应答水平更高。5)DNA疫苗可诱导小鼠产生高水平的粘膜免疫。本系列研究表明，DNA疫苗能诱导较好的抗原特异性免疫应答。

The efficacy of using pLasmid DNA-encoding microbial antigens to by us raise humoral and cellular immune responses against pathogens has been reported in several animal models, and this method is referred to as DNA vaccination. In the field of AIDS vaccine development, others and we have succeeded in inducing HIV-1-specific immune responses by DNA vaccination. DNA vaccination offers potential advantages over traditional protein-based vaccines in areas such as stability, ease of production, and induction of cell-mediated immunity. To obtain stronger responses, approaches such as the use of particle bombardment by means of a gene gun system, or application of local anesthetics to facilitate DNA entry into muscle cells have been attempted and have met with substantial success.In the present series of series of studies we have demonstrated that 1) DNA vaccine can induce a high level of Th1 immune responses, 2) and these responses are enhanced by the addition of IL-12 expression plasmids. 3) GM-CSF expression plasmids enhanced Th2 type immune responses. 4) Intranasal administration (i.n.) of DNA vaccine enhance induced higher levels of Th2 immune responses than intramuscular immunization. 5) In administration of DNA vaccine induced high levels of mucosal immunities. In this series of our studies revealed that DNA vaccine could induce a good level of antigen specific immune responses.