A Novel Type of Cell Death of Lymphocytes Induced by mAb

我们已经建立了一种大鼠抗小鼠单抗RE2，它能识别MHC I类单态区上的一个表位。RE2 mAb在缺乏补体的情况下杀死活化的淋巴细胞和白血病细胞系。电镜观察发现，re2处理后的细胞细胞膜上形成了巨孔。细胞溶解过程中未见DNA断裂和线粒体肿胀，提示细胞死亡不是凋亡或典型坏死。我们研究了RE2 mAb对自身免疫性疾病易发的NZB/W Fl小鼠淋巴细胞的影响。体内和体外用RE2处理后，腹腔和脾脏的BI细胞均被彻底清除。在另一项实验中，我们也研究了RE2 mAb对Con A诱导肝炎的作用。RE2完全抑制肝炎小鼠血清GOT和GPT的升高。RE2 mAb仅能杀死活化的淋巴细胞而非静止细胞，它可能有助于我们开发一种治疗自身免疫性疾病、暴发性肝炎和淋巴细胞白血病的治疗装置。

A Novel Type of Cell Death of Lymphocytes Induced by mAbWe have established a rat anti-mouse mAb, RE2, which recognizes an epitope that is on an MHC class I monomorphic region. RE2 mAb killed activated lymphocytes and leukemic cell lines in the absence of complement. Electromicroscopically, formation of Giant holes was found on the cell membrane of RE2-treated cells. There was no indication of DNA fragmentation nor swelling of mitochondria during the cell lysis, suggesting that the cell death is not due to apoptosis or typical necrosis. We investigated the effect of RE2 mAb on lymphocytes from autoimmune disease prone NZB/W Fl mice. BI cells in the peritoneal cavity and spleen were drastically eliminated by treatment with RE2 both in vivo and in vitro. In the other experiment, We also investigated the effect of RE2 mAb on Con A induced hepatitis. RE2 completely inhibited the elevation of serum GOT and GPT of the hepatitis induced mouse. RE2 mAb kills only activated lymphocytes but resting cells, it may help us to develop a therapeutic device for autoimmune diseases, Fulminant Hepatitis and lymphom as.