Analysis of the mechanism of chromosome-DNA length polymorphism and aplication to the gene amplification in yeast.

染色体-DNA长度多态性机制分析及其在酵母基因扩增中的应用

• 批准号: 09650884
• 负责人: TSUBOI Michio
• 金额: $ 1.54万
• 依托单位: Fukuyama University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 1998
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09650884/
• 关键词: yeast; Sacchromyces cerevisiae; chromosome III; chromosome DNA polymorphism; gene amplification; 遺伝子増幅; S.cerevisiae; S. cerevisiae; α-アミラーゼ

The length of chromosome III DNA of the strain of Saccharomyces cerevisiae, MT98a-3D, is 450 kbp, in contrast with the value of 350 kbp of ordinary strains. The DNA-length polymorophism of chromosome-III occur at a high frequency in spore clones derived from this strain. Restriction enzyme analyses and genetic analyses showed that the 450 kbp chromosome III DNA contains an excessive DNA fragment of about 100 kbp, on which the region from THR4 gene to HMR gene are located. It is indicated that the polymorphism is generated by unequal crossing over(s) at duplicated DNA sequences between sister chroinatids at meiosis. We planed to use this chromosome-DNA length polymorphism to the amplification of usefull genes on the chromosome in yeast.

酿酒酵母菌株的染色体III DNA的长度，mt98a-3d，是450 kbp，与普通菌株的350 kbp值相反。染色体-III的DNA长度多性友善发生在源自该菌株的孢子克隆中。限制酶分析和遗传分析表明，450 kbp III DNA含有大约100 kbp的过度DNA片段，从THR4基因到HMR基因的区域都位于该区域。据表明，多态性是通过在减数分裂的姐妹chroinatids之间的重复的DNA序列上越过不平等的交叉来产生的。我们计划使用这种染色体-DNA长度多态性来扩增酵母中染色体上有用基因。

项目成果

Taisuke Hisatomi: "The autonomously repllcating sequence(ARS)of the yeast Saccharomyces exiguus Yp74L・3." Current Microbiology. 38. 122-125 (1999)

Taisuke Hisatomi：“酵母酵母 Yp74L·3 的自主复制序列（ARS）。”《当代微生物学》38. 122-125（1999）。

Taisuke Hisatomi: "DNA sequence of the β isopropylmalate dehydrogenase gene and phylogenetic analysis of the yeast Saccharomyces exiguus Yp74L・3." Journal of Bioscience and Bioengineering. 87・1. 15-18 (1999)

Taisuke Hisatomi：“酵母酵母 Yp74L·3 的 β 异丙基苹果酸脱氢酶基因的 DNA 序列和系统发育分析。”《生物科学与生物工程杂志》87·1（1999 年）。

Taisuke Hisatomi: "DNA sequence of the β・isopropylmalate dehydrogenase gene and phylogenetic analysis of the yeast Saccharomyces exiguus Yp74L・3." Journal of Bioscience and Bioengineering. 87・1. 15-18 (1999)

Taisuke Hisatomi：“酵母酵母Yp74L·3的β·异丙基苹果酸脱氢酶基因的DNA序列和系统发育分析。”《生物科学与生物工程杂志》87·1（1999）。

Taisuke Hisatomi: "A short from the LEU2 gene of Saccharomyces cerevisiae functions as an ARS in the yeast Saccharomvces exiguus Yp74L・3." Current Microbiology. 37. 426-430 (1998)

Taisuke Hisatomi：“酿酒酵母 LEU2 基因的一个短片段在酵母 Saccharomyces exiguus Yp74L·3 中充当 ARS。”《当代微生物学》37. 426-430 (1998)。

Taisuke Hisatomi: "Identification of the orotidine・5' phosphate decarboxylase gene and development of a transformation system in the yeast Saccharomyces exiguus Yp74L・3." Bioscience,Biotechnology and Biochemistry. 62・11. 2280-2282 (1998)

Taisuke Hisatomi：“酵母中乳清苷·5磷酸脱羧酶基因的鉴定和转化系统的开发。生物科学、生物技术和生物化学62·11。”