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Study on substances regulating pollen germination in Japanese chestnut

板栗花粉萌发调节物质的研究

基本信息

批准号：
09660038
负责人：
NAKAMURA Masahiro
金额：
$ 2.11万
依托单位：
Miyagi Agricultural College
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1997
资助国家：
日本
起止时间：
1997 至 1999
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09660038/
关键词：
repressive substances of pollen germination promotive substances of pollen germination solution extracted with water from styles pollen germination on stigma development of ovules substances regulating pollen germination shikimic acid sugar

项目摘要

The study was conducted to clarify the substances regulating pollen germination and pollen tube growth in the styles of Japanese chestnut (Castanea crenata Sieb. Et Zucc.) in concerned with development of ovules. Pollen germination and pollen tube growth on the stigma began after the style ceased to grow, although the anthesis of male flowers had already began. The solution extracted with water from the styles at early stage of the growth repressed remarkably pollen germination and pollen tube growth, although its repressive effect disappeared as the style ceased the growth. The HPLC analysis of the extracted solution showed that repressive substance might be shikimic acid, as estimated from retention time. Sugar content in the extracted solution was higher both at early stage of the style growth and at the beginning of pollen germination and pollen tube growth on the stigma. When pollen tubes began to grow on the stigma, the development of ovules was at early stage in which no nucellus was formed in each ovules in a ovary. The HPLC analysis of the extracted solution under different analytical condition showed the possibility of existence of both repressive and promotive substances which were produced by styles before and after pollen germination and pollen tube growth began on the stigma, respectively. However, no distinct relationship between the production of those substances and the development of ovules was indicated. Therefore, it was concluded that pollen germination and pollen tube growth on the stigma were regulated by the substances produced from stigma and style themselves.

本研究旨在阐明板栗(Castanea crenata Sieb.)花柱中控制花粉萌发和花粉管生长的物质。Et Zucc.)与胚珠的发育有关。虽然雄花的开花已经开始，但柱头上的花粉萌发和花粉管生长是在花柱停止生长后开始的。花柱早期用水提取液对花粉萌发和花粉管生长有明显抑制作用，但随着花柱停止生长，抑制作用消失。提取液的高效液相色谱分析表明，根据保留时间估算，抑制物质可能是莽草酸。无论是在花柱生长初期，还是在柱头上的花粉萌发和花粉管生长初期，提取液的含糖量都较高。当柱头上的花粉管开始生长时，胚珠的发育处于早期阶段，子房中的每个胚珠都没有形成珠心。不同分析条件下提取液的高效液相分析表明，柱头上分别存在花柱在花粉萌发和花粉管生长前后产生的抑制物质和促进物质。然而，这些物质的产生与胚珠的发育之间没有明显的关系。因此，柱头上的花粉萌发和花粉管生长受柱头和花柱自身产生的物质调控。