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Study on the light-protecting effect of polyacylated anthocyanins

多酰化花青素的避光作用研究

基本信息

批准号：
09660147
负责人：
KAMEDA Kiyoshi
金额：
$ 2.05万
依托单位：
Sugiyama Jogakuen University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1997
资助国家：
日本
起止时间：
1997 至 1999
项目状态：
已结题

项目摘要

The antohcyanin is the natural colorant which is not only a safe food-colorant but also is expected antioxidant, cancer inhibition, and immunostimulating effect and, etc. In the meantime, ultraviolet ray protecting function can be estimated because the genetic hazard does not occur, even if the flower, a genital organ, blooms under the solar light for a long period. This function is very interesting for basic science study, and in addition, it is important from the viewpoint of the development of the highly functional food-coloring agent. For this purpose I studied on stability of heavenly blue anthocyanin (HBA) from Ipomoea tricolor and protection of DNA damage.1. Light irradiating experiment using the Okazaki Large Spectrograph (OLS).HBA was dissolved in 0.5% TFA-methanol solution, or pH4, 6, and pH 7.5 buffer solution, then using the OLS, monochromatic UV light from 280 nm to 400 nm was irradiated. Under the physiological condition, HBA was the most stable, and in acidic methanol the most unstable. The pigment decomposed more rapidly at 280 nm than 370 nm.2. A protection of DNA damage by the pigment.HBA and bovine thymus DNA was dissolved in pH7.5 buffer solution, and then the solution was irradiated by ultraviolet radiation of UV-B region. The degree of a DNA damage was determined quantitatively by the ELISA in the cooperation of Professor Nikaido of the Kanazawa University. HBA exhibited the protecting effect against degradation of DNA by UV-B irradiation.

花青素是一种天然色素，不仅是一种安全的食品着色剂，而且具有抗氧化、抑癌、免疫刺激等作用。同时，作为生殖器官的花，即使在阳光下长时间开花，也不会发生遗传危害，因此可以估计其紫外线防护功能。这一功能不仅对基础科学研究具有重要意义，而且对开发高功能食品着色剂具有重要意义。为此，我研究了三色益马天蓝花青素（HBA）的稳定性及对DNA损伤的保护作用。冈崎大型摄谱仪（OLS）的光照射实验。将HBA溶解于0.5% tfa -甲醇溶液，或pH4、6、pH 7.5缓冲溶液中，然后使用OLS， 280 ~ 400 nm单色紫外光照射。生理条件下HBA最稳定，在酸性甲醇中最不稳定。色素在280 nm处比370 nm处分解更快。一种保护DNA损伤的色素。将HBA和牛胸腺DNA溶解在pH7.5的缓冲液中，然后用UV-B区紫外线照射。在金泽大学二阶堂教授的合作下，用ELISA定量测定了DNA损伤程度。HBA对UV-B照射下DNA的降解具有保护作用。