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MOLECULAR APPROACH TO THE MICROBIAL POPULATION IN ACTIVATED SLUDGE IN RELATION WITH THE PERFORMANCE OF WASTEWATER TREATMENT PLANTS

活性污泥中微生物种群与废水处理厂性能关系的分子方法

基本信息

批准号：
09650599
负责人：
MINO Takashi
金额：
$ 2.5万
依托单位：
THE UNIVERSITY OF TOKYO
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1997
资助国家：
日本
起止时间：
1997 至 1998
项目状态：
已结题

项目摘要

The goal of the wastewater treatment used to be the removal of BOD, but nowadays, the removal of nitrogen and phosphorus is required. As the required level of treatment gets higher, we need to understand he microbial population and to make the best of microbial power to remove pollutants. In this research, we employed the fluorescence DNA probes which enables the analysis of microbial population structure for the analysis of microbial population in activated sludge in wastewater treatment plants. One of the important points about using the fluorescent DNA probes is that microbial population can be estimated without cultivation.In this research, we analyzed the activated sludge in 20 treatment lines in 15 wastewater treatment plants in Hokkaido, Tokyo, Saitama, and Okinawa areas. Four surveys were conducted on each of the treatment plants ; November 1997, January, Nay, and August 1998. Six probes were used for the investigation which are targeted at (l)eubacteria, (2) Proteobacteria alpha-subclass, (3) Proteobacteria beta-subclass, (4) Proteobacteria gamma-subclass, (5) Gram positive GC high group, and (6) Cytophaga-Flavobacterium group.It was demonstrated that in many of the wastewater plants, Proteobacteria (alpha- + beta- + gamma-subclasses) were the predominant group of eubacteria, and that alpha-subclass and the beta-subclass are the most predominant in the Proteobacteria. No significant relationships were found between the microbial population structure and the conditions of the wastewater treatment plants, such as location, water temperature, and the nutrient removal. But it was found that the smaller wastewater treatment plants contain more eubacteria which were not detected by the group-specific probes used in this study. In many of the wastewater treatment plants, increase in the alpha-subclass and the decrease in the Gram positive GC high group from winter to summer were observed.

过去废水处理的目标是去除BOD，而现在则要求去除氮和磷。随着所需的处理水平越来越高，我们需要了解微生物种群，并充分利用微生物的力量来去除污染物。在本研究中，我们采用荧光DNA探针，使微生物种群结构的分析，用于分析在污水处理厂的活性污泥中的微生物种群。关于使用荧光DNA探针的重要点之一是可以在没有培养的情况下估计微生物种群。在本研究中，我们分析了北海道，东京，琦玉和冲绳地区的15个污水处理厂的20条处理线的活性污泥。对每个处理厂进行了四次调查; 1997年11月，1998年1月，Nay和8月。使用六种探针进行研究，其针对（1）真细菌、（2）变形菌门α-亚类、（3）变形菌门β-亚类、（4）变形菌门γ-亚类、（5）革兰氏阳性GC高组和（6）噬细胞黄杆菌组。（α- + β- + γ-亚类）是真细菌的主要类群，并且α-亚类和β-亚类在变形菌门中是最主要的。微生物种群结构与污水处理厂的条件，如位置，水温和营养物去除之间没有显着的关系。但发现较小的污水处理厂含有更多的真细菌，而本研究中使用的组特异性探针未检测到。在许多污水处理厂，从冬季到夏季的α-亚类和减少革兰氏阳性GC高组的增加进行了观察。