Investigation on diversity in the structure of cellulose-synthesizing terminal complexes among algal groups possessing chlorophyll a and c.

具有叶绿素a和c的藻类纤维素合成末端复合物结构多样性的研究。

• 批准号: 09640828
• 负责人: OKUDA Kazuo
• 金额: $ 1.86万
• 依托单位: Kochi University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 1999
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09640828/
• 关键词: Chlorophyll a and c; Cellulose microfibril; Cellulose-synthesizing terminal complex; diversity; Freeze fracture electron microscopy; クロロフィルa+c植物; セルロース; 細胞外被; 凍結割断法

The structure of cellulose microfibrils (CMFs) and cellulose-synthesizing terminal complexes (TCs) in some species with chlorophyll a and c such as phaeophycean, xanthophycean and dinophycean algae was examined by freeze fracture electron microscopy. Both of Sphacelaria rigidula (Phaeophyceae) and Botridium sp. (Xanthophyceae) synthesized ribbon-like CMFs. However, Sphacelaria had TCs whose subunits are arranged in a single linear row, whereas TCs in Botridium consisted of several diagonal rows of subunits. It was found first that alginates are present in Xanthophyceae besides in Phaeophyceae, indicating a phylogenetically close relationship between Xanthophyceae and Phaeophyceae. These results suggest that the TCs in both or either of - Xanthophyceae and/or Phaeophyceae had been acquired at divergence of these two clades. It was evident in the dinophycean Scrippsiella hexapraecingra that the thecal plates contain CMFs which form bundles consisting of several fine components by electron diffraction methods. A new TC was found in this alga.

用冷冻断裂电镜技术研究了几种具有叶绿素a和叶绿素c的藻类（褐藻、黄藻和甲藻）的纤维素微纤丝（CMFs）和纤维素合成末端复合物（TC）的结构。硬顶黑球藻（Sphacelaria rigidula）和黄藻（Botridium sp.）均能合成带状CMFs。然而，Sphacelaria的TC的亚基排列在一个单一的线性行，而在Botridium TC由几个对角行的亚基。首次发现褐藻酸盐在褐藻纲以外的黄藻纲中也存在，表明黄藻纲与褐藻纲在亲缘关系上较为密切。这些结果表明，在这两个分支的分歧，黄藻和/或褐藻中的两个或任何一个的TC已获得。通过电子衍射方法可以明显看出，甲藻斯氏藻六脉藻的鞘板含有CMF，这些CMF形成由几种精细成分组成的束。在该菌中发现了一个新的TC。

项目成果

Chi E-S.et al.: "Immunolgold-labeling analysis of alginate distributions in the cell walls of chromophyte algae"Phycological Research. 47. 53-60 (1999)

Chi E-S.等人：“有色藻类细胞壁中藻酸盐分布的免疫金标记分析”藻类研究。

K Okuda,I Mine,T Morinaga,K Kuwaki: "Cytomorphogenesis in coenocytic green algae.V.Segregative cell division and cortical microtubules in Dictyosphaeria cavernose(Siphonocladales,Chlorophyceae)." Phycological Research. 45. 189-196 (1997)

K Okuda、I Mine、T Morinaga、K Kuwaki：“共生绿藻的细胞形态发生。V.海绵状网腔藻（管藻目、绿藻纲）中的分离细胞分裂和皮质微管。”

Okuda K & Mine l: "Biosynthesis of algal cellulose"Cellulose communications. 4. 101-107 (1997)

奥田K

S Sekida,T Horiguchi, K Okuda: "Direct evidence for cellulose microfibrils present in thecal plates of the dinoflagellate Scrippsiella hexapraecingula." Hikobia. 13 in press. inpress (1999)

S Sekida、T Horiguchi、K Okuda：“甲藻 Scrippsiella hexapraecingula 鞘板中存在纤维素微纤维的直接证据。”

奥田一雄・峯一朗: "藻類セルロースの生合成" Cellulose Comunications. 4・3. 101-107 (1997)

奥田一夫和峰一郎：“藻类纤维素的生物合成”纤维素通讯 4·3（1997）。