Medical application of microencapsulating hybridoma cells in agarose microbeads "Cytomedicine"

有人建议将活细胞或组织微囊化，以防止移植后它们的免疫破坏。在这项研究中，我们通过细胞毒试验检测了微囊化的海藻酸盐-聚(L)赖氨酸-海藻酸膜中的SK2杂交瘤细胞(APA-SK2细胞)是否从同种异体宿主的免疫系统中免疫分离。APA膜抑制宿主细胞免疫反应的激活，但不能阻止同种异体移植后针对被包裹的SK2细胞的细胞毒抗体的产生。然而，APA-SK2细胞在SK2细胞免疫的小鼠和完整小鼠中仍然具有生命力。我们认为，由于APA-SK2细胞被兔抗SK2细胞抗血清破坏，存在于细胞膜上的补体调节因子具有种属特异性限制，从而阻断了补体介导的细胞裂解作用。结果表明，APA膜能抑制被包裹的细胞与宿主淋巴细胞之间的细胞接触，但不能完全保护被包裹的细胞免受异种人的免疫。

Microencapsulation of living cells or tissues has been proposed to prevent their immune destruction following transplantation. In this study, we examined whether SK2 hybridoma cells microencapsulated in an alginate-poly(L)lysine-alginate (APA) membrane (APA-SK2 cells) were immunoisolated from the allogeneic host's immune system using a cytotoxicity test. The APA membrane inhibited the activation of the host's cellular immune response, but did not prevent the production of cytotoxic antibodies against entrapped SK2 cells following allogeneic transplantation. However, the APA-SK2 cells remained vital in SK2 cell-immunized mice as well as in intact mice. We considered that complement regulatory factors which were present on cell membrane and had species-specific restriction blocked the complement-mediated cell lysis on allogeneic transplantation, since APA-SK2 cells were destroyed by rabbit anti-SK2 cell antiserum. Our results demonstrated that APA membrane could inhibit cell-cell contact between entrapped cells and the host's lymphocytes, but could not completely protect the entrapped cells from xenogeneic hamoral immunity.