Medical application of microencapsulating hybridoma cells in agarose microbeads "Cytomedicine"

已提出将活细胞或组织微胶囊化以防止移植后的免疫破坏。在这项研究中，我们通过细胞毒性试验，检测了微包裹在海藻酸-聚赖氨酸-海藻酸盐（APA）膜上的SK2杂交瘤细胞（APA-SK2细胞）是否从异体宿主的免疫系统中被免疫分离出来。APA膜抑制宿主细胞免疫反应的激活，但不阻止异体移植后对被包裹的SK2细胞产生细胞毒性抗体。然而，APA-SK2细胞在SK2细胞免疫小鼠和完整小鼠中仍然至关重要。我们认为，补体调节因子存在于细胞膜上，具有物种特异性限制，阻断了补体介导的异体移植细胞裂解，因为APA-SK2细胞被兔抗sk2细胞抗血清破坏。我们的研究结果表明，APA膜可以抑制被困细胞与宿主淋巴细胞之间的细胞间接触，但不能完全保护被困细胞免受异种免疫损伤。

Microencapsulation of living cells or tissues has been proposed to prevent their immune destruction following transplantation. In this study, we examined whether SK2 hybridoma cells microencapsulated in an alginate-poly(L)lysine-alginate (APA) membrane (APA-SK2 cells) were immunoisolated from the allogeneic host's immune system using a cytotoxicity test. The APA membrane inhibited the activation of the host's cellular immune response, but did not prevent the production of cytotoxic antibodies against entrapped SK2 cells following allogeneic transplantation. However, the APA-SK2 cells remained vital in SK2 cell-immunized mice as well as in intact mice. We considered that complement regulatory factors which were present on cell membrane and had species-specific restriction blocked the complement-mediated cell lysis on allogeneic transplantation, since APA-SK2 cells were destroyed by rabbit anti-SK2 cell antiserum. Our results demonstrated that APA membrane could inhibit cell-cell contact between entrapped cells and the host's lymphocytes, but could not completely protect the entrapped cells from xenogeneic hamoral immunity.