Medical application of microencapsulating hybridoma cells in agarose microbeads "Cytomedicine"

琼脂糖微珠中微囊化杂交瘤细胞的医学应用“细胞医学”

• 批准号: 09557202
• 负责人: TSUTSUMI Yasuo
• 金额: $ 4.16万
• 依托单位: Osaka University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 1998
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09557202/
• 关键词: Cytomedicine; APA-microcapsule; Immunoisolation; Drug Delivery System

Microencapsulation of living cells or tissues has been proposed to prevent their immune destruction following transplantation. In this study, we examined whether SK2 hybridoma cells microencapsulated in an alginate-poly(L)lysine-alginate (APA) membrane (APA-SK2 cells) were immunoisolated from the allogeneic host's immune system using a cytotoxicity test. The APA membrane inhibited the activation of the host's cellular immune response, but did not prevent the production of cytotoxic antibodies against entrapped SK2 cells following allogeneic transplantation. However, the APA-SK2 cells remained vital in SK2 cell-immunized mice as well as in intact mice. We considered that complement regulatory factors which were present on cell membrane and had species-specific restriction blocked the complement-mediated cell lysis on allogeneic transplantation, since APA-SK2 cells were destroyed by rabbit anti-SK2 cell antiserum. Our results demonstrated that APA membrane could inhibit cell-cell contact between entrapped cells and the host's lymphocytes, but could not completely protect the entrapped cells from xenogeneic hamoral immunity.

已经提出活细胞或组织的微囊化以防止它们在移植后的免疫破坏。在这项研究中，我们检查是否SK2杂交瘤细胞微囊化在海藻酸盐-聚（L）赖氨酸-海藻酸盐（阿帕）膜（APA-SK2细胞）免疫隔离从同种异体宿主的免疫系统使用细胞毒性试验。阿帕膜抑制宿主细胞免疫反应的激活，但不能阻止异基因移植后针对截留的SK2细胞的细胞毒性抗体的产生。然而，APA-SK2细胞在SK2细胞免疫的小鼠以及完整的小鼠中仍然是有生命力的。我们认为，存在于细胞膜上的具有种特异性限制的补体调节因子阻断了同种异体移植中补体介导的细胞溶解，因为兔抗SK2细胞抗血清破坏了APA-SK2细胞。结果表明，阿帕膜能抑制包埋细胞与宿主淋巴细胞的细胞接触，但不能完全保护包埋细胞免受异种免疫的侵害。

项目成果

Tsutsumi Y.et al.: "Immunological studies of SK2 hybridoma cells microencapsulated with alginate-poly(L)lysine-alginate (APA)membrane following allogeneic transplantation." Biochem.Biophys.Res.Commun.230. 524-527 (1998)

Tsutsumi Y.等人：“同种异体移植后用藻酸盐-聚（L）赖氨酸-藻酸盐（APA）膜微囊化的 SK2 杂交瘤细胞的免疫学研究。”

Tsutsumi Y.et al.: "Tumor necrosis factor alpha-mediated tumor regression by the in vivo transfer of genes in to the artery that leads to tumors." Cancer Res.58. 5725-5730 (1998)

Tsutsumi Y.等人：“通过体内将基因转移到导致肿瘤的动脉中，肿瘤坏死因子α介导的肿瘤消退。”

Mayumi T.et al.: "The optimization of cytoplasmic gene expression system with T7 RNA polymerase." Drug Delivery System. 13. 87-93 (1998)

Mayumi T.et al.：“利用 T7 RNA 聚合酶优化细胞质基因表达系统。”

Mayumi T.et al.: "Development of novel drug delivery system of bioactive molecules from ″Cytomedicine″ using hybridoma cells entrapped in APA microcapsules." Drug Delivery System. 12. 107-114 (1997)

Mayumi T. 等人：“利用 APA 微胶囊中的杂交瘤细胞开发‘细胞医学’新型生物活性分子药物输送系统。”12. 107-114 (1997)。

Mayumi T.et al.: "Possibility of cytomedical therapy for diabetes melitus using microcapsulated pancreatic β cell line with glucose sensor." Drug Delivery System. 13. 95-100 (1998)

Mayumi T. 等人：“使用带有葡萄糖传感器的微囊胰腺 β 细胞系进行细胞医学治疗的可能性。”13. 95-100 (1998)。