In vivo application of polySia-based materials in a rat model of peripheral nerve regeneration - Impact on morphological and functional regeneration

PolySia 基材料在大鼠周围神经再生模型中的体内应用 - 对形态和功能再生的影响

• 批准号: 86996325
• 负责人: Professorin Dr. Claudia Grothe
• 金额: --
• 依托单位: Institut für Neuroanatomie und Zellbiologie
• 依托单位国家: 德国
• 项目类别: Research Units
• 财政年份: 2008
• 资助国家: 德国
• 起止时间: 2007-12-31 至 2011-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/86996325?language=en
• 关键词: vivo; application; polySia; based; materials

Regeneration of peripheral nerves depends crucially on the length of the gap between transected nerve stumps. To replace autologous nerve grafts, the current clinical golden standard, artificial nerve interponates containing factors that instruct and promote nerve regeneration are needed in the future. To optimally fulfil their functions, these artifical growth supports should provide the information in a spatial and temporal pattern and should be degraded while the autologeous nerve is build. Here we focus on the use of polySia-based materials to build this kind of guides. PolySia naturally involved in nerve regeneration and in the first period shown to drastically enhance sciatic nerve regeneration if exogenously added, will, in the course of this project, be applied in elaborated forms like e.g. nanofibers with specific, diameter, surface texture and instructive decoration. Therefore, neurostimulatory extracellular matrix proteins (e.g. laminin-1) and neurotrophic factors (e.g. FGF-2) will be either covalently bound to or encapsulated in the scaffold materials according to protocols developed in WP2.2 - WP2.5. The success of nerve regeneration will be studied with regard to amount and quality of regenerated nerve fibres, behavioural and electrophysiological recovery, systemic and local immunological status. Finally, identification of transplanted genetically modified cells within the regenerated tissue will be performed.

周围神经的再生关键取决于被切断的神经残端之间的间隙的长度。为了取代目前的临床黄金标准--自体神经移植，未来需要含有指导和促进神经再生的因子的人工神经间隔物。为了以最佳方式履行它们的功能，这些人造的生长支持应该提供时空模式的信息，并且应该在构建自体神经的同时被降解。在这里，我们重点介绍如何使用基于PolySia的材料来构建这种导轨。PolySia天然参与神经再生，在第一阶段显示，如果外源添加，可以极大地促进坐骨神经再生，在本项目过程中，将以精心设计的形式应用，例如具有特定直径、表面纹理和有指导意义的装饰的纳米纤维。因此，根据WP2.2-WP2.5制定的方案，神经刺激细胞外基质蛋白(例如层粘连蛋白-1)和神经营养因子(例如成纤维细胞生长因子-2)将被共价结合或包裹在支架材料中。神经再生的成功将从再生神经纤维的数量和质量、行为和电生理恢复、全身和局部免疫状态等方面进行研究。最后，将进行再生组织内移植的转基因细胞的鉴定。