In vivo application of polySia-based materials in a rat model of peripheral nerve regeneration - Impact on morphological and functional regeneration

周围神经的再生关键取决于切断的神经残端之间的差距的长度。为了取代目前临床上的金标准自体神经移植物，未来需要含有指导和促进神经再生因子的人工神经内插物。为了最佳地实现它们的功能，这些人工生长支持物应该以空间和时间模式提供信息，并且应该在构建自体神经时降解。在这里，我们专注于使用基于polySia的材料来构建这种指南。PolySia天然参与神经再生，并且在第一阶段显示，如果外源性添加，将大大增强坐骨神经再生，在该项目的过程中，将以精心制作的形式应用，例如具有特定直径，表面纹理和指导性装饰的纳米纤维。因此，根据WP2.2 - WP2.5中开发的方案，神经刺激性细胞外基质蛋白（例如层粘连蛋白-1）和神经营养因子（例如FGF-2）将共价结合或包封在支架材料中。将从再生神经纤维的数量和质量、行为和电生理恢复、全身和局部免疫状态方面研究神经再生的成功。最后，将进行再生组织内移植的遗传修饰细胞的鉴定。

Regeneration of peripheral nerves depends crucially on the length of the gap between transected nerve stumps. To replace autologous nerve grafts, the current clinical golden standard, artificial nerve interponates containing factors that instruct and promote nerve regeneration are needed in the future. To optimally fulfil their functions, these artifical growth supports should provide the information in a spatial and temporal pattern and should be degraded while the autologeous nerve is build. Here we focus on the use of polySia-based materials to build this kind of guides. PolySia naturally involved in nerve regeneration and in the first period shown to drastically enhance sciatic nerve regeneration if exogenously added, will, in the course of this project, be applied in elaborated forms like e.g. nanofibers with specific, diameter, surface texture and instructive decoration. Therefore, neurostimulatory extracellular matrix proteins (e.g. laminin-1) and neurotrophic factors (e.g. FGF-2) will be either covalently bound to or encapsulated in the scaffold materials according to protocols developed in WP2.2 - WP2.5. The success of nerve regeneration will be studied with regard to amount and quality of regenerated nerve fibres, behavioural and electrophysiological recovery, systemic and local immunological status. Finally, identification of transplanted genetically modified cells within the regenerated tissue will be performed.