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Refulation of PAF biosynthesis and its role in salivary secretion

Refulation of PAF biosynthesis and its role in salivary secretion
PAF生物合成的调节及其在唾液分泌中的作用
批准号:
09470403
负责人:
DOHI Toshihiro
金额:
$2.5万
依托单位:
依托单位国家:
日本
项目类别:
Grant-in-Aid for Scientific Research (B)
财政年份:
1997
资助国家:
日本
项目状态:
已结题
起止时间:
1997 至 1998

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中文摘要
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英文摘要
Platelet-Activating Factor (PAP) was released from a variety of inflammatory and immune-related cells. Moreover, PAF production has been demonstrated in a wider variety of cells and tissues, and suggested that PAF plays various physiological and pathological roles. We have previously shown that PAF is generated upon physiological stimulations in dog salivary gland cells. The present study examined the regulation of PAP biosynthesis and its role in secretory response in salivary glands.1 Acetylcholine (ACh), phenyleprine, isoproterenol and 8Br-cyclic AMP increased lyso-PAP : acetylCoA acetyltransferase (ACT) and DDT-insensiteve cholinephosphotransferase. ACh and phenylephrine increased ACT, and PAP generation in a Ca^2^+-dependent manner.2 PAP receptor expression in salivary glands was studied by examining PAF receptor gene expression using the RT-PCR technique. The specific amplification of PAP receptor-specific PCR fragment from guinea pig, but not dog salivary glands was detected.3 X … More enopus laevis oocytes injected with mRNA from submandibular glands of guinea pig and dogs were electrohysiologically analysed. Application of 1 muM PAP elicited a small current in the oocytes injected with mRNA from guinea pig but not dog submandibular glands.4 PAP stimulated mucin release in the slice of gunea pig submandibular glands.5 PAP is not releasable out of the cells but retained intracellulary in the cells. To assess its intracellular activity, the effect of PAP on Na^+, K^+-ATPase activity in the membrane fraction was examined. PAF inhibited Na^+, K^+-ATPase in the membrane preparations of dog submandibular glands. The inhibitory effect of a low concentration but not of high concentrations of PAP was antagonized by a PAP-receptor antagonists, BN 50739. Kinetic analysis of PAP-induced inhibition of Na^+, K^+-ATPase suggests that the inhibition of Na^+, K^+-ATPase by PAP is not due to competition by PAP at K^+-or Na^+-binding sites on the enzyme, but by complex inhibitory mechanisms.These results suggested that l : PAP biosynthesis is regulated autonomic nerve via remodelling pathway, 2 : PAP receptor is expressed guinea pig salivary gland and functions to stimulate secretory response, 3 : PAP may act as intracellular signal messenger to modulate secretory response in salivary glands. Less
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H.Yamaki et al.: "Cyclic ADP-ribose induces Ca^+ release from caffeine-insensitive Ca^<2+> pools in canine salivary glands." Journal of Dental Research. 77. 1807-1816 (1998)
H.Yamaki 等人:“环状 ADP-核糖诱导犬唾液腺中对咖啡因不敏感的 Ca^2 池中的 Ca^2 释放。”
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通讯作者:
K.Shibata et al.: "Platelet-activating factor in gingival crevicular fluid from periodontitis patients as a potent clinical disease activity marker." J.Int.Acad.Periodontol.(in press).
K.Shibata 等人:“牙周炎患者龈沟液中的血小板激活因子可作为有效的临床疾病活动标志物。”
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通讯作者:
Yamaki, H.et al.: "Cyclic ADP-ribose induces Ca^<2+> release from caffeine-insensitive Ca^<2+> pools in canine salivary gland." Journal of Dental Research. (in press). (1998)
Yamaki, H.et al.:“环状 ADP-核糖诱导犬唾液腺中对咖啡因不敏感的 Ca^2 池中的 Ca^2 释放。”
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    • 项目类别:
      Grant-in-Aid for Scientific Research (B).
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    • 项目类别:
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      --
    • 项目类别:
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    • 批准年份:
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