Pharmacological research on CaィイD12+ィエD1 channel abnormality which induces epileptic seizures.

The following studies were performed to elucidate the function of CaイイD12+イエD1。channels which induce epileptic seizures in spontaneously epileptic rat （SER： tm/tm， zi/zi）.1. Search for the gene to induce epileptic seizures in SER ：tm gene was found to locate in the area between D10M11Mit351 and D10M11Mit7 on the chromosome 10 and delate approximately 200 KB between the area。天冬氨酸酶基因，用于代谢化N-乙酰-L-天冬氨酸（NAA）的酶被发现包括在缺失中。实际上，NAA水平被增加在NAA诱导的absence样seizures中的脑和内切循环注射与5- 10 Hz spike-wave复合体中的Corpine和Hippocampal EEG在正常的Wistar大鼠和预成熟的Tremor大鼠中，其中包括SER的父母，以及暴露在该年龄之前的absence样seizures。NAA还在正常黄鼠狼率CA 3中诱导脱离极化转移和重复性first.2。Enhancement of CaイイD12+イエD1 influxes in SER hippocampus ：In the hippocampal slice preparation of SER which is exhibiting the seizures。CaイイD12+イエD1。在CA 3区域神经元与高K下治疗的Fura 2/AM比正常大鼠中的那些更多。

The following studies were performed to elucidate the function of CaィイD12+ィエD1. channels which induce epileptic seizures in spontaneously epileptic rat (SER: tm/tm, zi/zi).1. Search for the gene to induce epileptic seizures in SER :The tm gene was found to locate in the area between D10M11Mit351 and D10M11Mit7 on the chromosome 10 and delate approximately 200KB between the areas. The aspartoacetylase gene, the enzyme for metabolizing N-acetyl-L-aspartate (NAA) was found to be included within the deletion. Actually NAA level was increased in the brain and intra cerebroventricular injection of NAA induced absence-like seizures accompanying with 5-10Hz spike-wave complexes in cortical and hippocampal EEG in the normal wistar rat and the premature tremor rat, which is parent strain for SER and exhibits absence-like seizures before the age with the seizures. NAA also induced depolarization shift and repetitive firing in hippocampal CA3 of the normal wistar rat.2. Enhancement of CaィイD12+ィエD1 influxes in SER hippocampus :In the hippocampal slice preparation of SER which is exhibiting the seizures. CaィイD12+ィエD1. influxes in CA3 area neurons with high K' under treatment with fura 2/AM were more pronounced than those in the normal rat.