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Studies on the origin of the Japanese based on the analysis of MVR-PCR alleles in asian populations

基于亚洲人群MVR-PCR等位基因分析的日本人起源研究

基本信息

批准号：
09470123
负责人：
KATSUMATA Yoshinao
金额：
$ 6.14万
依托单位：
Nagoya University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
1997
资助国家：
日本
起止时间：
1997 至 1998
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09470123/
关键词：
Population Genetics Minisatellite D7S21 D1S8 D16S309 MVR-PCR Origin of the Japanese

项目摘要

Last year, we could obtain DNA specimens of 100 Chinese individuals, and investigated the allele frequencies of three polymorphic sites in DNA flanking D1S8 (MS32) and D7S21 (MS31A) loci. Haplotype frequencies in Chinese were compared with those in Japanese and Caucasians which had been already reported. Distribution of haplotypes in Chinese is not significantly different from that in Japanese, but significantly different from that in Caucasian. Allele-specific MVR-PCR based on the selective amplification of single allele from total genomic DNA using allele-specific PCR primers was then performed. Although MVR-PCR allele codes are highly polymorphic, the similarity of the internal structure can be analysed by dot matrix analysis. Furthermore, different alleles can have related flanking haplotypes, presumably due to sharing recent common ancestors. When groups of aligned MS32 alleles or MS31A alleles were made by dot matrix analysis, we have already shown that Caucasian, Aflican black and Japanese have strong tendency to form unique groups composed of mostly the same spieces. In this study, we found that Chinese alleles often had the same motif as that of Japanese alleles resulting in the groups consist of only Japanese and Chinese alleles. Thus, using MVR-PCR allele codes and flanking haplotypes, we could show that Chinese alleles were chosely related to Japanese alleles. Allele mapping of D16S309 (MS205) was also performed with Japanese DNA specimens. We can usually obtain MVR-PCR maps of whole alleles at this locus because the length of the most alleles is short. We found that Japanese had rather short alleles, and their internal structures were somewhat diffrenent from those of Caucasians. We also showed the usufullness of MVR-PCR mapping for paternity testings using practical cases.

去年，我们获得了100个中国人的DNA样本，并研究了D1S8 （MS32）和D7S21 （MS31A）位点侧DNA的三个多态性位点的等位基因频率。将中国人的单倍型频率与已有报道的日本人和白种人的单倍型频率进行比较。中国人的单倍型分布与日本人无显著差异，但与高加索人有显著差异。利用等位基因特异性PCR引物，从基因组DNA中选择性扩增单个等位基因，进行等位基因特异性MVR-PCR。虽然MVR-PCR等位基因编码具有高度多态性，但内部结构的相似性可以通过点阵分析来分析。此外，不同的等位基因可能具有相关的侧翼单倍型，这可能是由于它们拥有最近的共同祖先。用点阵分析方法对MS32等位基因或MS31A等位基因进行比对，我们已经发现，白种人、非洲黑人和日本人有很强的倾向，形成由大部分相同的物种组成的独特群体。在本研究中，我们发现中国人的等位基因往往与日本人的等位基因具有相同的基序，导致群体中只有日本人和中国人的等位基因。因此，利用MVR-PCR等位基因编码和侧翼单倍型，我们可以证明中国等位基因与日本等位基因有选择性相关。D16S309 （MS205）的等位基因定位也与日本DNA标本进行了比较。由于大多数等位基因的长度较短，我们通常可以获得该位点的全等位基因的MVR-PCR图谱。我们发现日本人的等位基因较短，其内部结构与白种人有所不同。我们还展示了MVR-PCR图谱在亲子鉴定中的实用性。