权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Elucidation the mechanism of the immunological changes during anesthesia and abdominal surgery

阐明麻醉和腹部手术过程中免疫学变化的机制

基本信息

批准号：
09307038
负责人：
HOSOKAWA Toyoshi
金额：
$ 17.34万
依托单位：
Kyoto Prefectural University of Medicine
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (A)
财政年份：
1997
资助国家：
日本
起止时间：
1997 至 1998
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09307038/
关键词：
immune anesthesia surgical stress stress sympathetic nerve system サイトカイン Tリンパ球ヘルペ-Tリンパ球

项目摘要

One of the main purpose of this study was to develop the new method to analyze cytokine development pattern of helper T lymphocyte and natural killer cell. The challenging new method was following,1) Extract helper T lymphocyte and natural killer cell from subpopulations of mononuclear cell directly utilizing flow cytometry.2) Count the cell number of developing cytokine from subpopulations of mononuclear cell by ELIS POT method.3) Quantification of the differentiation to Th1 or Th2 and the change of the developmental ratio of the cytokines.The results were following,1 )We could not develop the methods to gain the complete reproducibility with flow cytometry utilizing double stain with LAG-3 and CD3O.2)The method to evaluate the change of cytokine development such as TNFgamma and lL-4 was not enough to catch the delicate change of helper T lymphocuytes.3)We are developing the new method to evaluate the change of helper T lymphocuytes utilizimg another markers such as TNFbeta, IL-5, IL-1O,IL-13 with cd4. The method has not be established yet but we will soon succeed in the develop of the method.4)To evaluate the change of the rates of gammadelta-T cell which is quite important as responding cell and participate to apotosis depended on the development of heat shock protein, we used the flow cytometry by the double stain with CD3 and TCR gamma/delta.5)The change of the rates of gammadelta-T cell in the peripheral blood during upper abdominal surgery was recognize.

本研究的主要目的之一是建立一种新的方法来分析辅助性T淋巴细胞和自然杀伤细胞的细胞因子发育模式。本研究采用流式细胞仪直接从单个核细胞亚群中提取辅助性T淋巴细胞和自然杀伤细胞（NK细胞），用酶联免疫斑点法（ELIS-POT）计数单个核细胞亚群中产生的细胞因子的数量，并定量分析其向Th 1或Th 2的分化及细胞因子发育率的变化。（1）目前尚不能建立一种完全重复性的LAG-3和CD_3 O双染流式细胞术检测方法;（2）目前对TNF-γ和IL-4等细胞因子的检测方法尚不能准确反映辅助性T淋巴细胞的变化;我们正在开发一种新的方法来评估辅助性T淋巴细胞的变化，利用另一种标志物，如TNF β，IL-5，IL-10，IL-13与cd 4。该方法尚未建立，但我们将很快成功地开发的方法。4）评估的变化，γ δ-T细胞，这是非常重要的反应细胞和参与凋亡依赖于热休克蛋白的发展，采用流式细胞术，用CD 3和TCR γ/δ双重染色。对上腹部手术患者外周血T细胞进行了检测。