Cardiac electrophysiology evaluation system for improved drug assessment

用于改进药物评估的心脏电生理学评估系统

• 批准号: 22K12801
• 负责人: 李 俊君
• 金额: $ 2.66万
• 依托单位: Osaka University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2022
• 资助国家: 日本
• 起止时间: 2022-04-01 至 2025-03-31
• 项目状态: 未结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22K12801/
• 关键词: Pluripotent stem cells; Cardiomyocytes; Maturation; Drug assessment; drug assessment; cardiomyocytes; maturation; pluripotent stem cells

We planned to reproduce the traveling wave on 2D plate and optimize the best condition for obtain matured hiPSC derived cardiomyocytes, to compare the traveling wave matured cardiomyocytes with adult cells. The achievements in the year include: 1. The traveling wave has been generated in cardiac tissue cultured on both plate and nanofiber sheets. The electrical signal of cardiac tissue could be recorded by using MEA. 2.The maturity of traveling wave matured cardiomyocytes has been confirmed by using staining, TEM, RNAsequencing and other methods. Their maturation level has been compared with those from human adult. 3. We published 2 papers and gave an oral presentation in 87th Annual Scientific Meeting of the Japanese Circulation Society, and obtained the young investigator award.

我们计划在二维平板上重现行波，并优化获得成熟hiPSC衍生心肌细胞的最佳条件，以比较行波成熟心肌细胞与成体细胞。年内取得的成绩包括：1.在平板和平板上培养的心脏组织中产生了行波。利用微电极可记录心脏组织的电信号。2.通过染色、透射电镜、RNA测序等方法证实了行波诱导的心肌细胞成熟。并将其成熟水平与成人进行了比较。3.在日本循环学会第87届学术年会上发表论文2篇，并作口头报告，获得青年研究者奖。

项目成果

Human iPSC-derived closed-loop cardiac tissue for improved drug assessment

人类 iPSC 来源的闭环心脏组织可改善药物评估

• 发表时间: 2023
• 作者: Ying Hua;Junjun Li;Xiang Qu;Jingbo Zhang;Masako Ishida;Noriko Yoshida;Hayato Miyoshi;Mikio Shiba3;Shuichiro Higo4,5;Nagako Sougawa1;Maki Takeda1;Takuji Kawamura1;Ryohei Matsuura;Toshihiko Toyofuku;Yoshiki Sawa;Li Liu and Shigeru Miyagawa
• 通讯作者: Li Liu and Shigeru Miyagawa