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Development of a novel co-culture model of NH4-tolerant propionate-oxidizing bacteria and methane-producing archaea for the recovery of CH4 under high NH4+

Development of a novel co-culture model of NH4-tolerant propionate-oxidizing bacteria and methane-producing archaea for the recovery of CH4 under high NH4+
开发耐 NH4 丙酸氧化细菌和产甲烷古菌的新型共培养模型,用于在高 NH4 条件下回收 CH4
批准号:
22K12428
负责人:
Luong Van・Duc
金额:
$2.66万
依托单位:
依托单位国家:
日本
项目类别:
Grant-in-Aid for Scientific Research (C)
财政年份:
2022
资助国家:
日本
项目状态:
未结题
起止时间:
2022-04-01 至 2025-03-31

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中文摘要
翻译
我们通过批量转移的方法成功地浓缩了耐氨丙酸氧化菌。富集菌在5gNH4-N/L条件下能氧化丙酸。下一代测序表明,丙酸氧化菌的优势菌包括尖顶杆菌和隐气杆菌。此外,还观察到了产氢甲烷菌数量的增加,表明耐氨丙酸氧化细菌与产氢产甲烷菌之间存在着合养关系。
英文摘要
We have successfully enriched NH4-tolerant propionate-oxidizing bacteria by batch transfers. The enrichment culture can oxidize propionate under 5 gNH4-N/L. Next-generation sequencing showed the dominance of propionate-oxidizing bacteria, including Pelotomaculum and Cryptanaerobacter. In addition, the increase of hydrogenotrophic methanogens Methanoculleus was also observed, indicating the syntrophic relationship between NH4-tolerant propionate-oxidizing bacteria and hydrogenotrophic methanogens.
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