Development of a novel co-culture model of NH4-tolerant propionate-oxidizing bacteria and methane-producing archaea for the recovery of CH4 under high NH4+

开发耐 NH4 丙酸氧化细菌和产甲烷古菌的新型共培养模型，用于在高 NH4 条件下回收 CH4

• 批准号: 22K12428
• 负责人: Luong Van・Duc
• 金额: $ 2.66万
• 依托单位: Osaka University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2022
• 资助国家: 日本
• 起止时间: 2022-04-01 至 2025-03-31
• 项目状态: 未结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22K12428/
• 关键词: anaerobic digestion; high ammonium; propionate; propionate oxidizers; carbon recovery; methane recovery; propionate oxidizer; methane producer

We have successfully enriched NH4-tolerant propionate-oxidizing bacteria by batch transfers. The enrichment culture can oxidize propionate under 5 gNH4-N/L. Next-generation sequencing showed the dominance of propionate-oxidizing bacteria, including Pelotomaculum and Cryptanaerobacter. In addition, the increase of hydrogenotrophic methanogens Methanoculleus was also observed, indicating the syntrophic relationship between NH4-tolerant propionate-oxidizing bacteria and hydrogenotrophic methanogens.

我们已经成功地丰富了NH 4耐受丙酸氧化细菌的批量转移。富集培养物在5gNH_4-N/L以下能氧化丙酸盐。下一代测序显示丙酸氧化细菌占优势，包括Pelotomaculum和Cryptanaerodium。此外，还观察到氢营养型产甲烷菌Methanoculleus的增加，表明耐NH 4丙酸盐氧化细菌和氢营养型产甲烷菌之间存在互养关系。