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Studies on origin of retroviruses from wild carnivore

野生食肉动物逆转录病毒起源的研究

基本信息

批准号：
09041150
负责人：
MIYAZAWA Takayuki
金额：
$ 6.14万
依托单位：
The University of Tokyo
依托单位国家：
日本
项目类别：
Grant-in-Aid for international Scientific Research
财政年份：
1997
资助国家：
日本
起止时间：
1997 至 1998
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09041150/
关键词：
Vietnam feline immunodeficiency virus feline herpesvirus type 1 feline leukemia virus feline svncvtial virus feline calicivirus feline parvovirus leopard cat

项目摘要

In Socialist Republic of Vietnam (Vietnam), there are lots of camivors (feuds) which are not present in Japan. Three retroviruses (feline leukemia virus (FIV), feline immunodeficiency virus (FIV), and feline syncytial virus (FSV)) are known to infect domestic cats. The purpose of this study is to isolate and identify retroviruses from several feuds in Vietnam and to compare them with known retroviruses. We surveyed domestic and leopard cats in the vicinity of Hanoi and HoChiMinh city.In the vicinity of Hanoi, we could not find any incidence of infection of FIV or FeLV in both domestic and leopard cats. However, we found that 29% of domestic cats and 71% of leopard cats were infected with FSV.We isolated 9 strains of FSV and conducted phylogenetic analysis. All the isolates except one isolate formed a cluster in the phylogenetic tree. Form the result, we considered that FSV has been present in Vietnam from the earliest time or FIV of domestic cats infect leopard cats recently, or vice versa.In the vicinity of HoChiMinh city, we could not find any incidence of infection of FeLV in both domestic and leopard cats. Contrary to the result obtained in the vicinity of Hanoi city, we found that 22% of domestic cats were ***ected with FIV whereas no leopard cats were infected with the virus. In addition, we found that 78% of domestic cats and 25% of leopard cats were infected with FSV.We succeeded in isolation of five strains of FIV and determined the nucleotide sequence of the V3-V5 legion of env gene. The phylogenetic analysis revealed that five strains belonged to subtype C and one strain belonged to subtype D.FIV Subtype C is reported to be prevalent in Canada and Taiwan. From the results obtained here, we considered that FIV invaded southern Vietnam recently from other countries including Canada and Taiwan, or domestic cats in southern Vietnam retained FIV subtype C from the earliest time.

在越南社会主义共和国(越南)，有许多日本没有的海盗(世仇)。已知有三种逆转录病毒(猫白血病病毒(FIV)、猫免疫缺陷病毒(FIV)和猫合胞病毒(FSV))感染家猫。本研究的目的是从越南的几个世仇中分离和鉴定逆转录病毒，并将它们与已知的逆转录病毒进行比较。我们对河内和胡志明市附近的家猫和豹猫进行了调查，在河内附近没有发现家猫和豹猫感染FIV或FeLV的病例。然而，我们发现29%的家猫和71%的豹猫感染了口蹄疫。我们分离到9株口蹄疫病毒并进行了系统发育分析。除1个菌株外，其余菌株在系统发育树上均聚为一簇。由此可见，越南早在家猫感染豹猫时就存在口蹄疫，反之亦然。在胡志明市附近地区，未发现家猫和豹猫感染口蹄疫病毒的病例。与河内附近地区的结果相反，我们发现22%的家猫感染了FIV，而没有豹猫感染病毒。此外，我们还发现78%的家猫和25%的豹猫感染了FIV。我们成功地分离出了5株FIV，并测定了env基因V3-V5军团的核苷酸序列。系统进化分析表明，5株毒株属于C亚型，1株属于D亚型。据报道，C亚型FIV在加拿大和台湾地区流行。从这些结果中，我们认为FIV是最近从加拿大和台湾等其他国家入侵越南南部的，或者是越南南部的家猫最早保留了FIV C亚型。