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Studies on origin of retroviruses from wild carnivore

野生食肉动物逆转录病毒起源的研究

基本信息

批准号：
09041150
负责人：
MIYAZAWA Takayuki
金额：
$ 6.14万
依托单位：
The University of Tokyo
依托单位国家：
日本
项目类别：
Grant-in-Aid for international Scientific Research
财政年份：
1997
资助国家：
日本
起止时间：
1997 至 1998
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09041150/
关键词：
Vietnam feline immunodeficiency virus feline herpesvirus type 1 feline leukemia virus feline svncvtial virus feline calicivirus feline parvovirus leopard cat

项目摘要

In Socialist Republic of Vietnam (Vietnam), there are lots of camivors (feuds) which are not present in Japan. Three retroviruses (feline leukemia virus (FIV), feline immunodeficiency virus (FIV), and feline syncytial virus (FSV)) are known to infect domestic cats. The purpose of this study is to isolate and identify retroviruses from several feuds in Vietnam and to compare them with known retroviruses. We surveyed domestic and leopard cats in the vicinity of Hanoi and HoChiMinh city.In the vicinity of Hanoi, we could not find any incidence of infection of FIV or FeLV in both domestic and leopard cats. However, we found that 29% of domestic cats and 71% of leopard cats were infected with FSV.We isolated 9 strains of FSV and conducted phylogenetic analysis. All the isolates except one isolate formed a cluster in the phylogenetic tree. Form the result, we considered that FSV has been present in Vietnam from the earliest time or FIV of domestic cats infect leopard cats recently, or vice versa.In the vicinity of HoChiMinh city, we could not find any incidence of infection of FeLV in both domestic and leopard cats. Contrary to the result obtained in the vicinity of Hanoi city, we found that 22% of domestic cats were ***ected with FIV whereas no leopard cats were infected with the virus. In addition, we found that 78% of domestic cats and 25% of leopard cats were infected with FSV.We succeeded in isolation of five strains of FIV and determined the nucleotide sequence of the V3-V5 legion of env gene. The phylogenetic analysis revealed that five strains belonged to subtype C and one strain belonged to subtype D.FIV Subtype C is reported to be prevalent in Canada and Taiwan. From the results obtained here, we considered that FIV invaded southern Vietnam recently from other countries including Canada and Taiwan, or domestic cats in southern Vietnam retained FIV subtype C from the earliest time.

在越南社会主义共和国（越南），有很多camivors（世仇），这是不存在于日本。已知有三种逆转录病毒（猫白血病病毒（FIV）、猫免疫缺陷病毒（FIV）和猫合胞病毒（FSV））感染家猫。本研究的目的是分离和鉴定逆转录病毒在越南的几个封建，并与已知的逆转录病毒进行比较。我们调查了河内和胡志明市附近的家猫和豹猫。在河内附近，我们在家猫和豹猫中均未发现FIV或FeLV的感染。然而，我们发现29%的家猫和71%的豹猫感染了FSV。我们分离了9株FSV并进行了系统发育分析。在系统发育树中，除一个菌株外，其余菌株聚在一起。从结果来看，我们认为FSV在越南从最早的时候就已经存在，或者家猫的FIV最近感染了豹猫，反之亦然。在胡志明市附近，我们没有发现家猫和豹猫中FeLV的感染率。与在河内市附近获得的结果相反，我们发现22%的家猫感染了FIV，而没有豹猫感染该病毒。另外，家猫和豹猫的感染率分别为78%和25%。成功分离到5株FIV，并测定了其env基因V3-V5区的核苷酸序列。系统发育分析显示，5株属于C亚型，1株属于D亚型。据报道，FIV C亚型在加拿大和台湾流行。从这里获得的结果，我们认为，FIV入侵越南南部最近从其他国家，包括加拿大和台湾，或家猫在越南南部保留FIV亚型C从最早的时间。