Genetic ecology of pathogenic fungi in Brazil

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In the present work, following new evidences were obtained.1. Genetic identification method of Trichophyton tonsurans, a dermatophyte which is highly distributed in world wide as European and both American countries but low in Asia, was established. Furthermore, it was demonstrated that there is no genetically different among the strains isolated from various regions of the world.2. To study genetic variations of three dematiaceous fungi, Cladophialophora carrioni, Fonsecaea pedrosoi and Phialophora verrucosa which are causative agents of chromoblastomycosis, random amplified polymorphic DNA analysis (RAPD) was applied to the isolates collected from vaiious areas of the world. The results showed that the strains isolated from the same country or area had genetically high homogeneous pattern differed from those of other areas.3. The physiological and morphological features of Paracoccidioides brasiliensis, the causative agent of paracoccidioidomycosis which is a deep mycosis limited to Latin America, were studied in order to define their phenotype. RAPD analysis for the strains collected in Brazil showed that the tested strains could be separated in two groups genetically.4. The newly designated primers from known cytochrome b amino acid sequences were found to be useful for identification and phylogenetic study of pathogenic species of Aspergillus.