Genetic ecology of pathogenic fungi in Brazil

巴西病原真菌的遗传生态学

• 批准号: 09041173
• 负责人: FUKUSHIMA Kazutaka
• 金额: $ 4.16万
• 依托单位: Chiba University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for international Scientific Research
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 1998
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09041173/
• 关键词: pathogenic fungi; eology; molecular epidemiology; Brazil; gene; dematiaceous fungi; dermatophytes

In the present work, following new evidences were obtained.1. Genetic identification method of Trichophyton tonsurans, a dermatophyte which is highly distributed in world wide as European and both American countries but low in Asia, was established. Furthermore, it was demonstrated that there is no genetically different among the strains isolated from various regions of the world.2. To study genetic variations of three dematiaceous fungi, Cladophialophora carrioni, Fonsecaea pedrosoi and Phialophora verrucosa which are causative agents of chromoblastomycosis, random amplified polymorphic DNA analysis (RAPD) was applied to the isolates collected from vaiious areas of the world. The results showed that the strains isolated from the same country or area had genetically high homogeneous pattern differed from those of other areas.3. The physiological and morphological features of Paracoccidioides brasiliensis, the causative agent of paracoccidioidomycosis which is a deep mycosis limited to Latin America, were studied in order to define their phenotype. RAPD analysis for the strains collected in Brazil showed that the tested strains could be separated in two groups genetically.4. The newly designated primers from known cytochrome b amino acid sequences were found to be useful for identification and phylogenetic study of pathogenic species of Aspergillus.

本工作取得了以下新证据： 1．建立了断发毛癣菌的遗传鉴定方法，该皮肤癣菌在世界范围内以欧美国家分布较多，而在亚洲分布较少。进一步证明，世界各地分离的菌株之间不存在遗传差异。 2．为了研究三种深色真菌（Cladophialophora carrioni、Fonsecaea pedrosoi 和 Phialophora verrucosa）的遗传变异（这三种颜色芽生菌病的病原体），对从世界各地收集的分离株进行了随机扩增多态性 DNA 分析 (RAPD)。结果表明，同一国家或地区分离的菌株具有与其他地区不同的遗传高度同质性模式。 3．对巴西副球孢子菌病（一种仅限于拉丁美洲的深部真菌病）的病原体巴西副球孢子菌病的生理和形态特征进行了研究，以确定其表型。对巴西采集的菌株进行RAPD分析表明，供试菌株在遗传上可分为两组。4．发现来自已知细胞色素b氨基酸序列的新指定引物可用于曲霉病原菌种的鉴定和系统发育研究。

项目成果

Francisco H.Aoki et al.: "New PCR primer pairs specific for Cryptococcus neoformans serotype A or B prepared on the basis of random amplified polymorphic DNA fingeerprint pattern analyses." J.Clin.Microbiol. (in press).

Francisco H.Aoki 等人：“基于随机扩增多态性 DNA 指纹模式分析，制备了针对新型隐球菌血清型 A 或 B 的新 PCR 引物对。”

Sano, A.et al.: "Characteristics of 17 Paracoccidioides brasiliensis" Mycoscience. 38. 117-122 (1997)

Sano, A.等人：“17 种巴西副球孢子菌的特征”Mycoscience。

Jin Cui: "Histopathological and electron microscopial studies on experimental Penicillium marneffei infecton in mice." J. Med. Vet. Mycol.35. 347-353 (1997)

Jin Cui：“实验性马尔尼菲青霉菌感染小鼠的组织病理学和电子显微镜研究。”

K.Okada et al.: "Extraction and characterization of proteins from cell walls of two strains of Paracoccidioides brasiliensis." Boletin Micologico.13. 117-121 (1998)

K.Okada 等人：“从两种巴西副球孢子菌菌株的细胞壁中提取和表征蛋白质。”

Franclsco H.Aoki et al: "New PCR primer palrs speclfic for Cryptococcus neoformans serotype A or B prepared on the basis of random amplified polymorphic DNA fingerprint nattern analyses" J.Clin.Bicrobiol.(in press).

Franclsco H.Aoki 等人：“根据随机扩增多态性 DNA 指纹图谱分析制备的新型隐球菌血清型 A 或 B 特异性的新 PCR 引物”J.Clin.Bicrobiol.（出版中）。