Synthesis of Novel Fluorescent Carbohydrates

以CMP-NANA为能量供体，以烷基氨基乳糖苷为能量受体，立体选择性地合成了CMP-NANA作为唾液酸转移酶的底物。当以280 nm激发时，这些底物显示出清晰的发射光谱，其中发射峰分别位于340和525 nm左右，这是由于2-萘基和丹磺酰基的发射。合成底物被用作基于荧光能量转移的SiaT的底物。Vmax和Km分别测定为0.119 mol/min和0.85 M。这些底物提供了巨大的优势，例如灵敏度、速度和连续测定的可行性。

A CMP-NANA containing fluorescence probe as an energy donor and an alkylactosaminide containing fluorescence probe as an energy acceptor was successfully synthesizedegio- and stereoselectively as substrates for sialyl transferase. These substrates showed clear emission spectrum in which emission peaks were at around 340 and 525 nm due to 2-naphthyl and dansyl emissions, respectively, when the substrates was excited at 280nm. The synthetic substrates were used as substrates for SiaT based on fluorescence energy transfer. Vmax and Km were determined as 0.119 mol/min and 0.85 M, respectively. These substrates provide tremendous advantages such as sensitivity, speed, and feasibility of continuous assay.