Research on Retinal Transplantation of Neural Progenitors

神经祖细胞视网膜移植研究

基本信息

  • 批准号:
    10044272
  • 负责人:
  • 金额:
    $ 5.12万
  • 依托单位:
  • 依托单位国家:
    日本
  • 项目类别:
    Grant-in-Aid for Scientific Research (A).
  • 财政年份:
    1998
  • 资助国家:
    日本
  • 起止时间:
    1998 至 1999
  • 项目状态:
    已结题

项目摘要

Mechanical injury was induced in the adult rat retina by a hooked needle. A cell suspension (containing 90,000 neural stem cells) was slowly injected into the vitreous space. The specimens were processed for immunohistochemical studies at one, two and four weeks after the transplantation.After the mechanical injury, incorporation of the grafted stem cells in the host retinas was found as early as one week after transplantation. lmmunohistochemical studies showed that, at one week, more than half of the grafted cells expressed nestin, a marker for neural precursor cells, but not other markers. At four weeks, some grafted cells showed immunoreactivity for microtubule-associated protein (MAP) 2ab, MAP5, and glial fibrillary acidic protein (GFAP), suggesting progress in differentiation into cells of neuronal and astroglial lineages. However, they showed no immunoreactivity for HPC-1, calbindin and rhodopsin, suggesting that they did not differentiate into mature retinal neurons. lmmunoelectron microscopy revealed the formation of synapse-like structures between graft and host cells.By the manipulation of mechanical injury, the incorporation and subsequent differentiation of the grafted stem cells into neuronal and glial lineage, including the formation of synapse-like structures, can be achieved even in the adult rat retina.
用钩针在成年大鼠视网膜中引起机械损伤。将细胞悬液(含有 90,000 个神经干细胞)缓慢注入玻璃体腔。移植后一、两周和四周对标本进行处理以进行免疫组织化学研究。机械损伤后,早在移植后一周就发现移植干细胞掺入宿主视网膜中。免疫组织化学研究表明,一周后,超过一半的移植细胞表达巢蛋白(一种神经前体细胞的标记物),但不表达其他标记物。第四周时,一些移植细胞表现出微管相关蛋白 (MAP) 2ab、MAP5 和胶质纤维酸性蛋白 (GFAP) 的免疫反应性,表明在分化为神经元和星形胶质细胞谱系的细胞方面取得了进展。然而,它们对 HPC-1、钙结合蛋白和视紫质没有表现出免疫反应性,这表明它们没有分化成成熟的视网膜神经元。免疫电子显微镜揭示了移植物和宿主细胞之间突触样结构的形成。通过机械损伤的操作,移植的干细胞掺入并随后分化为神经元和胶质细胞谱系,包括突触样结构的形成,甚至可以在成年大鼠视网膜中实现。

项目成果

期刊论文数量(0)
专著数量(0)
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会议论文数量(0)
专利数量(0)
Takahashi M 他: "REscue of Photoreceptor Degeneration in the RD Mouse by HIV Vector-mediated Gene Transfer." Invest.Ophthalmol.Vis.Sic.39,4. 1117-1117 (1998)
Takahashi M 等人:“通过 HIV 载体介导的基因转移挽救 RD 小鼠的光感受器变性。”Invest.Ophasemol.Vis.Sic.39,4 (1998)。
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Inatani M 他: "Identification and Distribution of Retinal Chadroitin Sulfate Proteoglycuns" Invest.Ophthalmol Vis Sci. 39,4. 1053-1053 (1998)
Inatani M 等人:“硫酸视网膜查德罗素蛋白多糖的鉴定和分布”Invest.Ophasemol Vis Sci 39,4(1998)。
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Inatani M.et al.: "Identification of a nervous tissue-specific chondroitin sulfate proteoglycan, neurocan, in developing rat retina"Invest Ophthalmol Vis Sci. 40・10. 2350-2359 (1999)
Inatani M.等人:“大鼠视网膜中神经组织特异性硫酸软骨素蛋白聚糖的鉴定”Invest Ophasemol Vis Sci 40・10(1999)。
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    0
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Honjo M.et al.: "Expression of ciliary neurotrophic factor activated by retinal Muller cells in eyes with NMDA-and kainic acid-induced neuronal death"Invest Ophthalmol Vis Sci. 41・2. 552-560 (2000)
Honjo M. 等人:“NMDA 和红藻氨酸诱导的神经元死亡中视网膜 Muller 细胞激活的睫状神经营养因子的表达”Invest Ophasemol Vis Sci 41・2(2000)。
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    0
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Nishida A 他: "Clonal Adult Hippocampus-derired Stem Cells Can Migrate Into Injured Adult Rat Retine" Invest.Ophthalmol.Vis.Sci.39,4. 19-19 (1998)
Nishida A 等人:“克隆成年海马干细胞可以迁移到受伤的成年大鼠视网膜中”Invest.Ophasemol.Vis.Sci.39,4 (1998)。
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TAKAHASHI Masayo其他文献

TAKAHASHI Masayo的其他文献

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{{ truncateString('TAKAHASHI Masayo', 18)}}的其他基金

Identification of neural stem cells in neural retina, ciliary body and iris
神经视网膜、睫状体和虹膜神经干细胞的鉴定
  • 批准号:
    13671834
  • 财政年份:
    2001
  • 资助金额:
    $ 5.12万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Differentiation and transplantation of pigmented epithelial cells from the eye with homebox gene transfer
通过同源盒基因转移从眼睛中分化和移植色素上皮细胞
  • 批准号:
    11671736
  • 财政年份:
    1999
  • 资助金额:
    $ 5.12万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Inhibition of proliferative vitreo retinopathy by controling the related transcription factors.
通过控制相关转录因子抑制增殖性玻璃体视网膜病变。
  • 批准号:
    09671797
  • 财政年份:
    1997
  • 资助金额:
    $ 5.12万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
The relationship between the RPE cell function and its expression of growth factor receptors.
RPE细胞功能与其生长因子受体表达之间的关系。
  • 批准号:
    04454441
  • 财政年份:
    1992
  • 资助金额:
    $ 5.12万
  • 项目类别:
    Grant-in-Aid for General Scientific Research (B)

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根据造血干细胞移植后甲基化年龄估算干细胞衰老现象
  • 批准号:
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Muse细胞移植治疗杜氏肌营养不良症的基础研究
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    23K05627
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    2023
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The pathophysiology and therapeutic approaches of graft-versus-host disease following allogeneic hematopoietic stem cell transplantation with a focus on the role of oral microbiota.
异基因造血干细胞移植后移植物抗宿主病的病理生理学和治疗方法,重点关注口腔微生物群的作用。
  • 批准号:
    23K15301
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Psychological and social support for patients and their family members after hematopoietic stem cell transplantation who visit multiple outpatient clinics
为造血干细胞移植后到多个门诊就诊的患者及其家属提供心理和社会支持
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干细胞移植后骨髓增生异常综合征线粒体遗传的预后意义
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Non-invasive assessment of 3D tissue constructs for regenerative medicine and cell transplantation
用于再生医学和细胞移植的 3D 组织结构的非侵入性评估
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NLRP6 regulates NK-DC interaction in stem cell transplantation
NLRP6 调节干细胞移植中 NK-DC 相互作用
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增强胰岛细胞移植活力的新治疗策略的评估
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    2895096
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建立利用胎盘细胞移植产生人-小鼠嵌合体的方法。
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