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Core D - Immune Monitoring Core

核心 D - 免疫监测核心

基本信息

批准号：
10153660
负责人：
MIRIAM MERAD
金额：
$ 30万
依托单位：
ICAHN SCHOOL OF MEDICINE AT MOUNT SINAI
依托单位国家：
美国
项目类别：
财政年份：
2020
资助国家：
美国
起止时间：
2020-06-01 至 2023-01-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10153660
关键词：
Address B-Lymphocytes Biological Assay CD8-Positive T-Lymphocytes Cell physiology Cells Cellular Assay Cellular Compartment Analysis Clinical Collaborations Cytometry Data Data Analyses Dendritic Cells Dengue Dengue Infection Dengue Vaccine Dengue Virus Development Disease Disease Outcome Enrollment Exposure to Fingerprint Future Gene Expression Profiling Generations Genes Genetic Genetic Transcription Genomics Goals Helper-Inducer T-Lymphocyte Human Human Characteristics Immune Immune response Immunity Immunocompetence Immunologic Monitoring Immunology Impairment In Vitro Individual Infection Innate Immune Response Longitudinal Studies Mass Spectrum Analysis Methods Modeling Myelogenous Myeloid Cells Natural Immunity Nicaragua Nicaraguan Office of Administrative Management Outcome Pathogenicity Patients Peptides Peripheral Blood Mononuclear Cell Phenotype Plasma Procedures Proteomics Sampling Serology Serotyping Standardization System Systems Analysis Systems Biology T cell response T-Lymphocyte Technology Testing Time Vaccinated Vaccination Vaccine Clinical Trial Vaccines Woman adaptive immune response adaptive immunity antiviral immunity base cohort cost data management immune function immunological status immunoregulation in vivo member men monocyte novel pathogenic virus response vaccination outcome

项目摘要

The Human Immune Monitoring Core will characterize phenotypic and functional, ex vivo and in vivo signature as well as analyses of innate and adaptive immune responses to DENV infection and challenges at various time points whereas information on humoral immune responses to DENV will be obtained from the longitudinal studies of infected patients described project 1. Together, these validated assays will generate data to be integrated with results from the genomic and proteomic cores, and contribute to a DENV signature with clinical value. We anticipate that advances in mass spectrometry technologies and sample miniturization procedures may increase the sensitivity of global and functional analyses and may reduce the amount of sample required for these analyses allowing to extend innate immune competence studies to infected and vaccinated patients further refining the identification of a signature correlated with clinical status upon DENV infection, vaccine outcome or DC pathogenicity. These assays comprise the groundwork for future development of new immune enhancing strategies to promote the development of a protective immune response.

人类免疫监测核心将表征离体和体内的表型和功能体内特征以及对DENV感染的先天性和适应性免疫应答的分析而关于体液免疫应答的信息， DENV将从项目1描述的感染患者的纵向研究中获得。这些经过验证的测定将共同生成数据，并与来自基因组和蛋白质组核心，并有助于具有临床价值的DENV签名。我们预计质谱技术和样品微型化的进步程序可能会增加全球和功能分析的敏感性，这些分析所需的样本量，允许扩展先天免疫能力对受感染和接种疫苗的患者进行的研究，与DENV感染后的临床状态、疫苗结果或DC致病性相关。这些测定法构成了未来开发新的免疫增强剂的基础。促进保护性免疫反应发展的策略。