Swine Core - Regional/National Shared Resources Core

摘要(猪心) 印第安纳州糖尿病研究中心的猪核心是一个地区/国家共享资源核心， 自2004年以来一直在运营和服务研究人员。猪芯的中心功能是减少 使用Ossabaw猪的障碍，这是一种非常接近人类新陈代谢的独特动物模型 综合征(METS)和进展为2型糖尿病，并伴随着长期的并发症。理解 治疗甲硫氨酸和糖尿病在人类中有许多长期的健康并发症 由于缺乏可翻译的动物模型而被扼杀。实验方法及其对人类临床的翻译 从广泛使用的啮齿动物和转基因小鼠模型中进行药物治疗是不可能的。猪芯将会 描述蛋氨酸与2型糖尿病和/或化学性糖尿病(Mets/D)的进展和 由此导致的Ossabaw猪的共病。我们已经向大约160头猪提供了Ossabaw组织和/或生猪 调查人员在猪芯行动的15年中。约50种不同的组织库，可从 每头猪都能广泛传播给其他调查人员，提供出色的资源共享 和成本效益。提供这种猪资源使我们能够测试关于 集成的体内发病机制和长期并发症，并为细胞和 分子机制。猪心是从更简单的动物转化为研究的关键界面 模型(胰岛和生理核心)到人类(翻译核心)。《猪芯》由M.Sturek博士执导， 他是猪的人类疾病建模领域的领导者，M.阿洛什博士是副主任。这个 猪核心的具体目标是： (1)。使瘦肉型和饮食诱导的蛋氨酸和糖尿病(Mets/D)猪随时可用，即“在货架上”。 (2)。测定蛋氨酸/D的内分泌、代谢和血脂紊乱指数以筛选最佳 表型。 (3)。提供用于分发的组织和数据库。 猪核心的这些目标将减少研究这种高度相关的动物模型的障碍，并将 促进将研究从更简单、更简单的模型逐步转化为人类。

ABSTRACT (SWINE CORE) The Swine Core of the Indiana Diabetes Research Center is a Regional/National Shared Resource Core that has been operating and servicing researchers since 2004. The central function of the Swine Core is to reduce barriers to the use of Ossabaw swine, a unique animal model that closely approximates human metabolic syndrome (MetS) and progression to type 2 diabetes, with attendant long-term complications. Understanding and therapeutic treatment of MetS and diabetes with numerous long-term health complications in humans has been stifled by the lack of translational animal models. Experimental methods and translation to human clinical medicine is not possible from widely used rodent and transgenic mouse models. The Swine Core will characterize MetS and progression to type 2 diabetes and/or chemically-induced diabetes (MetS/D) and the resulting comorbidities in Ossabaw swine. We have provided Ossabaw tissue and/or live pigs to ~160 investigators during the 15 years of the Swine Core operation. A bank of ~50 different tissues available from each pig has enabled widespread dissemination to other investigators, providing outstanding resource sharing and cost-effectiveness. Providing this swine resource enables testing of numerous hypotheses about the integrated, in vivo pathogenesis and long-term complications and provides tissues for studies of cellular and molecular mechanisms. The Swine Core is a critical interface in the translation of research from simpler animal models (Islet & Physiology Core) to humans (Translation Core). The Swine Core is Directed by Dr. M. Sturek, a leader in modeling human diseases in swine, and Dr. M. Alloosh serves as the Associate Director. The Specific Aims of the Swine Core are to: (1). Make lean and diet-induced MetS and diabetic (MetS/D) pigs readily available, i.e. “on the shelf”. (2). Determine the endocrine, metabolic, and dyslipidemia indices of MetS/D to screen for optimal phenotypes. (3). Provide a tissue and data bank for distribution. These aims of the Swine Core will reduce barriers to study of this highly relevant animal model, and will facilitate the progressive translation of research from simpler, less complex models to humans.