足细胞内质网应激与肾病综合征-猫眼课题宝

足细胞内质网应激与肾病综合征

批准号：

10161772

负责人：

Ying Maggie Chen

金额：

$30.5万

依托单位：

WASHINGTON UNIVERSITY

依托单位国家：

美国

项目类别：

财政年份：

2017

资助国家：

美国

项目状态：

已结题

起止时间：

2017-08-15 至 2024-05-31

来源：

https://reporter.nih.gov/project-details/10161772

关键词：

Ablation Accounting Apoptosis Apoptotic Astrocytes Attenuated Cell Surface Receptors Cessation of life Coupled DNA Sequence Alteration Development Diagnosis End stage renal failure Endoplasmic Reticulum Engineering Functional disorder Genes Genetic Genetic Models Genetic Variation Goals Human Human Genetics Induced Mutation Injury Kidney Kidney Diseases Kidney Failure Knock-out Knockout Mice Laminin Lead Life Link Mediating Missense Mutation Molecular Molecular Target Morbidity - disease rate Mutate Mutation Nephrotic Syndrome Pathogenesis Pathogenicity Pathway interactions Patients Proteins Proteinuria Proto-Oncogene Proteins c-akt Regulation Renal function Research Research Personnel Risk Risk Assessment Role Signal Transduction Signaling Protein Testing Therapeutic Time Transcriptional Regulation Transgenic Mice Translational Research Uncertainty Universities Variant Washington base cohort cost disease phenotype disorder risk effective therapy endoplasmic reticulum stress genetic approach genetic variant glomerular basement membrane high throughput screening in silico in vivo innovation knock-down lamin B2 mortality mouse genetics mouse model mutant neurotrophic factor next generation sequencing novel novel therapeutic intervention novel therapeutics overexpression podocyte response tool transcription factor CHOP treatment strategy variant of unknown significance

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Abstract Nephrotic syndrome (NS) is characterized by heavy proteinuria and increased risk of loss of kidney function. It causes serious morbidity and high mortality, accounting for 15% of prevalent end-stage renal disease at an annual cost of more than $3 billion in the US. There is no effective treatment for most cases of NS caused by genetic mutations. Our research has been focused on a monogenic form of NS caused by a missense mutation (C321R) of LAMB2, one of the most commonly mutated genes in NS. Laminin 2 encoded by LAMB2 is a component of laminin-521 ( 5 2 1), the major laminin trimer of the glomerular basement membrane (GBM). Using our established knockout/transgenic mouse model, we have found that podocyte endoplasmic reticulum (ER) stress and subsequent defective secretion of misfolded C321R-LAMB2 from podocytes to the GBM lead to proteinuria. Furthermore, we have recently discovered an ER soluble factor that promotes the survival of ER-stressed podocytes. The overall goals of this proposal are to delineate molecular mechanisms regulating important death and survival pathways activated by the C321R-LAMB2 mutation-induced podocyte ER stress, to determine the contribution of podocyte ER stress in NS patients carrying putative deleterious missense variants, and to investigate novel treatment strategies for genetic forms of NS. To accomplish our research goals, we will utilize mouse genetic models and access two major human NS cohorts, Nephrotic Syndrome Study Network (NEPTUNE) and the Washington University Kidney Translational Research Core (WU KTRC). We have assembled an interdisciplinary team including multiple co-investigators and collaborators with the required expertise in pioneering ER stress research, human and mouse genetics, next generation sequencing, and high throughput screening. The proposed study will help us classify NS patients based on underlying molecular mechanisms, stratify disease risk, and discover highly-targeted treatments for NS patients caused by podocyte ER dysfunction.

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