Cytek 3-laser Aurora

Cytek 3 激光 Aurora

• 批准号: 10170799
• 负责人: KATHLEEN M BRUNDAGE
• 金额: $ 25.9万
• 依托单位: WEST VIRGINIA UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-04-01 至 2022-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10170799
• 关键词: Area; Award; Biomedical Research; Cells; Centers of Research Excellence; Clinical Sciences; Communities; Core Facility; Data Analyses; Flow Cytometry; Fluorochrome; Funding; Grant; Institutes; Institution; Lasers; National Institute of General Medical Sciences; Population; Research; Research Personnel; Resources; S10 grant; Sampling; Services; Technology; Time; Translational Research; United States National Institutes of Health; Universities; Virginia; Wait Time; West Virginia; cost; operation; programs; research facility; sample collection

PROJECT SUMMARY/ABSTRACT The West Virginia University (WVU) Flow Cytometry & Single Cell Core Facility (FCSCCF) is a centralized institutional shared research resource facility that serves the research community at WVU and other regional institutions. The WVU FCSCCF operates in accordance with best practices in operations and management for institutional shared research facilities. Services are driven by the needs of the users’ NIH-funded projects in biomedical research in 4 primary areas:1) investigator-initiated grants (R01 and R21), 2) grants from the National Institute of General Medical Sciences (NIGMS) Center of Biomedical Research Excellence (CoBRE P20 GM121322 and P20 GM109098), 3) NIGMS supported Clinical and Translational Science Institute (CTSI, U54 GM104942), and 4) NIGMS supported Idea Network for Biomedical Research Excellence (INBRE P20 GM103434) programs. Currently, two flow cytometers are available to researchers using the WVU FCSCCF: 1) a 4-laser Becton Dickinson (BD) LSRFortessa analytic flow cytometer, purchased with an NIH S10 grant (awarded in 2013) and 2) a 4-laser BD FACSAria III cell sorter, purchased in 2017 with funds from the WV CTSI. High usage of the LSRFortessa has caused some researchers to analyze their samples on the more expensive and slower FACSAria III cell sorter, which increases the time and cost of their research. In 2019, billed usage for analytical samples was 85.6% of the accessible users time (AUT) (1189.4 hr (71.5% AUT) on the LSRFortessa and 235.8 hr (14.2% AUT) on the FACS Aria III). In the past year, two users have needed to detect more than the maximum of 17 fluorochromes that the LSRFortessa can detect in a single sample and have been sending samples to the University of Virginia’s flow cytometry core for analysis on a Cytek Aurora. By sending samples to another institution, researchers incur a higher cost and longer lag times between sample collection and data analysis. Additionally, several WVU FCSCCF users study rare cell populations, which is particularly challenging when users are forced to analyze samples on the FACSAria III. After evaluating several analytical flow cytometers, we determined that the Cytek 3-laser Aurora has the best combination of features to meet the WVU FCSCCF users’ current and anticipated needs. Addition of the Cytek flow cytometer will support our NIH-funded investigators by increasing access to cutting edge technology and reducing wait time and costs, thereby increasing competitiveness.

项目总结/摘要 西弗吉尼亚大学（WVU）流式细胞术和单细胞核心设施（FCSCCF）是一个集中的 机构共享的研究资源设施，为研究界在西弗吉尼亚大学和其他区域 机构职能体系WVU FCSCCF按照运营和管理方面的最佳实践运作 用于机构共享研究设施。服务由用户的NIH资助项目的需求驱动 生物医学研究在4个主要领域：1）发起人发起的赠款（R 01和R21），2）赠款从 国家普通医学科学研究所（NIGMS）生物医学研究卓越中心（CoBRE P20 GM 121322和P20 GM 109098），3）NIGMS支持的临床和转化科学研究所（CTSI， U 54 GM 104942），以及4）NIGMS支持的生物医学研究卓越理念网络（INBRE P20 GM 103434）程序。目前，两个流式细胞仪可供研究人员使用WVU FCSCCF： 1)4激光Becton Dickinson（BD）LSRFortessa分析流式细胞仪，由NIH S10资助购买 （2013年授予）和2）4激光BD FACSAria III细胞分选机，于2017年由WV资助购买 CTSI。LSRFortessa的高使用率导致一些研究人员在更多的环境中分析他们的样本。 昂贵且较慢的FACSAria III细胞分选仪，这增加了他们研究的时间和成本。在2019年， 分析样本的计费使用是可访问用户时间（AUT）的85.6%（1189.4小时（71.5% AUT）） 在LSRFortessa上为235.8小时（14.2%AUT），在FACS Aria III上为235.8小时（14.2%AUT）。在过去的一年里，两名用户 LSRFortessa单次检测最多可检测17种荧光染料， 样本，并已发送样本到弗吉尼亚大学的流式细胞术的核心进行分析， Cytek Aurora。通过将样本发送到另一个机构，研究人员承担了更高的成本和更长的滞后时间 在样本收集和数据分析之间。此外，几个WVU FCSCCF用户研究稀有细胞 当用户被迫在FACSAria III上分析样本时，这尤其具有挑战性。 在评估了几种分析型流式细胞仪后，我们确定Cytek 3-laser Aurora具有最好的 功能的组合，以满足WVU FCSCCF用户的当前和预期的需求。添加 Cytek流式细胞仪将通过增加尖端技术的使用来支持我们NIH资助的研究人员 技术，减少等待时间和成本，从而提高竞争力。