Evaluation of zoonotic reservoir hosts for severe fever with thrombocytopenia syndrome virus

项目摘要 严重发热伴血小板减少综合征病毒(SFTSV)是一种新出现的由硬蜱传播的人类病原体 中国、日本和韩国。最近在美国发现了这种名为长角血蜱的媒介 因此，国家可以在北美出现病毒的过程中发挥作用。地方性疾病监测工作 在家畜和所选野生动物中，区域显示血清转换和偶尔的病毒RNA，因此表明 人畜共患传播病毒的可能性。目前，我们对主持人能力和 在动物中的临床疾病是有限的，本研究试图阐明病毒扩增和 脊椎动物寄主的发病机制。这些信息将是了解SFTSV生态的关键，并将 为监督和监测工作提供指导。我们将为北美野生动物物种接种 与亚洲物种的生态相关性以寻找合适的水库宿主如果SFTSV出现在 美国。此外，我们将开发一个相关的载体-宿主模型来执行载体能力 用家养山羊对长角毛虫进行的研究，这可能是病毒的一个来源。这些实验是 预计将提供关于疾病传播和传播中感兴趣的物种的强有力的证据 提供有关刻度向量的重要信息。

Project Summary Severe fever with thrombocytopenia syndrome virus (SFTSV) is an emerging tick-borne human pathogen in China, Japan and South Korea. The vector, Haemaphysalis longicornis, was recently discovered in the United States, and as such, could play a role in viral emergence in North America. Surveillance efforts in endemic regions show seroconversion and occasional viral RNA in domestic animals and select wildlife, thus indicating the likelihood for zoonotic transmission of the virus. Presently, our knowledge about host competence and clinical disease in animals is limited, and this study seeks to elucidate the mechanism for viral amplification and pathogenesis in vertebrate hosts. This information will be key to understanding the ecology of SFTSV and will provide guidance for surveillance and monitoring efforts. We will inoculate North American wildlife species with ecological relevance to Asian species to look for competent reservoir hosts should SFTSV emerge in the United States. Additionally, we will develop a relevant vector-host model for performing vector competence studies with H. longicornis using domestic goats, which are a likely source of the virus. These experiments are expected to provide strong evidence regarding species of interest in disease transmission and spread and provide vital information about the tick vector.