Qualitative and Quantitative Determination of Pesticides in Beverages and Similar Food Matrices by High Performance Liquid Chromatography-Quadrupole Time-of-Flight (HPLC-QToF) Analysis

申请名称：新泽西食品测试计划-食品安全和国防（2020-2025） 项目名称：水果和蔬菜基杀虫剂的定性和定量测定 高效液相色谱-四极杆飞行时间（HPLC-QToF）分析 项目总结-学科D：特殊项目，分析轨道3：方法开发和验证 新泽西卫生部（NJDOH）正在寻求美国食品和药物管理局（FDA）的 实验室灵活供资模式合作协议，重点是提高实验室的能力 新泽西州维护食品安全和保障。化学恐怖主义、生物监测、药用植物和食品 测试实验室（CT实验室）设在NJDOH的环境化学实验室服务 （ECLS）将参与果汁和蔬菜的扩大农药方法的方法开发， 基于饮料。食品应急响应网络（FERN）方法CHE.0006和CHE.0008是 非常适合筛选食物中的毒素和毒物，包括一些杀虫剂，杀虫剂和除草剂。 然而，这些是范围广泛的方法，不包括商业应用的农药的完整列表， 因为这些主要是筛选方法，所以它们以大约百万分之一（ppm）水平的分析物为目标。低- 远低于百万分之一水平的杀虫剂可能无法检测到，并留在市场上的食品中。低级 如果食用农药，尤其是对婴儿或儿童，会造成更大的健康风险。 为了解决这一食品安全问题，一种能够分析超过65种农药的高灵敏度农药方法 将由CT实验室开发。实验室将利用其专业知识和经验， 大麻测试的方法，以创建一种可用于食品的方法，从果汁和蔬菜开始 饮料.这种新方法将目标分析物的数量扩大到超过68种农药，包括 2015年进行的新泽西州农药使用调查中列出的大多数农药， 限制降至十亿分之一（ppb）水平。这一能力将为FDA提供更多信息， 检测出杀虫剂的含量危及公众健康 CT实验室将使用这种方法来支持其他食品杀虫剂的研究， 根据FDA的要求，在各种食品中使用杀虫剂。通过开展本报告中详细介绍的工作， 建议和管理一个综合的食品安全系统，ECLS将能够扩大其食品防御， 安全活动，同时进一步保障公众健康，并为FDA提供额外的网络能力。

Application Title: New Jersey Food Testing Program - Food Safety and Defense (2020-2025) Project Title: Qualitative and Quantitative Determination of Pesticides in Fruit- and Vegetable-based Beverages by High Performance Liquid Chromatography-Quadrupole Time-of-Flight (HPLC-QToF) Analysis Project Summary – Discipline D: Special Projects, Analytical Track 3: Method Development and Validation The New Jersey Department of Health (NJDOH) is pursuing the U.S. Food and Drug Administration’s (FDA) Laboratory Flexible Funding Model cooperative agreement focused on improving the capability and capacity of NJ to maintain food safety and security. Chemical Terrorism, Biomonitoring, Medicinal Marijuana, and Food Testing Laboratory (CT Laboratory) housed within NJDOH’s Environmental Chemistry Laboratory Services (ECLS) will participate in the method development of an expanded pesticides method for juice- and vegetable- based beverages. The Food Emergency Response Network (FERN) methods CHE.0006 and CHE.0008 are excellent for the screening of food for toxins and poisons, including some pesticides, insecticides, and herbicides. However, these are wide ranging methods that do not include the full list of pesticides applied commercially and because the are primarily screening methods, they target analytes at around parts-per-million (ppm) levels. Low- level pesticides far below ppm levels can go undetected and remain in food items in markets. Low-level pesticides pose greater health risks if consumed, especially for babies or children. To address this food safety issue, a highly sensitive pesticide method capable of analyzing over 65 pesticides will be developed by the CT Laboratory. The laboratory will use its expertise and experience developing such a method for marijuana testing to create a method usable for food stuffs, starting with juice- and vegetable-based beverages. This new method will expand the number of target analytes to more than 68 pesticides, including most of pesticides listed in the NJ Pesticide Use Survey conducted in 2015, and will significantly lower detection limits down to parts-per-billion (ppb) level. This capability will provide more information for the FDA to determine measured pesticide levels that put the public’s health at risk. The CT Laboratory will use this method to support other food pesticide research by identifying and confirming the presence of pesticides in various foods as requested by the FDA. By conducting the work detailed in this proposal and managing an integrated food safety system, ECLS will be able to expand its food defense and safety activities while further safeguarding public health and provide the FDA with additional network capability.