Orbitrap Exploris 480 Basic System

项目摘要 我们提出了收购Thermo Scientific™ Orbitrap™ Exploris 480质量 光谱仪将安装在阿尔伯特·爱因斯坦医学院的蛋白质组学核心。这 仪器对于我们NIH资助的研究人员进行蛋白质组学实验至关重要。具体 本提案中提出的将从这一文书中受益匪浅的项目包括研究 传染病（单纯疱疹病毒、SARS-CoV 2、弓形虫、微孢子虫等），癌 （骨髓增生异常综合征，癌前和癌症干细胞的分子调控和靶向， 造血和白血病发生）、衰老（衰老期间的自噬、神经变性）、心血管 疾病和基础细胞生物学（肥胖，细胞死亡机制，染色质，细胞信号）。的 Einstein Proteomics Core需要此仪器来取代Thermo Scientific™ Orbitrap™ Velos。的 Orbitrap Velos于2010年被收购，它是Core唯一一个适合蛋白质组学的仪器，但它 现在需要更换，因为到2020年底，供应商将不再提供服务。实验室 蛋白质组学设施的空间最近已经完全翻新，Simone Sidoli博士被聘请 在爱因斯坦领导蛋白质组学研究在过去的一年里，蛋白质组学实验室运行了2,500多个样本， 大约50个不同的实验室爱因斯坦蛋白质组学核心也是一个关键的支持 服务于爱因斯坦癌症中心，内森休克中心卓越的生物学老化研究 （E-NSC）和ERC-CFAR Einstein-Rockefeller-CUNY艾滋病研究中心。

Project Summary We present our proposal for the acquisition of a Thermo Scientific™ Orbitrap™ Exploris 480 mass spectrometer that would be installed in the Proteomics Core at the Albert Einstein College of Medicine. This instrument is critical for the performance of proteomics experiments by our NIH funded investigators. Specific projects presented in this proposal that will benefit enormously from this instrument include research on infectious diseases (herpes simplex virus, SARS-CoV2, Toxoplasma gondii, microsporidia, etc.), cancer (myelodysplastic syndrome, molecular regulation and targeting of pre-cancerous and cancer stem cells in hematopoiesis and leukemogenesis), aging (autophagy during aging, neurodegeneration), cardiovascular diseases and fundamental cell biology (obesity, mechanisms of cell death, chromatin, cell signaling). The Einstein Proteomics Core requires this instrument to replace a Thermo Scientific™ Orbitrap™ Velos. The Orbitrap Velos was acquired in 2010, and it has been the only proteomics-fitted instrument for the Core, but it now needs to be replaced as by the end of 2020 it will no longer be serviced by the vendor. The laboratory space of the proteomics facility has recently been completely renovated and Dr. Simone Sidoli was hired to lead proteomics research at Einstein. In the last year, the proteomics laboratory ran over 2,500 samples from approximately 50 different investigators' laboratories. The Einstein Proteomics Core is also a critical support service for the Einstein Cancer Center, the Nathan Shock Center for excellence on Biology of Aging Research (E-NSC) and the ERC-CFAR Einstein-Rockefeller-CUNY Center for AIDS research.