Cancer Metabolism
Cancer Metabolism
批准号:
10174822
负责人:
Cosimo Commisso
金额:
$19.12万
依托单位国家:
美国
项目类别:
财政年份:
1997
资助国家:
美国
项目状态:
未结题
起止时间:
1997-05-01 至 2025-04-30
关键词:
Acyl Coenzyme AApplications GrantsBioenergeticsCancer CenterCancer Center Support GrantCell RespirationCellsCholesterolCitric Acid CycleCollaborationsCommunicationComputer softwareContract ServicesCore FacilityData AnalysesDetectionDoctor of PhilosophyEmployeeEquipmentExperimental DesignsFacultyFatty AcidsFeedbackFundingFutureGenus HippocampusGlucoseGlutamatesGlutamineGlycolysisGrantInstitutesLabelLeadershipMaintenanceMalignant NeoplasmsMass FragmentographyMeasuresMedicalMetabolicMetabolic PathwayMetabolismMethodologyMethodsMitochondriaNucleotidesOxidative PhosphorylationOxygen ConsumptionProductionPublicationsResearchResearch PersonnelResource SharingResourcesSamplingScienceScientistServicesSugar PhosphatesSurveysTechnologyTimeTumor-infiltrating immune cellsVolatile Fatty AcidsWorkanalytical methodanticancer researchcost effectivenessdesignexperienceextracellularinhibitor/antagonistinsightinstrumentinstrumentationinterestmeetingsmembermetabolic abnormality assessmentmetabolic phenotypemetabolomicsprogramsrepairedresponsestable isotopetemporal measurementtumor metabolism
中文摘要
摘要-癌症代谢共享资源
英文摘要
ABSTRACT – CANCER METABOLISM SHARED RESOURCE
The Cancer Metabolism Shared Resource provides comprehensive support for analysis of cancer-related
metabolism by investigators at the Sanford Burnham Prebys Medical Discovery Institute NCI-supported Cancer
Center. The services provided include assistance with the design of metabolic research programs and selection
of appropriate analytical methods, sample analysis using specialized equipment and methods, assistance with
data interpretation and publication (including response to reviewers), and assistance with grant proposals. Dr.
Scott, the facility Director, has extensive experience in metabolic analysis and serves as a resource for Center
members, educating scientists about the services offered, working to upgrade existing methods, and expanding
the range of analyses provided, both in response to requests from users and proactively in anticipation of future
demand. The Core provides three fundamental types of analysis. The first is quantitation of metabolites, either
through GC-MS analysis or a 96-well YSI analyzer. In the past funding period (the first for this Shared Resource),
a variety of enhancements have been made in quantification of metabolites such as fatty acids (including short-
chain fatty acids), cholesterol, sugars, and sugar phosphates, as well as more standard detection of glucose,
lactate, glutamine, and glutamate. The second approach provided in the Core is stable isotope tracing,
where(13C, 15N, 2H)-labeled substrates can be traced to metabolites such as TCA cycle intermediates and fatty
acids, which provides information on metabolic pathway flux that provides insight beyond metabolite
quantification alone. The third major analytical platform in the Core is a Seahorse XFp Extracellular Flux
Analyzer, purchased in 2015, which allows real-time measurement of cellular oxygen consumption and
extracellular acidification. Mitochondrial function and the relative contribution of glycolysis and oxidative
phosphorylation to ATP production can be measured in live by sequential addition of various mitochondrial
inhibitors or uncouplers. Over the last 5 years, the Cancer Metabolism Core has provided services to 24 Cancer
Center members, and supported at least 20 publications
期刊论文(0)
专著(0)
科研奖励(0)
会议论文
Discovery of Novel Inhibitors Targeting trans-Golgi Network Acidification in Pancreatic Cancer
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批准号:10563637
-
项目类别:
-
资助金额:$64.74万
-
财政年份:2023
-
负责人:Cosimo Commisso
-
依托单位:
Regulation and Function of Stromal Macropinocytosis in Pancreatic Ductal Adenocarcinoma
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批准号:10293358
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项目类别:
-
资助金额:$58.09万
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财政年份:2021
-
负责人:Cosimo Commisso
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依托单位:
Regulation and Function of Stromal Macropinocytosis in Pancreatic Ductal Adenocarcinoma
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批准号:10475282
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项目类别:
-
资助金额:$57.41万
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财政年份:2021
-
负责人:Cosimo Commisso
-
依托单位:
The Macropinosome: Uncovering the Molecular Anatomy of an Oncogene-driven Organelle
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批准号:10153719
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项目类别:
-
资助金额:$22.79万
-
财政年份:2020
-
负责人:Cosimo Commisso
-
依托单位:
Regulation of Nutrient Stress-Induced Macropinocytosis in Pancreatic Ductal Adenocarcinoma
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批准号:10475251
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项目类别:
-
资助金额:$45.68万
-
财政年份:2016
-
负责人:Cosimo Commisso
-
依托单位:
Regulation of Nutrient Stress-Induced Macropinocytosis in Pancreatic Ductal Adenocarcinoma
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批准号:10283951
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项目类别:
-
资助金额:$46.61万
-
财政年份:2016
-
负责人:Cosimo Commisso
-
依托单位:
Regulation of Nutrient Stress-Induced Macropinocytosis in Pancreatic Ductal Adenocarcinoma
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批准号:9280912
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项目类别:
-
资助金额:$44.61万
-
财政年份:2016
-
负责人:Cosimo Commisso
-
依托单位:
Regulation of Nutrient Stress-Induced Macropinocytosis in Pancreatic Ductal Adenocarcinoma
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批准号:10677661
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项目类别:
-
资助金额:$45.68万
-
财政年份:2016
-
负责人:Cosimo Commisso
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依托单位: