Cancer Metabolism

摘要-癌症代谢共享资源 癌症代谢共享资源为癌症相关分析提供全面支持 Sanford Burnham Prebys医学发现研究所NCI支持的癌症研究人员的新陈代谢 中心。所提供的服务包括帮助设计代谢研究方案和选择 适当的分析方法，使用专门的设备和方法进行样品分析，协助 数据解释和发布(包括对审查员的答复)，并协助拨款提案。Dr。 斯科特，设施主任，在代谢分析方面拥有丰富的经验，并担任中心的资源 成员，就所提供的服务教育科学家，努力升级现有方法，并扩展 提供一系列分析，既响应用户的请求，又主动预测未来 需求。核心提供了三种基本类型的分析。第一个是代谢物的定量。 通过GC-MS分析或96井YSI分析仪。在过去的筹资期间(该共享资源的第一个筹资期间)， 在对诸如脂肪酸(包括短链脂肪酸)等代谢物的定量方面进行了各种改进。 链脂肪酸)、胆固醇、糖和糖磷酸盐，以及更标准的葡萄糖检测， 乳酸、谷氨酰胺和谷氨酸。在核心中提供的第二种方法是稳定同位素示踪， 其中(13C，15N，2H)标记的底物可以追溯到代谢物，如TCA环中间体和脂肪 酸，它提供代谢途径流量的信息，提供对代谢物以外的洞察 单单是量化。核心中的第三个主要分析平台是海马XFP细胞外通量 Analyzer，于2015年购买，允许实时测量细胞氧耗和 胞外酸化。线粒体功能与糖酵解和氧化的相对贡献 通过顺序添加不同的线粒体，可以在活体中测量到对ATP产生的磷酸化 抑制剂或解偶联剂。在过去的5年里，癌症代谢核心已经为24个癌症患者提供了服务 中心成员，并支持至少20种出版物

ABSTRACT – CANCER METABOLISM SHARED RESOURCE The Cancer Metabolism Shared Resource provides comprehensive support for analysis of cancer-related metabolism by investigators at the Sanford Burnham Prebys Medical Discovery Institute NCI-supported Cancer Center. The services provided include assistance with the design of metabolic research programs and selection of appropriate analytical methods, sample analysis using specialized equipment and methods, assistance with data interpretation and publication (including response to reviewers), and assistance with grant proposals. Dr. Scott, the facility Director, has extensive experience in metabolic analysis and serves as a resource for Center members, educating scientists about the services offered, working to upgrade existing methods, and expanding the range of analyses provided, both in response to requests from users and proactively in anticipation of future demand. The Core provides three fundamental types of analysis. The first is quantitation of metabolites, either through GC-MS analysis or a 96-well YSI analyzer. In the past funding period (the first for this Shared Resource), a variety of enhancements have been made in quantification of metabolites such as fatty acids (including short- chain fatty acids), cholesterol, sugars, and sugar phosphates, as well as more standard detection of glucose, lactate, glutamine, and glutamate. The second approach provided in the Core is stable isotope tracing, where(13C, 15N, 2H)-labeled substrates can be traced to metabolites such as TCA cycle intermediates and fatty acids, which provides information on metabolic pathway flux that provides insight beyond metabolite quantification alone. The third major analytical platform in the Core is a Seahorse XFp Extracellular Flux Analyzer, purchased in 2015, which allows real-time measurement of cellular oxygen consumption and extracellular acidification. Mitochondrial function and the relative contribution of glycolysis and oxidative phosphorylation to ATP production can be measured in live by sequential addition of various mitochondrial inhibitors or uncouplers. Over the last 5 years, the Cancer Metabolism Core has provided services to 24 Cancer Center members, and supported at least 20 publications