Cancer Metabolism

摘要-癌症代谢共享资源 癌症代谢共享资源为癌症相关的分析提供全面的支持。 由Sanford Burnham Prebys医学发现研究所的研究人员进行的代谢研究 中心所提供的服务包括协助设计代谢研究计划和选择 适当的分析方法，使用专门的设备和方法进行样品分析， 数据解释和出版（包括对审查人员的答复），以及协助申请赠款。博士 Scott是设施总监，在代谢分析方面拥有丰富的经验，是中心的资源 成员，教育科学家所提供的服务，努力升级现有的方法，并扩大 所提供的分析范围，既响应用户的请求，也主动预测未来的 需求核心提供了三种基本类型的分析。首先是代谢物的定量， 通过GC-MS分析或96孔YSI分析仪。在过去的资助期间（本共享资源的第一个资助期间）， 在代谢物如脂肪酸（包括短链脂肪酸）的定量中已经进行了多种改进， 链脂肪酸）、胆固醇、糖和糖磷酸盐，以及更标准的葡萄糖检测， 乳酸盐谷氨酰胺和谷氨酸盐核心中提供的第二种方法是稳定同位素示踪， 其中（13 C，15 N，2 H）标记的底物可以追溯到代谢物，如TCA循环中间体和脂肪酸。 酸，它提供了代谢途径通量的信息，提供了超越代谢产物的见解 单独量化。核心中的第三个主要分析平台是Seahorse XFp细胞外通量 2015年购买的分析仪，可实时测量细胞耗氧量， 胞外酸化线粒体功能和糖酵解和氧化的相对贡献 磷酸化对ATP产生可通过依次加入各种线粒体 抑制剂或解偶联剂。在过去的5年里，癌症代谢中心为24名癌症患者提供了服务。 中心成员，并支持至少20个出版物

ABSTRACT – CANCER METABOLISM SHARED RESOURCE The Cancer Metabolism Shared Resource provides comprehensive support for analysis of cancer-related metabolism by investigators at the Sanford Burnham Prebys Medical Discovery Institute NCI-supported Cancer Center. The services provided include assistance with the design of metabolic research programs and selection of appropriate analytical methods, sample analysis using specialized equipment and methods, assistance with data interpretation and publication (including response to reviewers), and assistance with grant proposals. Dr. Scott, the facility Director, has extensive experience in metabolic analysis and serves as a resource for Center members, educating scientists about the services offered, working to upgrade existing methods, and expanding the range of analyses provided, both in response to requests from users and proactively in anticipation of future demand. The Core provides three fundamental types of analysis. The first is quantitation of metabolites, either through GC-MS analysis or a 96-well YSI analyzer. In the past funding period (the first for this Shared Resource), a variety of enhancements have been made in quantification of metabolites such as fatty acids (including short- chain fatty acids), cholesterol, sugars, and sugar phosphates, as well as more standard detection of glucose, lactate, glutamine, and glutamate. The second approach provided in the Core is stable isotope tracing, where(13C, 15N, 2H)-labeled substrates can be traced to metabolites such as TCA cycle intermediates and fatty acids, which provides information on metabolic pathway flux that provides insight beyond metabolite quantification alone. The third major analytical platform in the Core is a Seahorse XFp Extracellular Flux Analyzer, purchased in 2015, which allows real-time measurement of cellular oxygen consumption and extracellular acidification. Mitochondrial function and the relative contribution of glycolysis and oxidative phosphorylation to ATP production can be measured in live by sequential addition of various mitochondrial inhibitors or uncouplers. Over the last 5 years, the Cancer Metabolism Core has provided services to 24 Cancer Center members, and supported at least 20 publications