Replacement of an outdated 600 MHz NMR CryoProbe system

更换过时的 600 MHz NMR CryoProbe 系统

• 批准号: 10177236
• 负责人: Daoning Zhang
• 金额: $ 34.13万
• 依托单位: UNIV OF MARYLAND, COLLEGE PARK
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-04-15 至 2023-04-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10177236
• 关键词: Address; Affinity; Aging; Award; Biomolecular Nuclear Magnetic Resonance; Chemicals; Communities; Consumption; Ensure; Faculty; Funding; Generations; Government; Grant; Liquid substance; Maryland; Nitrogen; Nuclear Magnetic Resonance; Performance; Postdoctoral Fellow; Principal Investigator; Productivity; Research; Research Activity; Research Institute; Structure; System; Temperature; Time; United States National Institutes of Health; Universities; college; cost; experience; graduate student; hands-on learning; improved; instrument; instrumentation; interest; member; molecular dynamics; programs; repaired; tool

NIH Shared Instrumentation Grant (SIG) Program, PAR-20-113 Project Summary/Abstract: To sustain and expand the strong high field Biomolecular NMR research programs at the University of Maryland, College Park (UMCP), we are applying for a SIG grant to replace an aging Cryoprobe system of a Bruker 600 MHz Nuclear Magnetic Resonance (NMR) spectrometer at the Biomolecular NMR Facility. The components we propose to purchase are 1) a 5th generation CryoPlatform, 2) BNSL/a Nitrogen re-liquefication accessary, 3) a Cryo-TCI H&F probe, together with a BBFO room temperature probe, 4) a SampleCase system. The 600 MHz NMR equipped with a Cryo-TXI probe, installed in 2002, is the first high field NMR on UMCP campus. The spectrometer is a powerful tool to study chemical and biomolecular structures, molecular dynamics and affinities, as well as to analyze compounds in low concentration due to the Cryoprobe’s high sensitivity. Since its installation, the spectrometer has been daily serving graduate students and faculty members in various fields on campus, and periodically users from neighboring academic and government research institutes. It has been an essential instrument to several principal investigators (PIs) with NIH funded programs. However, the spectrometer has started to have increasing down time in recent years due to frequent repairs of its aging CryoPlatform and Cryo-TXI probe, disrupting NMR research activities of users. We believe it is urgent to address the reliability issue of the Cryoprobe system of the spectrometer to ensure the sustainability and performance of the system. The proposal for replacement is strongly supported by NIH funded PIs as well as other faculty members at UMCP. The SIG grant would provide an opportunity to rescue the aging instrument as well as to enhance the performance and capability of the instrument. If awarded, the up- to-date instrument will bring following benefits to UMCP NMR community: (1) Ensuring the advance of several NIH funded research programs. (2) Inspiring new Biomolecular NMR research interests. (3) Improving productivity and efficiency. (4) Providing hands-on learning and working experience to graduate students and postdocs (5) Drastically reducing liquid nitrogen consumption/cost.

NIH共享仪器赠款（SIG）计划，Par-20-113 项目摘要/摘要： 维持和扩展强大的高场生物分子NMR研究计划 马里兰州大学公园（UMCP），我们正在申请SIG赠款以替换 Bruker 600 MHz核磁共振（NMR）的老化的冷冻探针系统 生物分子NMR设施的光谱仪。我们建议购买的组件 是1）第五代冷冻平台，2）BNSL/a氮re-liquefication coscectary，3）a Cryo-TCI H＆F探针，以及BBFO室温探针，4）Samplecase 系统。 配备了Cryo-TXI探针的600 MHz NMR，2002年安装的是第一个高级 UMCP校园的NMR现场NMR。光谱仪是研究化学和 生物分子结构，分子动力学和亲和力以及分析 由于冷冻探针的高灵敏度，化合物的浓度低浓度。自从它 安装，光谱仪一直是每日服务的研究生和教职员工 校园各个领域的成员，以及邻近学术的定期用户 和政府研究机构。它一直是几种必不可少的工具 NIH资助计划的首席研究人员（PIS）。但是，光谱仪具有 近年来，由于经常维修其衰老，近年来开始增加停机时间 冷冻文化和冷冻-TXI探测器，破坏了用户的NMR研究活动。 我们认为，迫切需要解决的可靠性问题 光谱仪确保系统的可持续性和性能。提案 NIH资助的PI和其他教职员工都强烈支持更换 在UMCP。 Sig Grant将提供一个机会，以营救老化工具 并提高仪器的性能和能力。如果被授予 迄今为止的工具将为UMCP NMR社区带来以下利益：（1）确保 几个NIH资助的研究计划的进步。 （2）鼓舞新的生物分子 NMR研究兴趣。 （3）提高生产率和效率。 （4）提供动手 为研究生和博士后（5）的学习和工作经验（5） 减少液氮消耗/成本。