Metabolic & Molecular Physiology

NIH发起了集中专业知识、仪器和设施的努力，以促进全面的 对基因工程小鼠、组织和细胞的评估提高了我们对 功能基因组学和疾病病理学。代谢和分子生理学核心（MMPC） 为加州大学圣地亚哥分校，加州大学洛杉矶分校及其附属机构的调查人员提供了一系列最先进的 和成本有效的分子和生理测定法， 中心. MMPC分为四个子核心：A）胰岛素敏感性和代谢稳态，B） 动物和组织中的氧化代谢，C）细胞中的线粒体生物学和代谢，以及D） 炎症信号和糖尿病并发症，每个子核心提供广泛的培训和 就从实验设计到数据解释和整合的各种主题进行咨询。 具体而言，MMPC提供评估服务：运动，进食行为，间接热量测定， 身体组成、葡萄糖/胰岛素耐受性、胰岛素作用（离体、原位和体内）、底物 代谢和氧化能力，线粒体功能和形态，循环激素/ 脂肪因子/细胞因子和糖尿病并发症。MMPC维持组织生物库以及 一个关于各种基因工程小鼠表型结果的综合数据库 以及大量表型分析技术的标准方案。MMPC领导层包括 来自综合代谢、核受体生物学、线粒体 功能和结构，以及炎症，包括：Andrea Hevener，Orian Shirihai，Peter Tontonoz， 还有凯伦·罗伊MMPC的优势包括其全面和互补的专业知识， 领导力、卓越的研究生产力记录和高影响力的科学出版物。 MMPC团队的协作精神培养了一个合议的环境，并支持良好的服务- 与其他刚果民主共和国核心和机构资源协调。MMPC的中心目标是 提高DRC成员在前沿代谢分析方面的科学能力， 提高整体研究质量，加强从细胞，组织和 老鼠变成人。

NIH-initiated efforts to centralize expertise, instrumentation, and facilities to promote comprehensive evaluation of genetically engineered mice, tissues and cells has improved our understanding of functional genomics and disease pathobiology. The Metabolic and Molecular Physiology Core (MMPC) provides investigators at UCSD, UCLA, and their affiliated institutions with a series of state-of-the-art and cost effective molecular and physiological assays not readily available from national phenotyping centers. The MMPC is divided into four sub-cores: A) Insulin sensitivity and metabolic homeostasis, B) Oxidative metabolism in animals and tissues, C) Mitochondrial biology and metabolism in cells, and D) Inflammatory signaling and diabetes complications, and each sub-core offers extensive training and consultation on a variety of topics from experimental design to data interpretation and integration. Specifically, the MMPC provides services to assess: movement, feeding behavior, indirect calorimetry, body composition, glucose/insulin tolerance, insulin action (ex vivo, in situ, and in vivo), substrate metabolism and oxidative capacity, mitochondrial function and morphology, circulating hormones / adipokines / cytokines, and diabetes complications. The MMPC maintains a tissue bio-bank as well as a comprehensive database of phenotypic outcomes for a wide variety of genetically engineered mice and standard protocols for a vast number of phenotyping techniques. The MMPC leadership includes top investigators from the fields of integrative metabolism, nuclear receptor biology, mitochondrial function and architecture, and inflammation including: Andrea Hevener, Orian Shirihai, Peter Tontonoz, and Karen Reue. Strengths of the MMPC include the well-rounded and complementary expertise of its leadership, an exceptional track record of research productivity, and high impact scientific publications. The collaborative spirit of the MMPC team fosters a collegial environment and supports service well- coordinated with other DRC cores and institutional resources. The central goal of the MMPC is to advance the scientific capabilities of the DRC membership in leading-edge metabolic analyses and improve overall research quality with enhanced translation of research ideas from cells, tissues, and mice to (wo)man.