Genotoxic and mutagenic effects of combinations of e-cigarettes and tobacco carcinogens in mouse oral tissues

电子烟和烟草致癌物组合对小鼠口腔组织的基因毒性和致突变作用

• 批准号: 10179358
• 负责人: JOSEPH B GUTTENPLAN
• 金额: $ 20.23万
• 依托单位: NEW YORK UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-06-03 至 2023-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10179358
• 关键词: Adult; Affect; Age; Aldehydes; Aryl Hydrocarbon Receptor; Benefits and Risks; Benzo(a)pyrene; Biological Assay; Cancer Cell Growth; Carcinogen Metabolism; Carcinogens; Cell Cycle; Cell Proliferation; Cells; Cigarette; Consumption; Country; DNA Adduction; DNA Damage; Data; Electronic cigarette; Epidemiology; Exposure to; Formaldehyde; Future; Gene Expression; Genes; Health; Human; In Vitro; Individual; JUUL; Liquid substance; Malignant Neoplasms; Measures; Metabolism; Methods; Mus; Mutagenesis; N' -nitrosonornicotine; Nicotine; Nose; Oral; Oral cavity; Organ; Outcome; Paper; Pathway interactions; Pharmaceutical Preparations; Pilot Projects; Positioning Attribute; Prevalence; Process; Publishing; Reporter; Reporting; Research; Risk; Risk Factors; Route; Site; Smoker; Time; Tissues; Tobacco; Tobacco smoke; Tobacco smoking behavior; Tobacco-Associated Carcinogen; Tumor Promotion; Tumor-Associated Process; base; cancer cell; cancer risk; carcinogenesis; carcinogenicity; cigarette user; design; drug metabolism; e-cigarette aerosols; electronic cigarette use; electronic cigarette user; experience; experimental study; former smoker; genotoxicity; in vivo; malignant mouth neoplasm; mouse model; oral tissue; prototype; tobacco products; tobacco smokers; tobacco user; tumor initiators; tumor progression; tumorigenesis; vaper; vaping; vapor; vector; voltage

This is project is designed to determine whether the use of e-cigarettes (e-cigs) increases the risk for cancer of the oral cavity, especially when combined with tobacco smoke carcinogens. The proposed research will serve as a murine model for users of cigarettes and e-cigs (dual users). As e-cigs have only become popular wi t h i n the last decade and carcinogenesis occurs over decades, there has not been sufficient time for any epidemiological evidence one way or another. Yet e-cigs are currently being used by millions of individuals, and many of the same individuals are also tobacco smokers. In addition, others who are ex-smokers may already harbor precancer or cancer cells in the mouth, and other organs. The proposed research will investigate the effects of e-cig aerosol, tobacco carcinogens and their combinations on several outcomes relevant to carcinogenesis. . A nicotine-only control will be included. The outcomes consist of DNA damage, mutagenesis and changes in expression of critical genes and pathways in drug metabolism and the cell cycle. The use of the combination is to investigate possible cocarcinogenic or promoting effects of E-cigs. The oral cavity is a major site of exposure to e-cigs and there have been few if any reports on the potential carcinogenic effects of e-cig exposure on these sites. The results of this study are likely important and timely, as the experiments on the potential carcinogenic effects of the e-cigs cannot be studied in humans, yet the public is increasingly using e- cigs.

该项目旨在确定使用电子烟（e-cigs）是否会增加患癌症的风险 口腔，尤其是与烟草烟雾致癌物质混合时。拟议的研究将服务于 作为香烟和电子烟使用者（双重使用者）的小鼠模型。由于电子烟仅在 在过去的十年中，癌症的发生已经过去了几十年，但还没有足够的时间来进行任何研究 流行病学证据以一种或另一种方式。然而，目前电子烟已被数百万人使用，并且 其中许多人也是吸烟者。此外，其他戒烟者可能已经 口腔和其他器官中含有癌前细胞或癌细胞。拟议的研究将调查 电子烟气溶胶、烟草致癌物及其组合对与以下相关的几个结果的影响 致癌作用。 。将包括仅含尼古丁的对照品。结果包括 DNA 损伤、诱变 以及药物代谢和细胞周期中关键基因和途径表达的变化。使用 组合的目的是研究电子烟可能的致癌或促进作用。口腔是一个重要的部位 接触电子烟的场所，并且关于电子烟潜在致癌作用的报道很少（如果有的话） 在这些网站上的曝光。这项研究的结果可能是重要且及时的，因为在 电子烟的潜在致癌作用无法在人体中进行研究，但公众越来越多地使用电子烟 香烟。