Genotoxic and mutagenic effects of combinations of e-cigarettes and tobacco carcinogens in mouse oral tissues

电子烟和烟草致癌物组合对小鼠口腔组织的基因毒性和致突变作用

• 批准号: 10179358
• 负责人: JOSEPH B GUTTENPLAN
• 金额: $ 20.23万
• 依托单位: NEW YORK UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-06-03 至 2023-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10179358
• 关键词: Adult; Affect; Age; Aldehydes; Aryl Hydrocarbon Receptor; Benefits and Risks; Benzo(a)pyrene; Biological Assay; Cancer Cell Growth; Carcinogen Metabolism; Carcinogens; Cell Cycle; Cell Proliferation; Cells; Cigarette; Consumption; Country; DNA Adduction; DNA Damage; Data; Electronic cigarette; Epidemiology; Exposure to; Formaldehyde; Future; Gene Expression; Genes; Health; Human; In Vitro; Individual; JUUL; Liquid substance; Malignant Neoplasms; Measures; Metabolism; Methods; Mus; Mutagenesis; N' -nitrosonornicotine; Nicotine; Nose; Oral; Oral cavity; Organ; Outcome; Paper; Pathway interactions; Pharmaceutical Preparations; Pilot Projects; Positioning Attribute; Prevalence; Process; Publishing; Reporter; Reporting; Research; Risk; Risk Factors; Route; Site; Smoker; Time; Tissues; Tobacco; Tobacco smoke; Tobacco smoking behavior; Tobacco-Associated Carcinogen; Tumor Promotion; Tumor-Associated Process; base; cancer cell; cancer risk; carcinogenesis; carcinogenicity; cigarette user; design; drug metabolism; e-cigarette aerosols; electronic cigarette use; electronic cigarette user; experience; experimental study; former smoker; genotoxicity; in vivo; malignant mouth neoplasm; mouse model; oral tissue; prototype; tobacco products; tobacco smokers; tobacco user; tumor initiators; tumor progression; tumorigenesis; vaper; vaping; vapor; vector; voltage

This is project is designed to determine whether the use of e-cigarettes (e-cigs) increases the risk for cancer of the oral cavity, especially when combined with tobacco smoke carcinogens. The proposed research will serve as a murine model for users of cigarettes and e-cigs (dual users). As e-cigs have only become popular wi t h i n the last decade and carcinogenesis occurs over decades, there has not been sufficient time for any epidemiological evidence one way or another. Yet e-cigs are currently being used by millions of individuals, and many of the same individuals are also tobacco smokers. In addition, others who are ex-smokers may already harbor precancer or cancer cells in the mouth, and other organs. The proposed research will investigate the effects of e-cig aerosol, tobacco carcinogens and their combinations on several outcomes relevant to carcinogenesis. . A nicotine-only control will be included. The outcomes consist of DNA damage, mutagenesis and changes in expression of critical genes and pathways in drug metabolism and the cell cycle. The use of the combination is to investigate possible cocarcinogenic or promoting effects of E-cigs. The oral cavity is a major site of exposure to e-cigs and there have been few if any reports on the potential carcinogenic effects of e-cig exposure on these sites. The results of this study are likely important and timely, as the experiments on the potential carcinogenic effects of the e-cigs cannot be studied in humans, yet the public is increasingly using e- cigs.

该项目旨在确定使用电子烟（电子烟）是否增加了患癌症的风险 口腔，尤其是与烟草烟雾致癌物结合使用时。拟议的研究将服务 作为香烟和电子烟的用户（双用户）的鼠模型。由于电子烟只是在流行 过去的十年和致癌作用发生了数十年，没有足够的时间 流行病学证据一种或另一种方式。然而，目前数以百万计的人正在使用电子烟，并且 许多人也是吸烟者。此外，其他人可能已经 在口腔和其他器官中携带癌细胞或癌细胞。拟议的研究将调查 E-cig气溶胶，烟草致癌物及其组合对与几种相关结果的影响 致癌作用。 。将包括仅尼古丁的控制。结果包括DNA损伤，诱变 以及药物代谢和细胞周期中关键基因表达和途径的表达变化。使用 组合是研究可能的cocarcineg或促进电子烟的作用。口腔是主要的 暴露于电子烟的部位，几乎没有关于电子烟的潜在致癌作用的报道 在这些站点上接触。这项研究的结果可能很重要且及时，因为对 在人类中不能研究电子烟的潜在致癌作用，但是公众越来越多地使用e- 香烟。