Genotoxic and mutagenic effects of combinations of e-cigarettes and tobacco carcinogens in mouse oral tissues

该项目旨在确定电子烟(e-cigs)的使用是否会增加患癌症的风险 口腔，尤其是与烟草烟雾致癌物质结合时。这项拟议的研究将有助于 作为香烟和电子烟使用者(双重使用者)的小鼠模型。因为电子烟只在互联网上流行 在过去的十年里，癌症发生了几十年，没有足够的时间来进行任何 以某种方式提供流行病学证据。然而，电子烟目前正被数百万人使用，而且 这些人中的许多人也是烟民。此外，其他曾经吸烟的人可能已经 在口腔和其他器官中藏匿癌前病变或癌细胞。拟议的研究将调查 电子烟气雾剂、烟草致癌物及其组合对与以下几个结果相关的影响 致癌。。仅限尼古丁的对照将包括在内。结果包括DNA损伤，突变 以及药物代谢和细胞周期中关键基因和途径的表达变化。的用法 联合使用是为了研究E-CIG可能的致癌或促进作用。口腔是一种主要的 暴露于电子烟的场所，有关电子烟的潜在致癌作用的报道很少。 这些网站上的曝光率。这项研究的结果可能是重要的和及时的，因为在 电子烟的潜在致癌作用无法在人体内研究，但公众越来越多地使用电子烟。 雪茄。

This is project is designed to determine whether the use of e-cigarettes (e-cigs) increases the risk for cancer of the oral cavity, especially when combined with tobacco smoke carcinogens. The proposed research will serve as a murine model for users of cigarettes and e-cigs (dual users). As e-cigs have only become popular wi t h i n the last decade and carcinogenesis occurs over decades, there has not been sufficient time for any epidemiological evidence one way or another. Yet e-cigs are currently being used by millions of individuals, and many of the same individuals are also tobacco smokers. In addition, others who are ex-smokers may already harbor precancer or cancer cells in the mouth, and other organs. The proposed research will investigate the effects of e-cig aerosol, tobacco carcinogens and their combinations on several outcomes relevant to carcinogenesis. . A nicotine-only control will be included. The outcomes consist of DNA damage, mutagenesis and changes in expression of critical genes and pathways in drug metabolism and the cell cycle. The use of the combination is to investigate possible cocarcinogenic or promoting effects of E-cigs. The oral cavity is a major site of exposure to e-cigs and there have been few if any reports on the potential carcinogenic effects of e-cig exposure on these sites. The results of this study are likely important and timely, as the experiments on the potential carcinogenic effects of the e-cigs cannot be studied in humans, yet the public is increasingly using e- cigs.