The critical role of bromodomain proteins TgBDP1 and TgBDP2 in regulating gene expression in the protozoan parasite Toxoplasma gondii

对病原体的先天免疫应答涉及炎性细胞因子的表达和分泌。 有助于根除感染病原体的细胞因子。细胞因子失调 慢性炎症（chronic inflammation）可能对宿主产生负面影响。因此，一个基本的 了解调节炎症的分子机制对于发展 炎性疾病的治疗方法。我们确定了一个新的作用，转录因子 GLI 3在响应TLR-TRIF信号传导中调节细胞因子表达。单核细胞中的GLI 3 KO诱导 表明GLI 3与响应TLR-TRIF的炎症途径相关的表型 刺激.在这个建议中，我们将进一步阐明GLI 3在调节细胞因子表达中的作用， 单核细胞，并探讨这种调节在炎症反应中的相关性。

The innate immune response to pathogens involves the expression and secretion of inflammatory cytokines that act to facilitate eradication of the infecting pathogen. The dysregulation of cytokine production (chronic inflammation) can have negative effects on the host. Therefore, a fundamental understanding of the molecular mechanisms regulating inflammation is essential for the development of therapeutic approaches for inflammatory disorders. We identify a novel role for the transcription factor GLI3 in regulating cytokine expression in response to TLR-TRIF signaling. GLI3 KO in monocytes induces a phenotype that indicates GLI3 associates with inflammatory pathways in response to TLR-TRIF stimulation. In this proposal, we will further elucidate the role of GLI3 in regulating cytokine expression in monocytes and explore the relevance of this regulation in inflammatory responses.