The perception of odor blends and mixtures: Modulation, Inhibition and Enhancement of Olfactory Receptors alters perception of complex blends

对气味混合物和混合物的感知：嗅觉受体的调节、抑制和增强改变了对复杂混合物的感知

• 批准号: 10201117
• 负责人: Stuart J Firestein
• 金额: $ 20.25万
• 依托单位: COLUMBIA UNIV NEW YORK MORNINGSIDE
• 依托单位国家: 美国
• 财政年份: 2014
• 资助国家: 美国
• 起止时间: 2014-07-15 至 2024-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10201117
• 关键词: 2019-nCoV; Academic Medical Centers; Anosmia; Appearance; Cells; Cessation of life; Cilia; Complex; Disease; Dysgeusia; Fever; Fixatives; Freezing; Hour; Human; Immune response; Infection; Microscopy; Neurologic Effect; Neurologic Symptoms; Nose; Pathology; Pathway interactions; Patients; Perception; Procedures; Recovery; Reporting; Respiratory Signs and Symptoms; Smell Perception; Stains; Surgeon; Symptoms; Techniques; Time; Tissue Sample; Tissues; Viral; Virus; Virus Diseases; comorbidity; experience; human tissue; hyposmia; interest; olfactory receptor; pandemic disease; respiratory; tissue degeneration; tissue preparation; vascular abnormality

PROJECT SUMMARY/ABSTRACT This application seeks to take advantage of a unique opportunity to obtain significant amounts of human nasal tissue form recent CV-19 positive post mortems. Working with the Columbia University Medical Center Pathology Department and an ENY surgeon tissue is being recovered from post mortem procedures performed within 24 hours of death. We currently have 20 tissue samples of OE (attached to cribiform plate) and OB, in fixative and refrigerated or flash frozen. At the outset of the pandemic spread of the virus there were initially anecdotal, but now well quantified, reports of sudden anosmia and dysgeusia (likely a result of the anosmia) appearing 2-4 days before onset of the documented respiratory and fever symptoms of CV19. Although not all cases of CV19 experience the anosmia, all anosmias eventually became positive for CV19. The sense of smell is reportedly recovered in all surviving patients but requires 4-6 weeks post infection, long after other viral symptoms have resolved. The appearance of anosmia in the absence of respiratory symptoms – both before and after the disease time course, is of special interest as it differs significantly from hyposmias experienced during other respiratory illnesses. We will utilize human post mortem tissue to investigate, with advanced microscopy techniques and tissue preparation, several possible causes for this mysterious anosmia – including cell loss, tissue degeneration, cilia loss, vascular abnormalities, immune response comorbidities, and direct viral infection.

项目总结/摘要 本申请旨在利用一个独特的机会，以获得大量 从最近的CV-19阳性尸检中发现的人类鼻组织与哥伦比亚号合作 大学医学中心病理部门和ENY外科医生组织正在恢复 死亡后24小时内进行的尸检我们目前有20个 OE和OB的组织样本（附着在筛状板上），在固定剂中冷藏或快速冷冻 冻结在病毒大流行开始传播时，最初有轶事，但 现在已经很好地量化了，突然嗅觉丧失和味觉障碍的报告（可能是嗅觉丧失的结果） 在记录的CV19呼吸道和发热症状发作前2-4天出现。 虽然不是所有的CV19病例都会出现嗅觉丧失，但所有的嗅觉丧失最终都会成为 CV19阳性据报道，所有幸存患者的嗅觉都得到了恢复， 感染后需要4-6周，在其他病毒症状消失很久之后。的 在没有呼吸道症状的情况下出现嗅觉丧失-在 疾病的时间过程，是特别感兴趣的，因为它显着不同的hypospersistence经历 其他呼吸道疾病。我们将利用人体死后组织进行研究， 随着先进的显微镜技术和组织制备，这有几个可能的原因， 神秘的嗅觉丧失-包括细胞损失，组织变性，纤毛损失，血管异常， 免疫应答共病和直接病毒感染。