The perception of odor blends and mixtures: Modulation, Inhibition and Enhancement of Olfactory Receptors alters perception of complex blends

对气味混合物和混合物的感知：嗅觉受体的调节、抑制和增强改变了对复杂混合物的感知

• 批准号: 10201117
• 负责人: Stuart J Firestein
• 金额: $ 20.25万
• 依托单位: COLUMBIA UNIV NEW YORK MORNINGSIDE
• 依托单位国家: 美国
• 财政年份: 2014
• 资助国家: 美国
• 起止时间: 2014-07-15 至 2024-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10201117
• 关键词: 2019-nCoV; Academic Medical Centers; Anosmia; Appearance; Cells; Cessation of life; Cilia; Complex; Disease; Dysgeusia; Fever; Fixatives; Freezing; Hour; Human; Immune response; Infection; Microscopy; Neurologic Effect; Neurologic Symptoms; Nose; Pathology; Pathway interactions; Patients; Perception; Procedures; Recovery; Reporting; Respiratory Signs and Symptoms; Smell Perception; Stains; Surgeon; Symptoms; Techniques; Time; Tissue Sample; Tissues; Viral; Virus; Virus Diseases; comorbidity; experience; human tissue; hyposmia; interest; olfactory receptor; pandemic disease; respiratory; tissue degeneration; tissue preparation; vascular abnormality

PROJECT SUMMARY/ABSTRACT This application seeks to take advantage of a unique opportunity to obtain significant amounts of human nasal tissue form recent CV-19 positive post mortems. Working with the Columbia University Medical Center Pathology Department and an ENY surgeon tissue is being recovered from post mortem procedures performed within 24 hours of death. We currently have 20 tissue samples of OE (attached to cribiform plate) and OB, in fixative and refrigerated or flash frozen. At the outset of the pandemic spread of the virus there were initially anecdotal, but now well quantified, reports of sudden anosmia and dysgeusia (likely a result of the anosmia) appearing 2-4 days before onset of the documented respiratory and fever symptoms of CV19. Although not all cases of CV19 experience the anosmia, all anosmias eventually became positive for CV19. The sense of smell is reportedly recovered in all surviving patients but requires 4-6 weeks post infection, long after other viral symptoms have resolved. The appearance of anosmia in the absence of respiratory symptoms – both before and after the disease time course, is of special interest as it differs significantly from hyposmias experienced during other respiratory illnesses. We will utilize human post mortem tissue to investigate, with advanced microscopy techniques and tissue preparation, several possible causes for this mysterious anosmia – including cell loss, tissue degeneration, cilia loss, vascular abnormalities, immune response comorbidities, and direct viral infection.

项目摘要/摘要 这项申请寻求利用一个独特的机会来获得可观的金额 人的鼻部组织形成了最近的CV-19阳性尸检。与哥伦比亚大学合作 大学医学中心病理科和一名ENY外科医生的组织正在被发现 在死亡后24小时内进行的验尸程序。我们目前有20个 OE(附在筛形板上)和OB的组织样品，放在固定剂中，冷藏或闪蒸 冻住了。在病毒大流行传播之初，最初有一些轶事，但 现在已经很好地量化了突发性嗅觉障碍和感觉障碍的报告(很可能是嗅觉障碍的结果) 出现在记录在案的CV19呼吸道和发烧症状出现前2-4天。 虽然并不是所有的CV19病例都经历了嗅觉缺失，但所有的嗅觉缺失最终都变成了 CV19检测呈阳性。据报道，所有幸存的患者都恢复了嗅觉，但 需要在感染后4-6周，在其他病毒症状消失很久之后。这个 在没有呼吸道症状的情况下出现嗅觉障碍-无论是在治疗前后 疾病的时间进程特别令人感兴趣，因为它与所经历的尿道下裂有显著的不同 在其他呼吸道疾病期间。我们将利用人类尸检组织进行调查， 有了先进的显微镜技术和组织准备，造成这种情况的几个可能原因 神秘的嗅觉丧失-包括细胞丢失，组织退化，纤毛丢失，血管异常， 免疫反应并存，以及直接病毒感染。