Molecular Resources and Services Module

分子资源和服务模块

• 批准号: 10197136
• 负责人: JOHN O'BRIEN
• 金额: $ 18.54万
• 依托单位: UNIVERSITY OF TEXAS HLTH SCI CTR HOUSTON
• 依托单位国家: 美国
• 财政年份: 2017
• 资助国家: 美国
• 起止时间: 2017-09-01 至 2024-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10197136
• 关键词: Animal Model; Antibodies; Area; Cell Culture Techniques; Cell Line; Cells; Clustered Regularly Interspaced Short Palindromic Repeats; Collaborations; Computer software; Consultations; Cultured Cells; Development; Equipment; Experimental Designs; Faculty; Genes; Genetically Modified Animals; Individual; Laboratories; Laboratory culture; Lentivirus; Molecular; Molecular Analysis; Molecular Biology; Pilot Projects; RNA Interference; Reagent; Research; Research Personnel; Research Project Grants; Resources; Services; Small Interfering RNA; Source; Specificity; Techniques; Tissues; Validation; Viral Packaging; Viral Vector; Virus; Vision research; Work; adeno-associated viral vector; base; design; design and construction; experience; experimental study; genetic manipulation; interest; investigator training; member; optogenetics; tissue culture; tool; vector; virus development

MOLECULAR RESOURCES AND SERVICES MODULE ABSTRACT The Molecular Resources and Services Module provides centralized resources and a variety of specialized services to members of the Vision Research Group to support projects employing cellular and molecular techniques. Core equipment and software supported by the Module is freely available to Vision Research Group investigators, providing most basic needs for molecular work, eliminating the need for redundant equipment and software purchases, and allowing new investigators and investigators initiating new projects to begin work quickly. The Module maintains a well-equipped cell culture laboratory with an experienced technician. Trained investigators may use the laboratory or investigators may use the services provided by the technician to perform experiments with cell lines, primary cells, and cultured tissues. Module faculty provide two high-level services to Vision Research Group investigators. Through a virus development and packaging service we will design and construct lentivirus or adeno-associated virus vectors to deliver gene constructs of interest to animal models. We will also provide a service to validate infectivity and cargo of viral vectors obtained from outside sources. Through a molecular reagent validation service, we will design and perform experiments to validate the specificity of reagents such as antibodies and CRISPR constructs.

分子资源和服务部 摘要 分子资源和服务模块提供集中的资源和各种专门的 为视觉研究小组的成员提供服务，以支持使用细胞和分子的项目。 技术.该模块支持的核心设备和软件可免费提供给Vision Research 小组调查员，提供分子工作的最基本需求，消除了冗余 设备和软件采购，并允许新的调查人员和调查人员启动新的项目， 快点开始工作。该单元拥有一个设备齐全的细胞培养实验室， 技师经过培训的研究者可以使用实验室，或者研究者可以使用 技术人员使用细胞系、原代细胞和培养的组织进行实验。模块教师提供 两个高层次的服务，以视觉研究小组的调查人员。通过病毒的开发和包装 服务，我们将设计和构建慢病毒或腺相关病毒载体，以提供基因结构， 动物模型的兴趣。我们还将提供服务，以验证病毒载体的感染性和货物 从外部来源获得。通过分子试剂验证服务，我们将设计并执行 验证抗体和CRISPR构建体等试剂特异性的实验。