Targeting DEPTOR in multiple myeloma

项目概要/摘要 初步工作表明 mTOR 结合蛋白 DEPTOR 是治疗以下疾病的潜在分子靶点 多发性骨髓瘤。它在这种疾病中特别上调，并且它的沉默对于骨髓瘤细胞来说是致命的。我们， 因此，在高通量药物筛选中鉴定出一种潜在的 DEPTOR 抑制剂。该抑制剂有效地 骨髓瘤细胞具有预期的分子改变和诱导死亡。在本提案中，我们计划 进一步开发这种抑制剂。我们将生产生化衍生物，旨在增强 DEPTOR 抑制剂的治疗指数，确定抑制剂首先结合的作用机制 骨髓瘤细胞，确定 DEPTOR 表达缺失的小鼠对正常组织的可能毒性，以及 评估与临床使用的其他抗骨髓瘤治疗药物可能存在的相互作用。我们的期望是 这些新药将成为骨髓瘤患者治疗药物的重要补充， 此外，它们还将专门针对具有高 DEPTOR 表达的肿瘤患者。

PROJECT SUMMARY/ABSTRACT Preliminary work indicates the mTOR-binding protein DEPTOR is a potential molecular target for therapy in multiple myeloma. It is specifically upregulated in this disease and its silencing is lethal to myeloma cells. We, thus, identified a potential DEPTOR inhibitor in a high-throughput drug screen. The inhibitor is effective in myeloma cells with the expected molecular alterations and induction of death. In this proposal we plan to further develop this inhibitor. We will generate biochemical derivatives with the aim of enhancing the therapeutic index of DEPTOR inhibitors, identify the mechanism of action of where the inhibitors first bind in myeloma cells, ascertain possible toxicity to normal tissues in mice with deleted DEPTOR expression and assess possible interactions with other anti-myeloma therapeutics that are used in the clinic. Our expectation is that these new drugs will be important additions to the armamentarium used in myeloma patients and, furthermore, they will be specifically tailored to patients with tumors that have high DEPTOR expression.